| ZEMANOVÁ, Barbora, Petra HAJKOVA, Josef BRYJA, Jan Jr ZIMA, Andrea HAJKOVA and Jan ZIMA. Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois. Folia Zoologica. INST VERTEBRATE BIOLOGY AS CR, 2011, vol. 60, No 1, p. 70-80. ISSN 0139-7893. |
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@article{1664017,
author = {Zemanová, Barbora and Hajkova, Petra and Bryja, Josef and Zima, Jan Jr and Hajkova, Andrea and Zima, Jan},
article_number = {1},
keywords = {noninvasive genetic sampling; amplification success rate; ungulates},
language = {eng},
issn = {0139-7893},
journal = {Folia Zoologica},
title = {Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois},
url = {https://doi.org/10.25225/fozo.v60.i1.a11.2011},
volume = {60},
year = {2011}
}
TY - JOUR
ID - 1664017
AU - Zemanová, Barbora - Hajkova, Petra - Bryja, Josef - Zima, Jan Jr - Hajkova, Andrea - Zima, Jan
PY - 2011
TI - Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois
JF - Folia Zoologica
VL - 60
IS - 1
SP - 70-80
EP - 70-80
PB - INST VERTEBRATE BIOLOGY AS CR
SN - 01397893
KW - noninvasive genetic sampling
KW - amplification success rate
KW - ungulates
UR - https://doi.org/10.25225/fozo.v60.i1.a11.2011
L2 - https://doi.org/10.25225/fozo.v60.i1.a11.2011
N2 - The only autochthonous population of Tatra chamois (Rupicapra rupicapra tatrica) occurs in the Tatra Mountains (northern Slovakia and southern Poland). Another population has been introduced to the Low Tatra Mts., while Alpine chamois (R. r. rupicapra) has been introduced to the neighbouring mountain ranges, Velka Fatra and Slovensky raj. All these populations have undergone intensive bottlenecks. Any resulting low genetic variability would mean that only few genetic markers could be used for population genetic studies due to prevailing monomorphism. We tested 65 markers previously used in chamois or other Caprinae species, from which 20 most suitable loci for noninvasive genetic study of the Tatra chamois were selected. These polymorphic loci were used for optimisation of three multiplex sets and revealed a mean number of alleles of 2.1 and mean expected heterozygosity of 0.331 for the Tatra population. Low genetic diversity was also observed in the Low Tatra population while slightly higher values were obtained for Alpine chamois population in Slovensky raj. We subsequently assessed the amplification success rate for noninvasively obtained samples (faeces), which ranged from 85.1% to 92.7% for particular loci. The developed polymorphic microsatellite sets provide a unique tool for population genetic study of the endangered Tatra chamois, even when using noninvasive sampling, and is also suitable for Alpine chamois.
ER -
ZEMANOVÁ, Barbora, Petra HAJKOVA, Josef BRYJA, Jan Jr ZIMA, Andrea HAJKOVA and Jan ZIMA. Development of multiplex microsatellite sets for noninvasive population genetic study of the endangered Tatra chamois. \textit{Folia Zoologica}. INST VERTEBRATE BIOLOGY AS CR, 2011, vol.~60, No~1, p.~70-80. ISSN~0139-7893.
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