KIF14 controls ciliogenesis via regulation of Aurora A and is
important for Hedgehog signaling

J 2020

KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling

PEJŠKOVÁ, Petra, Madeline Louise REILLY, Lucia BINÓ, Ondřej BERNATÍK, Linda DOLANSKÁ et. al.

Basic information

Original name

KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling

Authors

PEJŠKOVÁ, Petra (203 Czech Republic, belonging to the institution), Madeline Louise REILLY (250 France), Lucia BINÓ (703 Slovakia, belonging to the institution), Ondřej BERNATÍK (203 Czech Republic, belonging to the institution), Linda DOLANSKÁ (203 Czech Republic, belonging to the institution), Sri Ranjani GANJI (356 India), Zbyněk ZDRÁHAL (203 Czech Republic), Alexandre BENMERAH (250 France) and Lukáš ČAJÁNEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF CELL BIOLOGY, New York, ROCKEFELLER UNIVERSITY PRESS, 2020, 0021-9525

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 10.539

RIV identification code

RIV/00216224:14110/20:00114130

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1083/jcb.201904107

UT WoS

000538141100021

Keywords in English

PRIMARY CILIUM; MOTHER CENTRIOLE; MOTOR PROTEINS; SONIC HEDGEHOG; EARLY STEPS; KINESIN-II; CELL-CYCLE; A KINASE; ACTIVATION; CEP164

Abstract

V originále

Primary cilia play critical roles in development and disease. Their assembly and disassembly are tightly coupled to cell cycle progression. Here, we present data identifying KIF14 as a regulator of cilia formation and Hedgehog (HH) signaling. We show that RNAi depletion of KIF14 specifically leads to defects in ciliogenesis and basal body (BB) biogenesis, as its absence hampers the efficiency of primary cilium formation and the dynamics of primary cilium elongation, and disrupts the localization of the distal appendage proteins SCLT1 and FBF1 and components of the IFT-B complex. We identify deregulated Aurora A activity as a mechanism contributing to the primary cilium and BB formation defects seen after KIF14 depletion. In addition, we show that primary cilia in KIF14-depleted cells are defective in response to HH pathway activation, independently of the effects of Aurora A. In sum, our data point to KIF14 as a critical node connecting cell cycle machinery, effective ciliogenesis, and HH signaling.

Links

GA19-05244S, research and development project

Name: Molekulární aspekty ciliogeneze: zaměření na Tau Tubulin Kinázu 2

Investor: Czech Science Foundation

GJ16-03269Y, research and development project

Name: Tau tubulin kináza 2 v ciliogenezi: molekulární mechanismy a funkční důsledky

Investor: Czech Science Foundation

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

PEJŠKOVÁ, Petra, Madeline Louise REILLY, Lucia BINÓ, Ondřej BERNATÍK, Linda DOLANSKÁ, Sri Ranjani GANJI, Zbyněk ZDRÁHAL, Alexandre BENMERAH and Lukáš ČAJÁNEK. KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling. JOURNAL OF CELL BIOLOGY. New York: ROCKEFELLER UNIVERSITY PRESS, 2020, vol. 219, No 6, p. "1-23"-"S1-S6", 29 pp. ISSN 0021-9525. Available from: https://dx.doi.org/10.1083/jcb.201904107.

@article{1654416,
   author = {Pejšková, Petra and Reilly, Madeline Louise and Binó, Lucia and Bernatík, Ondřej and Dolanská, Linda and Ganji, Sri Ranjani and Zdráhal, Zbyněk and Benmerah, Alexandre and Čajánek, Lukáš},
   article_location = {New York},
   article_number = {6},
   doi = {http://dx.doi.org/10.1083/jcb.201904107},
   keywords = {PRIMARY CILIUM; MOTHER CENTRIOLE; MOTOR PROTEINS; SONIC HEDGEHOG; EARLY STEPS; KINESIN-II; CELL-CYCLE; A KINASE; ACTIVATION; CEP164},
   language = {eng},
   issn = {0021-9525},
   journal = {JOURNAL OF CELL BIOLOGY},
   title = {KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling},
   url = {https://rupress.org/jcb/article/219/6/e201904107/151721/KIF14-controls-ciliogenesis-via-regulation-of?searchresult=1},
   volume = {219},
   year = {2020}
}

TY  - JOUR
ID  - 1654416
AU  - Pejšková, Petra - Reilly, Madeline Louise - Binó, Lucia - Bernatík, Ondřej - Dolanská, Linda - Ganji, Sri Ranjani - Zdráhal, Zbyněk - Benmerah, Alexandre - Čajánek, Lukáš
PY  - 2020
TI  - KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling
JF  - JOURNAL OF CELL BIOLOGY
VL  - 219
IS  - 6
SP  - "1-23"-"S1-S6"
EP  - "1-23"-"S1-S6"
PB  - ROCKEFELLER UNIVERSITY PRESS
SN  - 00219525
KW  - PRIMARY CILIUM
KW  - MOTHER CENTRIOLE
KW  - MOTOR PROTEINS
KW  - SONIC HEDGEHOG
KW  - EARLY STEPS
KW  - KINESIN-II
KW  - CELL-CYCLE
KW  - A KINASE
KW  - ACTIVATION
KW  - CEP164
UR  - https://rupress.org/jcb/article/219/6/e201904107/151721/KIF14-controls-ciliogenesis-via-regulation-of?searchresult=1
L2  - https://rupress.org/jcb/article/219/6/e201904107/151721/KIF14-controls-ciliogenesis-via-regulation-of?searchresult=1
N2  - Primary cilia play critical roles in development and disease. Their assembly and disassembly are tightly coupled to cell cycle progression. Here, we present data identifying KIF14 as a regulator of cilia formation and Hedgehog (HH) signaling. We show that RNAi depletion of KIF14 specifically leads to defects in ciliogenesis and basal body (BB) biogenesis, as its absence hampers the efficiency of primary cilium formation and the dynamics of primary cilium elongation, and disrupts the localization of the distal appendage proteins SCLT1 and FBF1 and components of the IFT-B complex. We identify deregulated Aurora A activity as a mechanism contributing to the primary cilium and BB formation defects seen after KIF14 depletion. In addition, we show that primary cilia in KIF14-depleted cells are defective in response to HH pathway activation, independently of the effects of Aurora A. In sum, our data point to KIF14 as a critical node connecting cell cycle machinery, effective ciliogenesis, and HH signaling.
ER  -

PEJŠKOVÁ, Petra, Madeline Louise REILLY, Lucia BINÓ, Ondřej BERNATÍK, Linda DOLANSKÁ, Sri Ranjani GANJI, Zbyněk ZDRÁHAL, Alexandre BENMERAH and Lukáš ČAJÁNEK. KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling. \textit{JOURNAL OF CELL BIOLOGY}. New York: ROCKEFELLER UNIVERSITY PRESS, 2020, vol.~219, No~6, p.~''1-23''-''S1-S6'', 29 pp. ISSN~0021-9525. Available from: https://dx.doi.org/10.1083/jcb.201904107.

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