Application of an electrochemical LAMP-based assay for
screening of HPV16/HPV18 infection in cervical samples

J 2020

Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

ANTON, Milan, Ludmila MORÁŇOVÁ, Roman HRSTKA and Martin BARTOSIK

Basic information

Original name

Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Authors

ANTON, Milan (203 Czech Republic, belonging to the institution), Ludmila MORÁŇOVÁ (203 Czech Republic), Roman HRSTKA (203 Czech Republic) and Martin BARTOSIK (203 Czech Republic, guarantor)

Edition

Analytical Methods, CAMBRIDGE, Royal Society of Chemistry, 2020, 1759-9660

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10401 Organic chemistry

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.896

RIV identification code

RIV/00216224:14110/20:00115637

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1039/c9ay02383f

UT WoS

000514662700008

Keywords in English

HUMAN-PAPILLOMAVIRUS; HPV TEST; RISK; PLATFORM; ATHENA; ACIDS

Abstract

V originále

Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPVs), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and could be implemented in low-resource settings. In this work, we show an early application of an electrochemical (EC) assay combined with loop-mediated amplification (LAMP) to determine HPV16 and HPV18 status directly in a clinical material. To demonstrate its potential usefulness, we screened 61 cervical samples for the presence of both genotypes, and compared the results with the gold standard - PCR amplification followed by gel electrophoresis and two commercial HPV tests - COBAS and INNO-LiPA. The best concordance was obtained with the PCR reaction; we obtained high significance (p-value <0.0001) and positive and negative predictive values over 90%. These data indicate that the EC-LAMP assay may serve as an interesting tool for rapid screening of oncogenic HPVs.

Citovat

ANTON, Milan, Ludmila MORÁŇOVÁ, Roman HRSTKA and Martin BARTOSIK. Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples. Analytical Methods. CAMBRIDGE: Royal Society of Chemistry, 2020, vol. 12, No 6, p. 822-829. ISSN 1759-9660. Available from: https://dx.doi.org/10.1039/c9ay02383f.

@article{1655397,
   author = {Anton, Milan and Moráňová, Ludmila and Hrstka, Roman and Bartosik, Martin},
   article_location = {CAMBRIDGE},
   article_number = {6},
   doi = {http://dx.doi.org/10.1039/c9ay02383f},
   keywords = {HUMAN-PAPILLOMAVIRUS; HPV TEST; RISK; PLATFORM; ATHENA; ACIDS},
   language = {eng},
   issn = {1759-9660},
   journal = {Analytical Methods},
   title = {Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples},
   url = {https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract},
   volume = {12},
   year = {2020}
}

TY  - JOUR
ID  - 1655397
AU  - Anton, Milan - Moráňová, Ludmila - Hrstka, Roman - Bartosik, Martin
PY  - 2020
TI  - Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples
JF  - Analytical Methods
VL  - 12
IS  - 6
SP  - 822-829
EP  - 822-829
PB  - Royal Society of Chemistry
SN  - 17599660
KW  - HUMAN-PAPILLOMAVIRUS
KW  - HPV TEST
KW  - RISK
KW  - PLATFORM
KW  - ATHENA
KW  - ACIDS
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract
L2  - https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract
N2  - Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPVs), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and could be implemented in low-resource settings. In this work, we show an early application of an electrochemical (EC) assay combined with loop-mediated amplification (LAMP) to determine HPV16 and HPV18 status directly in a clinical material. To demonstrate its potential usefulness, we screened 61 cervical samples for the presence of both genotypes, and compared the results with the gold standard - PCR amplification followed by gel electrophoresis and two commercial HPV tests - COBAS and INNO-LiPA. The best concordance was obtained with the PCR reaction; we obtained high significance (p-value <0.0001) and positive and negative predictive values over 90%. These data indicate that the EC-LAMP assay may serve as an interesting tool for rapid screening of oncogenic HPVs.
ER  -

ANTON, Milan, Ludmila MORÁŇOVÁ, Roman HRSTKA and Martin BARTOSIK. Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples. \textit{Analytical Methods}. CAMBRIDGE: Royal Society of Chemistry, 2020, vol.~12, No~6, p.~822-829. ISSN~1759-9660. Available from: https://dx.doi.org/10.1039/c9ay02383f.

Detailed Information on Publication Record