Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry

KŘEMENOVÁ, Jana, Ondřej BALVÍN, Oliver OTTI, Michal PAVONIČ, Klaus REINHARDT, Zdeněk ŠIMEK and Tomáš BARTONIČKA. Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry. Scientific reports. London: Nature Publishing Group, 2020, vol. 10, No 1, p. 1-14. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-66919-5.

Basic information

• Original name: Identification and age-dependence of pteridines in bed bugs (Cimex lectularius) and bat bugs (C. pipistrelli) using liquid chromatography-tandem mass spectrometry
• Authors: KŘEMENOVÁ, Jana (203 Czech Republic, guarantor, belonging to the institution), Ondřej BALVÍN, Oliver OTTI, Michal PAVONIČ (203 Czech Republic, belonging to the institution), Klaus REINHARDT, Zdeněk ŠIMEK (203 Czech Republic, belonging to the institution) and Tomáš BARTONIČKA (203 Czech Republic, belonging to the institution).
• Edition: Scientific reports, London, Nature Publishing Group, 2020, 2045-2322.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10613 Zoology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 4.379
• RIV identification code: RIV/00216224:14310/20:00114207
• Organization unit: Faculty of Science
• Doi: http://dx.doi.org/10.1038/s41598-020-66919-5
• UT WoS: 000543993000023
• Keywords in English: fluorescent pigments; haematophagy; method optimization; insect; age-grading; LC-MS/MS
• Tags: rivok
• Tags: International impact, Reviewed

Abstract

Determining the age of free-living insects, particularly of blood-sucking species, is important for human health because such knowledge critically influences the estimates of biting frequency and vectoring ability. Genetic age determination is currently not available. Pteridines gradually accumulate in the eyes of insects and their concentrations is the prevailing method. Despite of their stability, published extractions differ considerably, including for standards, for mixtures of pteridines and even for light conditions. This methodological inconsistency among studies is likely to influence age estimates severely and to hamper their comparability. Therefore we reviewed methodological steps across 106 studies to identify methodological denominators and results across studies. Second, we experimentally test how different pteridines vary in their age calibration curves in, common bed (Cimex lectularius) and bat bugs (C. pipistrelli). Here we show that the accumulation of particular pteridines varied between a) different populations and b) rearing temperatures but not c) with the impact of light conditions during extraction or d) the type of blood consumed by the bugs. To optimize the extraction of pteridines and measuring concentrations, we recommend the simultaneous measurement of more than one standard and subsequently to select those that show consistent changes over time to differentiate among age cohorts.

Links

• GC18-08468J, research and development project: Name: Role adaptace a fenotypové plasticity spermií v ekologické speciaci

Investor: Czech Science Foundation

• MUNI/A/1436/2018, interní kód MU: Name: EKologické a EVOluční Principy v populacích obratlovců a jejich parazitů (Acronym: EKEVOP)

Investor: Masaryk University, Category A