J 2020

Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry

FARKA, Zdeněk, Matthias Jürgen MICKERT, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK, Pavla BOUCHALOVÁ et. al.

Basic information

Original name

Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry

Authors

FARKA, Zdeněk (203 Czech Republic, guarantor, belonging to the institution), Matthias Jürgen MICKERT (276 Germany), Zuzana MIKUŠOVÁ (703 Slovakia, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Pavla BOUCHALOVÁ (203 Czech Republic, belonging to the institution), Wenshu XU, Pavel BOUCHAL (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Hans-Heiner GORRIS (276 Germany)

Edition

Nanoscale, Cambridge, Royal Society of Chemistry, 2020, 2040-3364

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21001 Nano-materials

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 7.790

RIV identification code

RIV/00216224:14740/20:00114104

Organization unit

Central European Institute of Technology

DOI

UT WoS

000529201500023

Keywords in English

photon-upconversion nanoparticle; immunocytochemistry; immunocytochemistry; bioconjugation; surface modification; streptavidin

Tags

Tags

International impact, Reviewed
Změněno: 9/10/2024 12:55, Ing. Martina Blahová

Abstract

V originále

Immunohistochemistry (IHC) and immunocytochemistry (ICC) are routinely employed for the microscopic identification and diagnosis of cancerous cells in histological tissues and cell cultures. The maximally attainable contrast of conventional histological staining techniques, however, is low. While the anti-Stokes emission of photon-upconversion nanoparticles (UCNP) can efficiently eliminate optical background interference, excluding non-specific interactions of the label with the histological sample is equally important for specific immunolabeling. To address both requirements, we have designed and characterized several UCNP-based nanoconjugates as labels for the highly specific detection of the cancer biomarker HER2 on various breast cancer cell lines. An optimized streptavidin-PEG-neridronate-UCNP conjugate provided an unsurpassed signal-to-background ratio of 319, which was 50-fold better than conventional fluorescent labeling under the same experimental conditions. In combination, the absence of optical interference and non-specific binding lays the foundation for computer-based data evaluation in digital pathology.

Links

GA17-05957S, research and development project
Name: Evaluace nových potenciálních cílů a inhibitorů pro blokování vývoje metastáz u luminálních A nádorů prsu
Investor: Czech Science Foundation
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LTAB19011, research and development project
Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel
Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION
MUNI/A/1252/2019, interní kód MU
Name: Podpora biochemického výzkumu v roce 2020
Investor: Masaryk University, Category A
90043, large research infrastructures
Name: CIISB