Quantitative Phase Dynamics of Cancer Cell Populations Affected
by Blue Light

J 2020

Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

FEITH, Marek, Tomáš VIČAR, Jaromír GUMULEC, Martina RAUDENSKÁ, Anette GJÖRLOFF WINGREN et. al.

Basic information

Original name

Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

Authors

FEITH, Marek (203 Czech Republic, belonging to the institution), Tomáš VIČAR (203 Czech Republic, belonging to the institution), Jaromír GUMULEC (203 Czech Republic), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Anette GJÖRLOFF WINGREN (752 Sweden), Michal MASAŘÍK (203 Czech Republic, belonging to the institution) and Jan BALVAN (203 Czech Republic, guarantor, belonging to the institution)

Edition

Applied Sciences, Basel, MDPI, 2020, 2076-3417

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10301 Atomic, molecular and chemical physics

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.679

RIV identification code

RIV/00216224:14110/20:00114154

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/app10072597

UT WoS

000533356200396

Keywords in English

holographic microscopy; quantitative phase imaging; blue light; cell mass; cell motility; cell death

Abstract

V originále

Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm(2), 208 mJ/cm(2) and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm(2) x 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm(2) x 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.

Links

GA18-24089S, research and development project

Name: Kvantitativní fázová mikroskopie pro 3D kvalitativní charakterizaci nádorových buněk

Investor: Czech Science Foundation

ROZV/28/LF24/2020, interní kód MU

Name: Analýza kvantitativně-fázové dynamiky lytických typů buněčné smrti

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

FEITH, Marek, Tomáš VIČAR, Jaromír GUMULEC, Martina RAUDENSKÁ, Anette GJÖRLOFF WINGREN, Michal MASAŘÍK and Jan BALVAN. Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light. Applied Sciences. Basel: MDPI, 2020, vol. 10, No 7, p. 1-13. ISSN 2076-3417. Available from: https://dx.doi.org/10.3390/app10072597.

@article{1658657,
   author = {Feith, Marek and Vičar, Tomáš and Gumulec, Jaromír and Raudenská, Martina and Gjörloff Wingren, Anette and Masařík, Michal and Balvan, Jan},
   article_location = {Basel},
   article_number = {7},
   doi = {http://dx.doi.org/10.3390/app10072597},
   keywords = {holographic microscopy; quantitative phase imaging; blue light; cell mass; cell motility; cell death},
   language = {eng},
   issn = {2076-3417},
   journal = {Applied Sciences},
   title = {Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light},
   url = {https://www.mdpi.com/2076-3417/10/7/2597},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1658657
AU  - Feith, Marek - Vičar, Tomáš - Gumulec, Jaromír - Raudenská, Martina - Gjörloff Wingren, Anette - Masařík, Michal - Balvan, Jan
PY  - 2020
TI  - Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light
JF  - Applied Sciences
VL  - 10
IS  - 7
SP  - 1-13
EP  - 1-13
PB  - MDPI
SN  - 20763417
KW  - holographic microscopy
KW  - quantitative phase imaging
KW  - blue light
KW  - cell mass
KW  - cell motility
KW  - cell death
UR  - https://www.mdpi.com/2076-3417/10/7/2597
L2  - https://www.mdpi.com/2076-3417/10/7/2597
N2  - Increased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm(2), 208 mJ/cm(2) and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm(2) x 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm(2) x 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.
ER  -

FEITH, Marek, Tomáš VIČAR, Jaromír GUMULEC, Martina RAUDENSKÁ, Anette GJÖRLOFF WINGREN, Michal MASAŘÍK and Jan BALVAN. Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light. \textit{Applied Sciences}. Basel: MDPI, 2020, vol.~10, No~7, p.~1-13. ISSN~2076-3417. Available from: https://dx.doi.org/10.3390/app10072597.

Detailed Information on Publication Record