J 2020

KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling

PEJŠKOVÁ, Petra, Madeline Louise REILLY, Lucia BINÓ, Ondřej BERNATÍK, Linda DOLANSKÁ et. al.

Basic information

Original name

KIF14 controls ciliogenesis via regulation of Aurora A and is important for Hedgehog signaling

Authors

PEJŠKOVÁ, Petra (203 Czech Republic, belonging to the institution), Madeline Louise REILLY (250 France), Lucia BINÓ (703 Slovakia, belonging to the institution), Ondřej BERNATÍK (203 Czech Republic, belonging to the institution), Linda DOLANSKÁ (203 Czech Republic, belonging to the institution), Sri Ranjani GANJI (356 India), Zbyněk ZDRÁHAL (203 Czech Republic), Alexandre BENMERAH (250 France) and Lukáš ČAJÁNEK (203 Czech Republic, guarantor, belonging to the institution)

Edition

JOURNAL OF CELL BIOLOGY, New York, ROCKEFELLER UNIVERSITY PRESS, 2020, 0021-9525

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 10.539

RIV identification code

RIV/00216224:14110/20:00114130

Organization unit

Faculty of Medicine

DOI

UT WoS

000538141100021

Keywords in English

PRIMARY CILIUM; MOTHER CENTRIOLE; MOTOR PROTEINS; SONIC HEDGEHOG; EARLY STEPS; KINESIN-II; CELL-CYCLE; A KINASE; ACTIVATION; CEP164

Tags

Tags

International impact, Reviewed
Změněno: 10/10/2024 13:52, Ing. Martina Blahová

Abstract

V originále

Primary cilia play critical roles in development and disease. Their assembly and disassembly are tightly coupled to cell cycle progression. Here, we present data identifying KIF14 as a regulator of cilia formation and Hedgehog (HH) signaling. We show that RNAi depletion of KIF14 specifically leads to defects in ciliogenesis and basal body (BB) biogenesis, as its absence hampers the efficiency of primary cilium formation and the dynamics of primary cilium elongation, and disrupts the localization of the distal appendage proteins SCLT1 and FBF1 and components of the IFT-B complex. We identify deregulated Aurora A activity as a mechanism contributing to the primary cilium and BB formation defects seen after KIF14 depletion. In addition, we show that primary cilia in KIF14-depleted cells are defective in response to HH pathway activation, independently of the effects of Aurora A. In sum, our data point to KIF14 as a critical node connecting cell cycle machinery, effective ciliogenesis, and HH signaling.

Links

GA19-05244S, research and development project
Name: Molekulární aspekty ciliogeneze: zaměření na Tau Tubulin Kinázu 2
Investor: Czech Science Foundation
GJ16-03269Y, research and development project
Name: Tau tubulin kináza 2 v ciliogenezi: molekulární mechanismy a funkční důsledky
Investor: Czech Science Foundation
LM2018127, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
90127, large research infrastructures
Name: CIISB II
90129, large research infrastructures
Name: Czech-BioImaging II