J 2017

Nonstructural carbohydrate-balance response to long-term elevated CO2 exposure in European beech and Norway spruce mixed cultures: biochemical and ultrastructural responses

MASKOVA, P., B. RADOCHOVA, Z. LHOTAKOVA, Jan MICHÁLEK, H. LIPAVSKA et. al.

Basic information

Original name

Nonstructural carbohydrate-balance response to long-term elevated CO2 exposure in European beech and Norway spruce mixed cultures: biochemical and ultrastructural responses

Authors

MASKOVA, P., B. RADOCHOVA, Z. LHOTAKOVA, Jan MICHÁLEK (203 Czech Republic, guarantor, belonging to the institution) and H. LIPAVSKA

Edition

Canadian journal of forest research. Ottawa, The national research council, 2017, 0045-5067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40102 Forestry

Country of publisher

Canada

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.887

RIV identification code

RIV/00216224:14330/17:00113756

Organization unit

Faculty of Informatics

UT WoS

000413924100007

Keywords in English

chloroplast ultrastructure; CO2 enrichment; forest trees; soluble carbohydrates; starch

Tags

International impact, Reviewed
Změněno: 23/4/2020 16:00, Mgr. Marie Šípková, DiS.

Abstract

V originále

Two dominant central European tree species (Fagus sylvatica L. and Picea abies (L.) Karst.), in a mixed culture in semi-open glass domes, were used to simulate the reaction of forests to long-term elevated CO2 (EC) in a mountainous area (Beskydy Mountains, the Czech Republic). We investigated the effects of EC on soluble carbohydrate levels and composition. Starch content was evaluated using two methods: biochemical (glucose content after enzymatic hydrolysis) and stereological (starch grain proportion, size, and number in chloroplasts). In beech and spruce foliage, no significant changes in total soluble carbohydrate levels were observed. In spruce, starch content determined biochemically increased under EC, whereas no changes were detected in beech. The starch content determined stereologically increased only in beech. In spruce, EC exposure caused comparable starch increases in current-year and previous-year needles, although the former had a higher starch content and numerous larger starch grains regardless of CO2 concentration. In both species, the biochemical determination of carbohydrates exhibited greater individual tree uniformity, in contrast to large intraspecies variability. No changes in leaf soluble carbohydrates under long-term elevated CO2 demonstrate the ability of the studied tree species to efficiently allocate the photosynthates among the sinks. Thus, no photosynthetic downregulation via carbohydrate-level signalling can be expected.