Detailed Information on Publication Record
2020
Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor
HUANG, Shengyun, Andrea CELÁ, Erwin ADAMS, Zdeněk GLATZ, Ann VAN SCHEPDAEL et. al.Basic information
Original name
Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor
Name in Czech
BStanoveni AO pomocí CE
Authors
HUANG, Shengyun (156 China), Andrea CELÁ (203 Czech Republic, belonging to the institution), Erwin ADAMS (56 Belgium), Zdeněk GLATZ (203 Czech Republic, belonging to the institution) and Ann VAN SCHEPDAEL (56 Belgium, guarantor)
Edition
Journal of Separation Science, Weinheim, Wiley, 2020, 1615-9306
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10406 Analytical chemistry
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.645
RIV identification code
RIV/00216224:14310/20:00114212
Organization unit
Faculty of Science
UT WoS
000550474000001
Keywords (in Czech)
Enzymy; Stanovení aktivity; kapilární electrophores; IMER
Keywords in English
Enzymes; Assay; Capillary electrophoresis; IMER
Tags
Tags
International impact, Reviewed
Změněno: 21/1/2021 13:45, Mgr. Marie Šípková, DiS.
V originále
CE is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of enzyme kinetics during drug development. Homogenous or heterogenous approaches can be followed for the enzymatic incubation. In this study, an immobilization procedure of aldehyde oxidase on magnetic particles was developed considering their integration with CE. A number of magnetic nano/microparticle types were tested for this purpose, showing that aldehyde oxidase was most active when immobilized on bare silica magnetic nanoparticles.
In Czech
Byla vypracována metoda pro imobilizaci AO na magnetická částice za účele konstrukce kombinace CE-IMER.
Links
| GA19-08358S, research and development project |
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| MUNI/A/1265/2015, interní kód MU |
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