Development of an antioxidant assay to study oxidative
potential of airborne particulate matter

J 2019

Development of an antioxidant assay to study oxidative potential of airborne particulate matter

SHAHPOURY, P., T. HARNER, Gerhard LAMMEL, S. LELIEVELD, H.J. TONG et. al.

Basic information

Original name

Development of an antioxidant assay to study oxidative potential of airborne particulate matter

Authors

SHAHPOURY, P., T. HARNER, Gerhard LAMMEL, S. LELIEVELD, H.J. TONG and J. WILSON

Edition

ATMOSPHERIC MEASUREMENT TECHNIQUES, GOTTINGEN, COPERNICUS GESELLSCHAFT MBH, 2019, 1867-1381

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10509 Meteorology and atmospheric sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.668

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.5194/amt-12-6529-2019

UT WoS

000502261600006

Keywords in English

PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMIC-LIKE SUBSTANCES; WATER-SOLUBLE PM2.5; REDOX STATE; DITHIOTHREITOL DTT; AIR-POLLUTION; STRESS; GLUTATHIONE; HEALTH; SURFACTANT

Změněno: 15/4/2020 18:01, Mgr. Marie Šípková, DiS.

Abstract

V originále

Oxidative potential is a measure of redox activity of airborne particulate matter (PM) and is often used as a surrogate to estimate one form of PM toxicity. The evaluation of oxidative potential in a physiologically relevant environment is always challenging. In this work, we developed a chromatographic method, employing an ultra-high-performance liquid chromatograph coupled to a triple-quadruple mass spectrometer, to determine the oxidative potential of PM from different sources. To this purpose, we measured the PM-induced oxidation of glutathione, cysteine, and ascorbic acid, and formation of glutathione disulfide and cystine, following PM addition to simulated epithelial lining fluids, which, in addition to the antioxidants, contained inorganic salts, a phospholipid, and proteins. The new method showed high precision and, when applied to standard reference PM, the oxidative potential was found to increase with the reaction time and PM concentration in the lung fluid. The antioxidant depletion rates were considerably higher than the rates found with the conventional dithiothreitol assay, indicating the higher sensitivity of the new method. The presence of the lung fluid inorganic species increased the oxidative potential determined through glutathione and cysteine, but showed an opposite effect with ascorbic acid, whereas the presence of proteins resulted in a moderate decrease in the oxidative potential. In the presence of PM2.5, glutathione and cysteine demonstrated similar depletion patterns, which were noticeably different from that of ascorbic acid, suggesting that cysteine could be used as an alternative to glutathione for probing oxidative potential.

Citovat

SHAHPOURY, P., T. HARNER, Gerhard LAMMEL, S. LELIEVELD, H.J. TONG and J. WILSON. Development of an antioxidant assay to study oxidative potential of airborne particulate matter. ATMOSPHERIC MEASUREMENT TECHNIQUES. GOTTINGEN: COPERNICUS GESELLSCHAFT MBH, 2019, vol. 12, No 12, p. 6529-6539. ISSN 1867-1381. Available from: https://dx.doi.org/10.5194/amt-12-6529-2019.

@article{1645957,
   author = {Shahpoury, P. and Harner, T. and Lammel, Gerhard and Lelieveld, S. and Tong, H.J. and Wilson, J.},
   article_location = {GOTTINGEN},
   article_number = {12},
   doi = {http://dx.doi.org/10.5194/amt-12-6529-2019},
   keywords = {PERFORMANCE LIQUID-CHROMATOGRAPHY; HUMIC-LIKE SUBSTANCES; WATER-SOLUBLE PM2.5; REDOX STATE; DITHIOTHREITOL DTT; AIR-POLLUTION; STRESS; GLUTATHIONE; HEALTH; SURFACTANT},
   language = {eng},
   issn = {1867-1381},
   journal = {ATMOSPHERIC MEASUREMENT TECHNIQUES},
   note = {Žádný z autorů nemá afiliaci na MU.},
   title = {Development of an antioxidant assay to study oxidative potential of airborne particulate matter},
   url = {https://www.atmos-meas-tech.net/12/6529/2019/},
   volume = {12},
   year = {2019}
}

TY  - JOUR
ID  - 1645957
AU  - Shahpoury, P. - Harner, T. - Lammel, Gerhard - Lelieveld, S. - Tong, H.J. - Wilson, J.
PY  - 2019
TI  - Development of an antioxidant assay to study oxidative potential of airborne particulate matter
JF  - ATMOSPHERIC MEASUREMENT TECHNIQUES
VL  - 12
IS  - 12
SP  - 6529-6539
EP  - 6529-6539
PB  - COPERNICUS GESELLSCHAFT MBH
SN  - 18671381
N1  - Žádný z autorů nemá afiliaci na MU.
KW  - PERFORMANCE LIQUID-CHROMATOGRAPHY
KW  - HUMIC-LIKE SUBSTANCES
KW  - WATER-SOLUBLE PM2.5
KW  - REDOX STATE
KW  - DITHIOTHREITOL DTT
KW  - AIR-POLLUTION
KW  - STRESS
KW  - GLUTATHIONE
KW  - HEALTH
KW  - SURFACTANT
UR  - https://www.atmos-meas-tech.net/12/6529/2019/
N2  - Oxidative potential is a measure of redox activity of airborne particulate matter (PM) and is often used as a surrogate to estimate one form of PM toxicity. The evaluation of oxidative potential in a physiologically relevant environment is always challenging. In this work, we developed a chromatographic method, employing an ultra-high-performance liquid chromatograph coupled to a triple-quadruple mass spectrometer, to determine the oxidative potential of PM from different sources. To this purpose, we measured the PM-induced oxidation of glutathione, cysteine, and ascorbic acid, and formation of glutathione disulfide and cystine, following PM addition to simulated epithelial lining fluids, which, in addition to the antioxidants, contained inorganic salts, a phospholipid, and proteins. The new method showed high precision and, when applied to standard reference PM, the oxidative potential was found to increase with the reaction time and PM concentration in the lung fluid. The antioxidant depletion rates were considerably higher than the rates found with the conventional dithiothreitol assay, indicating the higher sensitivity of the new method. The presence of the lung fluid inorganic species increased the oxidative potential determined through glutathione and cysteine, but showed an opposite effect with ascorbic acid, whereas the presence of proteins resulted in a moderate decrease in the oxidative potential. In the presence of PM2.5, glutathione and cysteine demonstrated similar depletion patterns, which were noticeably different from that of ascorbic acid, suggesting that cysteine could be used as an alternative to glutathione for probing oxidative potential.
ER  -

SHAHPOURY, P., T. HARNER, Gerhard LAMMEL, S. LELIEVELD, H.J. TONG and J. WILSON. Development of an antioxidant assay to study oxidative potential of airborne particulate matter. \textit{ATMOSPHERIC MEASUREMENT TECHNIQUES}. GOTTINGEN: COPERNICUS GESELLSCHAFT MBH, 2019, vol.~12, No~12, p.~6529-6539. ISSN~1867-1381. Available from: https://dx.doi.org/10.5194/amt-12-6529-2019.

Detailed Information on Publication Record