Detailed Information on Publication Record
2017
Nonstructural carbohydrate-balance response to long-term elevated CO2 exposure in European beech and Norway spruce mixed cultures: biochemical and ultrastructural responses
MASKOVA, P., B. RADOCHOVA, Z. LHOTAKOVA, Jan MICHÁLEK, H. LIPAVSKA et. al.Basic information
Original name
Nonstructural carbohydrate-balance response to long-term elevated CO2 exposure in European beech and Norway spruce mixed cultures: biochemical and ultrastructural responses
Authors
MASKOVA, P., B. RADOCHOVA, Z. LHOTAKOVA, Jan MICHÁLEK (203 Czech Republic, guarantor, belonging to the institution) and H. LIPAVSKA
Edition
Canadian journal of forest research. Ottawa, The national research council, 2017, 0045-5067
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
40102 Forestry
Country of publisher
Canada
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.887
RIV identification code
RIV/00216224:14330/17:00113756
Organization unit
Faculty of Informatics
UT WoS
000413924100007
Keywords in English
chloroplast ultrastructure; CO2 enrichment; forest trees; soluble carbohydrates; starch
Tags
International impact, Reviewed
Změněno: 23/4/2020 16:00, Mgr. Marie Šípková, DiS.
Abstract
V originále
Two dominant central European tree species (Fagus sylvatica L. and Picea abies (L.) Karst.), in a mixed culture in semi-open glass domes, were used to simulate the reaction of forests to long-term elevated CO2 (EC) in a mountainous area (Beskydy Mountains, the Czech Republic). We investigated the effects of EC on soluble carbohydrate levels and composition. Starch content was evaluated using two methods: biochemical (glucose content after enzymatic hydrolysis) and stereological (starch grain proportion, size, and number in chloroplasts). In beech and spruce foliage, no significant changes in total soluble carbohydrate levels were observed. In spruce, starch content determined biochemically increased under EC, whereas no changes were detected in beech. The starch content determined stereologically increased only in beech. In spruce, EC exposure caused comparable starch increases in current-year and previous-year needles, although the former had a higher starch content and numerous larger starch grains regardless of CO2 concentration. In both species, the biochemical determination of carbohydrates exhibited greater individual tree uniformity, in contrast to large intraspecies variability. No changes in leaf soluble carbohydrates under long-term elevated CO2 demonstrate the ability of the studied tree species to efficiently allocate the photosynthates among the sinks. Thus, no photosynthetic downregulation via carbohydrate-level signalling can be expected.