FARKA, Zdeněk, Matthias Jürgen MICKERT, Matěj PASTUCHA, Zuzana MIKUŠOVÁ, Petr SKLÁDAL and Hans-Heiner GORRIS. Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays. Angewandte Chemie International Edition. Weinheim: Wiley-VCH, 2020, vol. 59, No 27, p. 10746-10773. ISSN 1433-7851. Available from: https://dx.doi.org/10.1002/anie.201913924.
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Basic information
Original name Advances in Optical Single-Molecule Detection: En Route to Supersensitive Bioaffinity Assays
Authors FARKA, Zdeněk (203 Czech Republic, guarantor, belonging to the institution), Matthias Jürgen MICKERT (276 Germany), Matěj PASTUCHA (203 Czech Republic, belonging to the institution), Zuzana MIKUŠOVÁ (703 Slovakia, belonging to the institution), Petr SKLÁDAL (203 Czech Republic, belonging to the institution) and Hans-Heiner GORRIS (276 Germany).
Edition Angewandte Chemie International Edition, Weinheim, Wiley-VCH, 2020, 1433-7851.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher Germany
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 15.336
RIV identification code RIV/00216224:14740/20:00115851
Organization unit Central European Institute of Technology
Doi http://dx.doi.org/10.1002/anie.201913924
UT WoS 000525812900001
Keywords in English digital assays; immunoassays; optical detection; signal background; single-molecule detection
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Pavla Foltynová, Ph.D., učo 106624. Changed: 19/2/2021 14:19.
Abstract
The ability to detect low concentrations of analytes and in particular low-abundance biomarkers is of fundamental importance, e.g., for early-stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single-molecule bioaffinity assays. While many review articles have highlighted the potentials of single-molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real-world applications as one should expect. This Review provides a theoretical background on single-molecule—or better digital—assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single-molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.
Links
LTAB19011, research and development projectName: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel
Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION
MUNI/A/1252/2019, interní kód MUName: Podpora biochemického výzkumu v roce 2020
Investor: Masaryk University, Category A
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