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@article{1655416,
author = {Chermula, Blazej and Ješeta, Michal and SujkaandKordowska, Patrycja and Konwerska, Aneta and Jankowski, Maurycy and Kranc, Wieslawa and Kocherova, Ievgeniia and Celichowski, Piotr and Antosik, Piotr and Bukowska, Dorota and Milakovic, Irena and Machatkova, Marie and Pawelczyk, Leszek and Izycki, Dariusz and Zabel, Maciej and Mozdziak, Paul and Kempisty, Bartosz and PiotrowskaandKempisty, Hanna},
article_location = {New York},
article_number = {1},
doi = {http://dx.doi.org/10.1007/s00418-020-01866-w},
keywords = {Pig; Oocyte maturation; Microarray; Mitochondrial activity},
language = {eng},
issn = {0948-6143},
journal = {Histochemistry and Cell Biology},
title = {Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration},
url = {https://link.springer.com/article/10.1007/s00418-020-01866-w},
volume = {154},
year = {2020}
}
TY - JOUR
ID - 1655416
AU - Chermula, Blazej - Ješeta, Michal - Sujka-Kordowska, Patrycja - Konwerska, Aneta - Jankowski, Maurycy - Kranc, Wieslawa - Kocherova, Ievgeniia - Celichowski, Piotr - Antosik, Piotr - Bukowska, Dorota - Milakovic, Irena - Machatkova, Marie - Pawelczyk, Leszek - Izycki, Dariusz - Zabel, Maciej - Mozdziak, Paul - Kempisty, Bartosz - Piotrowska-Kempisty, Hanna
PY - 2020
TI - Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration
JF - Histochemistry and Cell Biology
VL - 154
IS - 1
SP - 77-95
EP - 77-95
PB - Springer
SN - 09486143
KW - Pig
KW - Oocyte maturation
KW - Microarray
KW - Mitochondrial activity
UR - https://link.springer.com/article/10.1007/s00418-020-01866-w
L2 - https://link.springer.com/article/10.1007/s00418-020-01866-w
N2 - Genes influencing oocyte maturation may be valuable for predicting their developmental potential, as well as discerning the mechanistic pathways regulating oocyte development. In the presented research microarray gene expression analysis of immature and in vitro matured porcine oocytes was performed. Two groups of oocytes were compared in the study: before (3 x n = 50) and after in vitro maturation (3 x n = 50). The selection of viable oocytes was performed using the brilliant cresyl blue (BCB) test. Furthermore, microarrays and RT-qPCR was used to analyze the transcriptome of the oocytes before and after IVM. The study focused on the genes undergoing differential expression in two gene-ontology groups: "Cellular response to hormone stimulus" and "Cellular response to unfolded protein", which contain genes that may directly or indirectly be involved in signal transduction during oocyte maturation. Examination of all the genes of interest showed a lower level of their expression after IVM. From the total number of genes in these gene ontologies ten of the highest change in expression were identified: FOS, ID2, BTG2, CYR61, ESR1, AR, TACR3, CCND2, EGR2 and TGFBR3. The successful maturation of the oocytes was additionally confirmed with the use of lipid droplet assay. The genes were briefly described and related to the literature sources, to investigate their potential roles in the process of oocyte maturation. The results of the study may serve as a basic molecular reference for further research aimed at improving the methods of oocyte in vitro maturation, which plays an important role in the procedures of assisted reproduction.
ER -
CHERMULA, Blazej, Michal JEŠETA, Patrycja SUJKA-KORDOWSKA, Aneta KONWERSKA, Maurycy JANKOWSKI, Wieslawa KRANC, Ievgeniia KOCHEROVA, Piotr CELICHOWSKI, Piotr ANTOSIK, Dorota BUKOWSKA, Irena MILAKOVIC, Marie MACHATKOVA, Leszek PAWELCZYK, Dariusz IZYCKI, Maciej ZABEL, Paul MOZDZIAK, Bartosz KEMPISTY and Hanna PIOTROWSKA-KEMPISTY. Genes regulating hormone stimulus and response to protein signaling revealed differential expression pattern during porcine oocyte in vitro maturation, confirmed by lipid concentration. \textit{Histochemistry and Cell Biology}. New York: Springer, 2020, vol.~154, No~1, p.~77-95. ISSN~0948-6143. Available from: https://dx.doi.org/10.1007/s00418-020-01866-w.
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