| FARKA, Zdeněk, Matthias Jürgen MICKERT, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK, Pavla BOUCHALOVÁ, Wenshu XU, Pavel BOUCHAL, Petr SKLÁDAL and Hans-Heiner GORRIS. Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry. Nanoscale. Cambridge: Royal Society of Chemistry, 2020, vol. 12, No 15, p. 8303-8313. ISSN 2040-3364. Available from: https://dx.doi.org/10.1039/C9NR10568A. |
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@article{1646558,
author = {Farka, Zdeněk and Mickert, Matthias Jürgen and Mikušová, Zuzana and Hlaváček, Antonín and Bouchalová, Pavla and Xu, Wenshu and Bouchal, Pavel and Skládal, Petr and Gorris, HansandHeiner},
article_location = {Cambridge},
article_number = {15},
doi = {http://dx.doi.org/10.1039/C9NR10568A},
keywords = {photon-upconversion nanoparticle; immunocytochemistry; immunocytochemistry; bioconjugation; surface modification; streptavidin},
language = {eng},
issn = {2040-3364},
journal = {Nanoscale},
title = {Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry},
url = {https://pubs.rsc.org/en/content/articlelanding/2020/nr/c9nr10568a},
volume = {12},
year = {2020}
}
TY - JOUR
ID - 1646558
AU - Farka, Zdeněk - Mickert, Matthias Jürgen - Mikušová, Zuzana - Hlaváček, Antonín - Bouchalová, Pavla - Xu, Wenshu - Bouchal, Pavel - Skládal, Petr - Gorris, Hans-Heiner
PY - 2020
TI - Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry
JF - Nanoscale
VL - 12
IS - 15
SP - 8303-8313
EP - 8303-8313
PB - Royal Society of Chemistry
SN - 20403364
KW - photon-upconversion nanoparticle
KW - immunocytochemistry
KW - immunocytochemistry
KW - bioconjugation
KW - surface modification
KW - streptavidin
UR - https://pubs.rsc.org/en/content/articlelanding/2020/nr/c9nr10568a
L2 - https://pubs.rsc.org/en/content/articlelanding/2020/nr/c9nr10568a
N2 - Immunohistochemistry (IHC) and immunocytochemistry (ICC) are routinely employed for the microscopic identification and diagnosis of cancerous cells in histological tissues and cell cultures. The maximally attainable contrast of conventional histological staining techniques, however, is low. While the anti-Stokes emission of photon-upconversion nanoparticles (UCNP) can efficiently eliminate optical background interference, excluding non-specific interactions of the label with the histological sample is equally important for specific immunolabeling. To address both requirements, we have designed and characterized several UCNP-based nanoconjugates as labels for the highly specific detection of the cancer biomarker HER2 on various breast cancer cell lines. An optimized streptavidin-PEG-neridronate-UCNP conjugate provided an unsurpassed signal-to-background ratio of 319, which was 50-fold better than conventional fluorescent labeling under the same experimental conditions. In combination, the absence of optical interference and non-specific binding lays the foundation for computer-based data evaluation in digital pathology.
ER -
FARKA, Zdeněk, Matthias Jürgen MICKERT, Zuzana MIKUŠOVÁ, Antonín HLAVÁČEK, Pavla BOUCHALOVÁ, Wenshu XU, Pavel BOUCHAL, Petr SKLÁDAL and Hans-Heiner GORRIS. Surface design of photon-upconversion nanoparticles for high-contrast immunocytochemistry. \textit{Nanoscale}. Cambridge: Royal Society of Chemistry, 2020, vol.~12, No~15, p.~8303-8313. ISSN~2040-3364. Available from: https://dx.doi.org/10.1039/C9NR10568A.
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