Advances in Optical Single-Molecule Detection: En Route to
Supersensitive Bioaffinity Assays

J 2020

Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays

FARKA, Zdeněk, Matthias Jürgen MICKERT, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Petr SKLÁDAL et. al.

Basic information

Original name

Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays

Authors

FARKA, Zdeněk (203 Czech Republic, guarantor, belonging to the institution), Matthias Jürgen MICKERT (276 Germany), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Zuzana MIKUŠOVÁ (703 Slovakia, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Hans-Heiner GORRIS (276 Germany)

Edition

Angewandte Chemie International Edition, Weinheim, Wiley-VCH, 2020, 1433-7851

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 15.336

RIV identification code

RIV/00216224:14740/20:00115851

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/anie.201913924

UT WoS

000525812900001

Keywords in English

digital assays; immunoassays; optical detection; signal background; single-molecule detection

Abstract

V originále

The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single-molecule bioaffinity assays. While many review articles have highlighted the potentials of single-molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real-world applications as one should expect. This Review provides a theoretical background on single-molecule—or better digital—assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single-molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.

Links

LTAB19011, research and development project

Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION

MUNI/A/1252/2019, interní kód MU

Name: Podpora biochemického výzkumu v roce 2020

Investor: Masaryk University, Category A

Citovat

FARKA, Zdeněk, Matthias Jürgen MICKERT, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Petr SKLÁDAL and Hans-Heiner GORRIS. Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays. Angewandte Chemie International Edition. Weinheim: Wiley-VCH, 2020, vol. 59, No 27, p. 10746-10773. ISSN 1433-7851. Available from: https://dx.doi.org/10.1002/anie.201913924.

@article{1663988,
   author = {Farka, Zdeněk and Mickert, Matthias Jürgen and Pastucha, Matěj and Mikušová, Zuzana and Skládal, Petr and Gorris, HansandHeiner},
   article_location = {Weinheim},
   article_number = {27},
   doi = {http://dx.doi.org/10.1002/anie.201913924},
   keywords = {digital assays; immunoassays; optical detection; signal background; single-molecule detection},
   language = {eng},
   issn = {1433-7851},
   journal = {Angewandte Chemie International Edition},
   title = {Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/anie.201913924},
   volume = {59},
   year = {2020}
}

TY  - JOUR
ID  - 1663988
AU  - Farka, Zdeněk - Mickert, Matthias Jürgen - Pastucha, Matěj - Mikušová, Zuzana - Skládal, Petr - Gorris, Hans-Heiner
PY  - 2020
TI  - Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays
JF  - Angewandte Chemie International Edition
VL  - 59
IS  - 27
SP  - 10746-10773
EP  - 10746-10773
PB  - Wiley-VCH
SN  - 14337851
KW  - digital assays
KW  - immunoassays
KW  - optical detection
KW  - signal background
KW  - single-molecule detection
UR  - https://onlinelibrary.wiley.com/doi/10.1002/anie.201913924
L2  - https://onlinelibrary.wiley.com/doi/10.1002/anie.201913924
N2  - The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single-molecule bioaffinity assays. While many review articles have highlighted the potentials of single-molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real-world applications as one should expect. This Review provides a theoretical background on single-molecule—or better digital—assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single-molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.
ER  -

FARKA, Zdeněk, Matthias Jürgen MICKERT, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Petr SKLÁDAL and Hans-Heiner GORRIS. Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays. \textit{Angewandte Chemie International Edition}. Weinheim: Wiley-VCH, 2020, vol.~59, No~27, p.~10746-10773. ISSN~1433-7851. Available from: https://dx.doi.org/10.1002/anie.201913924.

Detailed Information on Publication Record