FARKA, Zdeněk, Matthias Jürgen MICKERT, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK, Pavla BOUCHALOVÁ, Wenshu XU, Pavel BOUCHAL, Petr SKLÁDAL and Hans-Heiner GORRIS. Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry. Nanoscale. Cambridge: Royal Society of Chemistry, 2020, vol. 12, No 15, p. 8303-8313. ISSN 2040-3364. Available from: https://dx.doi.org/10.1039/C9NR10568A.
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Basic information
Original name Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry
Authors FARKA, Zdeněk (203 Czech Republic, guarantor, belonging to the institution), Matthias Jürgen MICKERT (276 Germany), Zuzana MIKUŠOVÁ (703 Slovakia, belonging to the institution), Antonín HLAVÁČEK (203 Czech Republic), Pavla BOUCHALOVÁ (203 Czech Republic, belonging to the institution), Wenshu XU, Pavel BOUCHAL (203 Czech Republic, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Hans-Heiner GORRIS (276 Germany).
Edition Nanoscale, Cambridge, Royal Society of Chemistry, 2020, 2040-3364.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 21001 Nano-materials
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 7.790
RIV identification code RIV/00216224:14740/20:00114104
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1039/C9NR10568A
UT WoS 000529201500023
Keywords in English photon-upconversion nanoparticle; immunocytochemistry; immunocytochemistry; bioconjugation; surface modification; streptavidin
Tags CF CRYO, CF PROT, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 9/3/2021 14:31.
Abstract
Immunohistochemistry (IHC) and immunocytochemistry (ICC) are routinely employed for the microscopic identification and diagnosis of cancerous cells in histological tissues and cell cultures. The maximally attainable contrast of conventional histological staining techniques, however, is low. While the anti-Stokes emission of photon-upconversion nanoparticles (UCNP) can efficiently eliminate optical background interference, excluding non-specific interactions of the label with the histological sample is equally important for specific immunolabeling. To address both requirements, we have designed and characterized several UCNP-based nanoconjugates as labels for the highly specific detection of the cancer biomarker HER2 on various breast cancer cell lines. An optimized streptavidin-PEG-neridronate-UCNP conjugate provided an unsurpassed signal-to-background ratio of 319, which was 50-fold better than conventional fluorescent labeling under the same experimental conditions. In combination, the absence of optical interference and non-specific binding lays the foundation for computer-based data evaluation in digital pathology.
Links
GA17-05957S, research and development projectName: Evaluace nových potenciálních cílů a inhibitorů pro blokování vývoje metastáz u luminálních A nádorů prsu
Investor: Czech Science Foundation
LM2015043, research and development projectName: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development projectName: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LTAB19011, research and development projectName: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel
Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION
MUNI/A/1252/2019, interní kód MUName: Podpora biochemického výzkumu v roce 2020
Investor: Masaryk University, Category A
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