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@article{1663937,
author = {Heger, Tomáš and Zukal, Jan and Seidlová, Veronika and Němcová, Monika and Nečas, David and Papežíková, Ivana and Piaček, Vladimír and Zajíčková, Renata and Banďouchová, Hana and Pikula, Jiří},
article_location = {Brno},
article_number = {1},
doi = {http://dx.doi.org/10.2754/avb202089010079},
keywords = {Innate immunity; respiratory burst; torpor; hibernation; bat; laboratory mouse},
language = {eng},
issn = {0001-7213},
journal = {Acta Veterinaria Brno},
title = {Measurement of phagocyte activity in heterotherms},
url = {https://doi.org/10.2754/avb202089010079},
volume = {89},
year = {2020}
}
TY - JOUR
ID - 1663937
AU - Heger, Tomáš - Zukal, Jan - Seidlová, Veronika - Němcová, Monika - Nečas, David - Papežíková, Ivana - Piaček, Vladimír - Zajíčková, Renata - Banďouchová, Hana - Pikula, Jiří
PY - 2020
TI - Measurement of phagocyte activity in heterotherms
JF - Acta Veterinaria Brno
VL - 89
IS - 1
SP - 79-87
EP - 79-87
PB - University of Veterinary and Pharmaceutical Sciences in Brno
SN - 00017213
KW - Innate immunity
KW - respiratory burst
KW - torpor
KW - hibernation
KW - bat
KW - laboratory mouse
UR - https://doi.org/10.2754/avb202089010079
L2 - https://doi.org/10.2754/avb202089010079
N2 - The heterotherm immune system undergoes significant variation in response to life cycle periodicity and torpor. As heterothermic bats are important reservoirs of zoonotic agents and modulation of immune activity can affect host-pathogen interactions, this work aimed at developing a suitable method for assessing heterotherm phagocyte activity. Chemiluminescence measurements were evaluated by mathematical and mechanistic approaches, both of which yielded comparable results in time-related parameters of phagocyte activity. Using a mathematical method, however, we developed a model that can be applied to particular specimens. The proposed equation offers a simple and reliable tool for comparing phagocyte activity, the values of which can be used for further analysis. While time-related parameters of bat phagocyte activity varied with measurement temperature, with the onset of respiratory burst at 38 degrees C being quicker than at 25 degrees C, quantitative values of phagocyte activity were not influenced by measurement temperature. Further, homeotherm phagocyte activity parameters were more variable at 25 degrees C. Considering there was no influence of measurement temperature on the total volume of heterotherm phagocyte activity, we suggest that parameters measured at 25 degrees C are more representative of the immune status adapted to physiological extremes at low body temperatures.
ER -
HEGER, Tomáš, Jan ZUKAL, Veronika SEIDLOVÁ, Monika NĚMCOVÁ, David NEČAS, Ivana PAPEŽÍKOVÁ, Vladimír PIAČEK, Renata ZAJÍČKOVÁ, Hana BANĎOUCHOVÁ a Jiří PIKULA. Measurement of phagocyte activity in heterotherms. \textit{Acta Veterinaria Brno}. Brno: University of Veterinary and Pharmaceutical Sciences in Brno, 2020, roč.~89, č.~1, s.~79-87. ISSN~0001-7213. Dostupné z: https://dx.doi.org/10.2754/avb202089010079.
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