| HUANG, Shengyun, Andrea CELÁ, Erwin ADAMS, Zdeněk GLATZ and Ann VAN SCHEPDAEL. Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor. Journal of Separation Science. Weinheim: Wiley, 2020, vol. 43, No 17, p. 3565-3572. ISSN 1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202000412. |
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@article{1668581,
author = {Huang, Shengyun and Celá, Andrea and Adams, Erwin and Glatz, Zdeněk and Van Schepdael, Ann},
article_location = {Weinheim},
article_number = {17},
doi = {http://dx.doi.org/10.1002/jssc.202000412},
keywords = {Enzymes; Assay; Capillary electrophoresis; IMER},
language = {eng},
issn = {1615-9306},
journal = {Journal of Separation Science},
title = {Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor},
url = {https://doi.org/10.1002/jssc.202000412},
volume = {43},
year = {2020}
}
TY - JOUR
ID - 1668581
AU - Huang, Shengyun - Celá, Andrea - Adams, Erwin - Glatz, Zdeněk - Van Schepdael, Ann
PY - 2020
TI - Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor
JF - Journal of Separation Science
VL - 43
IS - 17
SP - 3565-3572
EP - 3565-3572
PB - Wiley
SN - 16159306
KW - Enzymes
KW - Assay
KW - Capillary electrophoresis
KW - IMER
UR - https://doi.org/10.1002/jssc.202000412
L2 - https://doi.org/10.1002/jssc.202000412
N2 - CE is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of enzyme kinetics during drug development. Homogenous or heterogenous approaches can be followed for the enzymatic incubation. In this study, an immobilization procedure of aldehyde oxidase on magnetic particles was developed considering their integration with CE. A number of magnetic nano/microparticle types were tested for this purpose, showing that aldehyde oxidase was most active when immobilized on bare silica magnetic nanoparticles.
ER -
HUANG, Shengyun, Andrea CELÁ, Erwin ADAMS, Zdeněk GLATZ and Ann VAN SCHEPDAEL. Aldehyde Oxidase Assay by CE: from off-line, on-line up to immobilized enzyme reactor. \textit{Journal of Separation Science}. Weinheim: Wiley, 2020, vol.~43, No~17, p.~3565-3572. ISSN~1615-9306. Available from: https://dx.doi.org/10.1002/jssc.202000412.
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