Genes regulating hormone stimulus and response to protein
signaling revealed differential expression pattern during
porcine oocyte in vitro maturation, confirmed by lipid
concentration

J 2020

Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration

CHERMULA, Blazej, Michal JEŠETA, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Maurycy JANKOWSKI et. al.

Basic information

Original name

Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration

Authors

CHERMULA, Blazej (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Patrycja SUJKA-KORDOWSKA (616 Poland), Aneta KONWERSKA (616 Poland), Maurycy JANKOWSKI (616 Poland), Wieslawa KRANC (616 Poland), Ievgeniia KOCHEROVA (616 Poland), Piotr CELICHOWSKI (616 Poland), Piotr ANTOSIK (616 Poland), Dorota BUKOWSKA (616 Poland), Irena MILAKOVIC (203 Czech Republic), Marie MACHATKOVA (203 Czech Republic), Leszek PAWELCZYK (616 Poland), Dariusz IZYCKI (616 Poland), Maciej ZABEL (616 Poland), Paul MOZDZIAK (840 United States of America), Bartosz KEMPISTY (616 Poland, guarantor) and Hanna PIOTROWSKA-KEMPISTY (616 Poland)

Edition

Histochemistry and Cell Biology, New York, Springer, 2020, 0948-6143

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.304

RIV identification code

RIV/00216224:14110/20:00115638

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00418-020-01866-w

UT WoS

000520818700001

Keywords in English

Pig; Oocyte maturation; Microarray; Mitochondrial activity

Abstract

V originále

Genes influencing oocyte maturation may be valuable for predicting their developmental potential, as well as discerning the mechanistic pathways regulating oocyte development. In the presented research microarray gene expression analysis of immature and in vitro matured porcine oocytes was performed. Two groups of oocytes were compared in the study: before (3 x n = 50) and after in vitro maturation (3 x n = 50). The selection of viable oocytes was performed using the brilliant cresyl blue (BCB) test. Furthermore, microarrays and RT-qPCR was used to analyze the transcriptome of the oocytes before and after IVM. The study focused on the genes undergoing differential expression in two gene-ontology groups: "Cellular response to hormone stimulus" and "Cellular response to unfolded protein", which contain genes that may directly or indirectly be involved in signal transduction during oocyte maturation. Examination of all the genes of interest showed a lower level of their expression after IVM. From the total number of genes in these gene ontologies ten of the highest change in expression were identified: FOS, ID2, BTG2, CYR61, ESR1, AR, TACR3, CCND2, EGR2 and TGFBR3. The successful maturation of the oocytes was additionally confirmed with the use of lipid droplet assay. The genes were briefly described and related to the literature sources, to investigate their potential roles in the process of oocyte maturation. The results of the study may serve as a basic molecular reference for further research aimed at improving the methods of oocyte in vitro maturation, which plays an important role in the procedures of assisted reproduction.

Citovat

CHERMULA, Blazej, Michal JEŠETA, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Maurycy JANKOWSKI, Wieslawa KRANC, Ievgeniia KOCHEROVA, Piotr CELICHOWSKI, Piotr ANTOSIK, Dorota BUKOWSKA, Irena MILAKOVIC, Marie MACHATKOVA, Leszek PAWELCZYK, Dariusz IZYCKI, Maciej ZABEL, Paul MOZDZIAK, Bartosz KEMPISTY and Hanna PIOTROWSKA-KEMPISTY. Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration. Histochemistry and Cell Biology. New York: Springer, 2020, vol. 154, No 1, p. 77-95. ISSN 0948-6143. Available from: https://dx.doi.org/10.1007/s00418-020-01866-w.

