OREKHOVA, Alla, Miloš BARTÁK and Romana GAŠPAROVÁ. Morphometric Analysis of Antarctic Foliose Lichens Using 3-D Digital Microscopy. Online. In Abstract Proceedings Open Science Conference 19 – 23 June 2018 Davos, Switzerland. Davos: WSL Institute for Snow and Avalanche Research SLF, 2018, p. 1530-1530. ISBN 978-0-948277-54-2.
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Basic information
Original name Morphometric Analysis of Antarctic Foliose Lichens Using 3-D Digital Microscopy
Name in Czech Morfometrická analýza antarktických foliózních lišejníků s využitím 3-D digitální mikroskopie
Name (in English) Morphometric Analysis of Antarctic Foliose Lichens Using 3-D Digital Microscopy
Authors OREKHOVA, Alla, Miloš BARTÁK and Romana GAŠPAROVÁ.
Edition Davos, Abstract Proceedings Open Science Conference 19 – 23 June 2018 Davos, Switzerland, p. 1530-1530, 1 pp. 2018.
Publisher WSL Institute for Snow and Avalanche Research SLF
Other information
Type of outcome Proceedings paper
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
WWW Conference Abstract book
ISBN 978-0-948277-54-2
Keywords (in Czech) morfologie; stélka; Placopsis contortuplicata; Dermatocarpon polyphillizum; Rhizoplaca melaophthalma
Keywords in English morphology; thallus; Placopsis contortuplicata; Dermatocarpon polyphillizum; Rhizoplaca melaophthalma
Tags International impact
Changed by Changed by: prof. Ing. Miloš Barták, CSc., učo 57. Changed: 23/6/2020 15:29.
Abstract
Recently, digital microscopy is an emerging technique that combines the tools of classic light microscopy with a computerized imaging system. Here we present application of the method in morphometric analysis of Antarctic lichen thalli. The main goal of the study was to evaluate changes in 3-D structute of thalli as dependent on hydration status, and interspecific differences as well. Another goal was to provide quantitative biometrical data on size and shape of morhological structures such as fruiting bodies. Lichen thalli collected from several sites on deglaciated northern part of James Ross Island were analysed using a digital VHX-900F microscope (Keyence, Japan) with a maximum resolution of 19.5 megapixels. The microscope with built-in LEDs allowed accurate measurements of lichen surface structures viewed in real time depth composition at the magnificion ranging from 20 to 200. Data forming a 3-D view of the upper cortex were used to analyze the size of lobes, outgrowths, and fruiting bodies, respectively. Using a VHX software, we applied processing tools: 1) height profile along a selected line placed across a thallus, 2) DFP method (Depth From Defocus) which compiles different focal planes. We studied Placopsis contortuplicata, Dermatocarpon polyphillizum, Rhizoplaca melaophthalma and other species. Gained resuts on a 3-D structure are discussed: interspecific, hydration-, and developmental stage-dependent differences.
Abstract (in Czech)
V poslední době je digitální mikroskopie nová technika, která kombinuje nástroje klasické světelné mikroskopie s počítačovým zobrazovacím systémem. Tady předkládáme aplikaci metody v morfometrické analýze lišejníků antarktických. Hlavním cílem studie bylo vyhodnotit změny ve 3-D struktuře talli v závislosti na stavu hydratace a také mezidruhové rozdíly. Dalším cílem bylo poskytnout kvantitativní biometrické údaje o velikosti a tvaru morologických struktur, jako jsou plodnice. Lichen thalli shromážděný z několika míst na deglaciované severní části ostrova James Ross Island byl analyzován pomocí digitálního mikroskopu VHX-900F (Keyence, Japonsko) s maximálním rozlišením 19,5 megapixelů. Mikroskop s vestavěnými LED diodami umožnil přesné měření struktury povrchů lišejníků při pohledu na složení hloubky v reálném čase při velkoleposti v rozmezí od 20 do 200. K analýze velikosti laloků, výrůstků byly použity údaje tvořící trojrozměrný pohled na horní kůru. , a plodnice, příslušně. Pomocí softwaru VHX jsme použili nástroje pro zpracování: 1) výškový profil podél vybrané linie umístěné přes talu, 2) metodu DFP (Depth From Defocus), která sestavuje různé ohniskové roviny. Studovali jsme Placopsis contortuplicata, Dermatocarpon polyphillizum, Rhizoplaca melaophthalma a další druhy. Jsou diskutovány získané resuty na 3-D struktuře: interspecifické, hydratační a vývojové rozdíly závislé na stadiu.
Abstract (in English)
Recently, digital microscopy is an emerging technique that combines the tools of classic light microscopy with a computerized imaging system. Here we present application of the method in morphometric analysis of Antarctic lichen thalli. The main goal of the study was to evaluate changes in 3-D structute of thalli as dependent on hydration status, and interspecific differences as well. Another goal was to provide quantitative biometrical data on size and shape of morhological structures such as fruiting bodies. Lichen thalli collected from several sites on deglaciated northern part of James Ross Island were analysed using a digital VHX-900F microscope (Keyence, Japan) with a maximum resolution of 19.5 megapixels. The microscope with built-in LEDs allowed accurate measurements of lichen surface structures viewed in real time depth composition at the magnificion ranging from 20 to 200. Data forming a 3-D view of the upper cortex were used to analyze the size of lobes, outgrowths, and fruiting bodies, respectively. Using a VHX software, we applied processing tools: 1) height profile along a selected line placed across a thallus, 2) DFP method (Depth From Defocus) which compiles different focal planes. We studied Placopsis contortuplicata, Dermatocarpon polyphillizum, Rhizoplaca melaophthalma and other species. Gained resuts on a 3-D structure are discussed: interspecific, hydration-, and developmental stage-dependent differences.
Links
CZ.02.1.01/0.0/0.0/16_013/0001708, interní kód MU
(CEP code: EF16_013/0001708)		Name: ECOPOLARIS - Změny ve struktuře a funkci součástí terestrických polárních ekosystémů (Acronym: ECOPOLARIS)
Investor: Ministry of Education, Youth and Sports of the CR, ECOPOLARIS - Changes in structure and function of components of terrestrial polar ecosystems (CzechPolar2), Priority axis 1: Strengthening capacities for high-quality research
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