J 2020

Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection

ELIÁŠ, Sara, Jana HURYCHOVÁ, Duarte Nuno TOUBARRO TIAGO, Jorge FRIAS, Martin KUNC et. al.

Basic information

Original name

Bioactive Excreted/Secreted Products of Entomopathogenic Nematode Heterorhabditis bacteriophora Inhibit the Phenoloxidase Activity during the Infection

Authors

ELIÁŠ, Sara (276 Germany, belonging to the institution), Jana HURYCHOVÁ (203 Czech Republic, belonging to the institution), Duarte Nuno TOUBARRO TIAGO (620 Portugal), Jorge FRIAS (620 Portugal), Martin KUNC (203 Czech Republic, belonging to the institution), Pavel DOBEŠ (203 Czech Republic, belonging to the institution), Nelson SIMÕES (620 Portugal) and Pavel HYRŠL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Insects, Basel, MDPI, 2020, 2075-4450

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.769

RIV identification code

RIV/00216224:14310/20:00114166

Organization unit

Faculty of Science

DOI

UT WoS

000553609400001

Keywords (in Czech)

Heterorhabditis bacteriophora; exkretované/sekretované produkty; fenoloxidáza; imunita; melanizace; Galleria mellonella; virulence

Keywords in English

Heterorhabditis bacteriophora; excreted/secreted products; phenoloxidase; immunity; melanization; Galleria mellonella; virulence

Tags

Tags

International impact, Reviewed
Změněno: 21/10/2020 16:39, Mgr. Marie Šípková, DiS.

Abstract

V originále

Entomopathogenic nematodes (EPNs) are efficient insect parasites, that are known for their mutualistic relationship with entomopathogenic bacteria and their use in biocontrol. EPNs produce bioactive molecules referred to as excreted/secreted products (ESPs), which have come to the forefront in recent years because of their role in the process of host invasion and the modulation of its immune response. In the present study, we confirmed the production of ESPs in the EPN Heterorhabditis bacteriophora, and investigated their role in the modulation of the phenoloxidase cascade, one of the key components of the insect immune system. ESPs were isolated from 14- and 21-day-old infective juveniles of H. bacteriophora, which were found to be more virulent than newly emerged nematodes, as was confirmed by mortality assays using Galleria mellonella larvae. The isolated ESPs were further purified and screened for the phenoloxidase-inhibiting activity. In these products, a 38 kDa fraction of peptides was identified as the main candidate source of phenoloxidase-inhibiting compounds. This fraction was further analyzed by mass spectrometry and the de novo sequencing approach. Six peptide sequences were identified in this active ESP fraction, including proteins involved in ubiquitination and the regulation of a Toll pathway, for which a role in the regulation of insect immune response has been proposed in previous studies.

Links

GA17-03253S, research and development project
Name: Hormonální kontrola hmyzího obranného systému
Investor: Czech Science Foundation