@article{1655416,
   author = {Chermula, Blazej and Ješeta, Michal and SujkaandKordowska, Patrycja and Konwerska, Aneta and Jankowski, Maurycy and Kranc, Wieslawa and Kocherova, Ievgeniia and Celichowski, Piotr and Antosik, Piotr and Bukowska, Dorota and Milakovic, Irena and Machatkova, Marie and Pawelczyk, Leszek and Izycki, Dariusz and Zabel, Maciej and Mozdziak, Paul and Kempisty, Bartosz and PiotrowskaandKempisty, Hanna},
   article_location = {New York},
   article_number = {1},
   doi = {http://dx.doi.org/10.1007/s00418-020-01866-w},
   keywords = {Pig; Oocyte maturation; Microarray; Mitochondrial activity},
   language = {eng},
   issn = {0948-6143},
   journal = {Histochemistry and Cell Biology},
   title = {Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration},
   url = {https://link.springer.com/article/10.1007/s00418-020-01866-w},
   volume = {154},
   year = {2020}
}

TY  - JOUR
ID  - 1655416
AU  - Chermula, Blazej - Ješeta, Michal - Sujka-Kordowska, Patrycja - Konwerska, Aneta - Jankowski, Maurycy - Kranc, Wieslawa - Kocherova, Ievgeniia - Celichowski, Piotr - Antosik, Piotr - Bukowska, Dorota - Milakovic, Irena - Machatkova, Marie - Pawelczyk, Leszek - Izycki, Dariusz - Zabel, Maciej - Mozdziak, Paul - Kempisty, Bartosz - Piotrowska-Kempisty, Hanna
PY  - 2020
TI  - Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration
JF  - Histochemistry and Cell Biology
VL  - 154
IS  - 1
SP  - 77-95
EP  - 77-95
PB  - Springer
SN  - 09486143
KW  - Pig
KW  - Oocyte maturation
KW  - Microarray
KW  - Mitochondrial activity
UR  - https://link.springer.com/article/10.1007/s00418-020-01866-w
L2  - https://link.springer.com/article/10.1007/s00418-020-01866-w
N2  - Genes influencing oocyte maturation may be valuable for predicting their developmental potential, as well as discerning the mechanistic pathways regulating oocyte development. In the presented research microarray gene expression analysis of immature and in vitro matured porcine oocytes was performed. Two groups of oocytes were compared in the study: before (3 x n = 50) and after in vitro maturation (3 x n = 50). The selection of viable oocytes was performed using the brilliant cresyl blue (BCB) test. Furthermore, microarrays and RT-qPCR was used to analyze the transcriptome of the oocytes before and after IVM. The study focused on the genes undergoing differential expression in two gene-ontology groups: "Cellular response to hormone stimulus" and "Cellular response to unfolded protein", which contain genes that may directly or indirectly be involved in signal transduction during oocyte maturation. Examination of all the genes of interest showed a lower level of their expression after IVM. From the total number of genes in these gene ontologies ten of the highest change in expression were identified: FOS, ID2, BTG2, CYR61, ESR1, AR, TACR3, CCND2, EGR2 and TGFBR3. The successful maturation of the oocytes was additionally confirmed with the use of lipid droplet assay. The genes were briefly described and related to the literature sources, to investigate their potential roles in the process of oocyte maturation. The results of the study may serve as a basic molecular reference for further research aimed at improving the methods of oocyte in vitro maturation, which plays an important role in the procedures of assisted reproduction.
ER  -

CHERMULA, Blazej, Michal JEŠETA, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Maurycy JANKOWSKI, Wieslawa KRANC, Ievgeniia KOCHEROVA, Piotr CELICHOWSKI, Piotr ANTOSIK, Dorota BUKOWSKA, Irena MILAKOVIC, Marie MACHATKOVA, Leszek PAWELCZYK, Dariusz IZYCKI, Maciej ZABEL, Paul MOZDZIAK, Bartosz KEMPISTY and Hanna PIOTROWSKA-KEMPISTY. Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration. \textit{Histochemistry and Cell Biology}. New York: Springer, 2020, vol.~154, No~1, p.~77-95. ISSN~0948-6143. Available from: https://dx.doi.org/10.1007/s00418-020-01866-w.

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