Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA
Polymerase II C-Terminal Domain In Vitro

J 2020

Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro

BRÁZDA, Pavel, Magdaléna KREJČÍKOVÁ, Aiste KASILIAUSKAITE, Eliška ŠMIŘÁKOVÁ, Tomáš KLUMPLER et. al.

Basic information

Original name

Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro

Authors

BRÁZDA, Pavel (203 Czech Republic, belonging to the institution), Magdaléna KREJČÍKOVÁ (203 Czech Republic, belonging to the institution), Aiste KASILIAUSKAITE (440 Lithuania, belonging to the institution), Eliška ŠMIŘÁKOVÁ (203 Czech Republic, belonging to the institution), Tomáš KLUMPLER (203 Czech Republic, belonging to the institution), Robert VÁCHA (203 Czech Republic, belonging to the institution), Karel KUBÍČEK (203 Czech Republic, belonging to the institution) and Richard ŠTEFL (203 Czech Republic, belonging to the institution)

Edition

Journal of Molecular Biology, Academic Press, 2020, 0022-2836

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.469

RIV identification code

RIV/00216224:14740/20:00114180

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.jmb.2020.05.007

UT WoS

000543017000010

Keywords in English

CTD; RNA polymerase II; Spt6; NMR structure; phosphorylation

Abstract

V originále

Transcription elongation factor Spt6 associates with RNA polymerase II (RNAP II) via a tandem SH2 (tSH2) domain. The mechanism and significance of the RNAP II-Spt6 interaction is still unclear. Recently, it was proposed that Spt6-tSH2 is recruited via a newly described phosphorylated linker between the Rpb1 core and its C-terminal domain (CTD). Here, we report binding studies with isolated tSH2 of Spt6 (Spt6-tSH2) and Spt6 lacking the first unstructured 297 residues (Spt6N) with a minimal CTD substrate of two repetitive heptads phosphorylated at different sites. The data demonstrate that Spt6 also binds the phosphorylated CTD, a site that was originally proposed as a recognition epitope. We also show that an extended CTD substrate harboring 13 repetitive heptads of the tyrosine-phosphorylated CTD binds Spt6-tSH2 and Spt6N with tighter affinity than the minimal CTD substrate. The enhanced binding is achieved by avidity originating from multiple phosphorylation marks present in the CTD. Interestingly, we found that the steric effects of additional domains in the Spt6N construct partially obscure the binding of the tSH2 domain to the multivalent ligand. We show that Spt6-tSH2 binds various phosphorylation patterns in the CTD and found that the studied combinations of phospho-CTD marks (1,2; 1,5; 2,4; and 2,7) all facilitate the interaction of CTD with Spt6. Our structural studies reveal a plasticity of the tSH2 binding pockets that enables the accommodation of CTDs with phosphorylation marks in different registers.

Links

GA18-11397S, research and development project

Name: Molekulární achitektura komplexů ADAR - RNA

Investor: Czech Science Foundation

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/G/1100/2016, interní kód MU

Name: Computational chemistry for Wnt signaling pathway

Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects

649030, interní kód MU

Name: DECOR - Dynamic assembly and exchange of RNA polymerase II CTD factors (Acronym: DECOR)

Investor: European Union, DECOR, ERC (Excellent Science)

90127, large research infrastructures

Name: CIISB II

Citovat

BRÁZDA, Pavel, Magdaléna KREJČÍKOVÁ, Aiste KASILIAUSKAITE, Eliška ŠMIŘÁKOVÁ, Tomáš KLUMPLER, Robert VÁCHA, Karel KUBÍČEK and Richard ŠTEFL. Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro. Journal of Molecular Biology. Academic Press, 2020, vol. 432, No 14, p. 4092-4107. ISSN 0022-2836. Available from: https://dx.doi.org/10.1016/j.jmb.2020.05.007.

@article{1662603,
   author = {Brázda, Pavel and Krejčíková, Magdaléna and Kasiliauskaite, Aiste and Šmiřáková, Eliška and Klumpler, Tomáš and Vácha, Robert and Kubíček, Karel and Štefl, Richard},
   article_number = {14},
   doi = {http://dx.doi.org/10.1016/j.jmb.2020.05.007},
   keywords = {CTD; RNA polymerase II; Spt6; NMR structure; phosphorylation},
   language = {eng},
   issn = {0022-2836},
   journal = {Journal of Molecular Biology},
   title = {Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro},
   url = {https://www.sciencedirect.com/science/article/pii/S002228362030348X?via%3Dihub},
   volume = {432},
   year = {2020}
}

TY  - JOUR
ID  - 1662603
AU  - Brázda, Pavel - Krejčíková, Magdaléna - Kasiliauskaite, Aiste - Šmiřáková, Eliška - Klumpler, Tomáš - Vácha, Robert - Kubíček, Karel - Štefl, Richard
PY  - 2020
TI  - Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro
JF  - Journal of Molecular Biology
VL  - 432
IS  - 14
SP  - 4092-4107
EP  - 4092-4107
PB  - Academic Press
SN  - 00222836
KW  - CTD
KW  - RNA polymerase II
KW  - Spt6
KW  - NMR structure
KW  - phosphorylation
UR  - https://www.sciencedirect.com/science/article/pii/S002228362030348X?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S002228362030348X?via%3Dihub
N2  - Transcription elongation factor Spt6 associates with RNA polymerase II (RNAP II) via a tandem SH2 (tSH2) domain. The mechanism and significance of the RNAP II-Spt6 interaction is still unclear. Recently, it was proposed that Spt6-tSH2 is recruited via a newly described phosphorylated linker between the Rpb1 core and its C-terminal domain (CTD). Here, we report binding studies with isolated tSH2 of Spt6 (Spt6-tSH2) and Spt6 lacking the first unstructured 297 residues (Spt6N) with a minimal CTD substrate of two repetitive heptads phosphorylated at different sites. The data demonstrate that Spt6 also binds the phosphorylated CTD, a site that was originally proposed as a recognition epitope. We also show that an extended CTD substrate harboring 13 repetitive heptads of the tyrosine-phosphorylated CTD binds Spt6-tSH2 and Spt6N with tighter affinity than the minimal CTD substrate. The enhanced binding is achieved by avidity originating from multiple phosphorylation marks present in the CTD. Interestingly, we found that the steric effects of additional domains in the Spt6N construct partially obscure the binding of the tSH2 domain to the multivalent ligand. We show that Spt6-tSH2 binds various phosphorylation patterns in the CTD and found that the studied combinations of phospho-CTD marks (1,2; 1,5; 2,4; and 2,7) all facilitate the interaction of CTD with Spt6. Our structural studies reveal a plasticity of the tSH2 binding pockets that enables the accommodation of CTDs with phosphorylation marks in different registers.
ER  -

BRÁZDA, Pavel, Magdaléna KREJČÍKOVÁ, Aiste KASILIAUSKAITE, Eliška ŠMIŘÁKOVÁ, Tomáš KLUMPLER, Robert VÁCHA, Karel KUBÍČEK and Richard ŠTEFL. Yeast Spt6 Reads Multiple Phosphorylation Patterns of RNA Polymerase II C-Terminal Domain In Vitro. \textit{Journal of Molecular Biology}. Academic Press, 2020, vol.~432, No~14, p.~4092-4107. ISSN~0022-2836. Available from: https://dx.doi.org/10.1016/j.jmb.2020.05.007.

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