N-Glycan profiling of lung adenocarcinoma in patients at
different stages of disease

J 2020

N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease

LATTOVÁ, Erika, Jana SKŘIČKOVÁ, Jitka HAUSNEROVÁ, Lukáš FROLA, Leoš KŘEN et. al.

Basic information

Original name

N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease

Authors

LATTOVÁ, Erika (703 Slovakia, guarantor, belonging to the institution), Jana SKŘIČKOVÁ (203 Czech Republic, belonging to the institution), Jitka HAUSNEROVÁ (203 Czech Republic, belonging to the institution), Lukáš FROLA (203 Czech Republic, belonging to the institution), Leoš KŘEN (203 Czech Republic, belonging to the institution), Ivana IHNATOVÁ (703 Slovakia, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), J. BRYANT and M. POPOVIC

Edition

MODERN PATHOLOGY, NEW YORK, NATURE PUBLISHING GROUP, 2020, 0893-3952

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30109 Pathology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.842

RIV identification code

RIV/00216224:14740/20:00115918

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/s41379-019-0441-3

UT WoS

000508157700002

Keywords in English

GLYCOSYLATION; FUCOSYLATION; GLYCOME; PROTEIN; TISSUE

Abstract

V originále

Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age. Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood. In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC. Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease. The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal(2)GlcNAc(2)Man(3)GlcNAc(2)Fuc were general features of tumors. In addition, 42 new N-glycan compositions were detected in cancerous tissues. The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units. Both of these monosaccharide types were linked preferably on the 6-antenna. Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy. In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies. Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.

Links

EF17_050/0008496, research and development project

Name: MSCAfellow@MUNI

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90043, large research infrastructures

Name: CIISB

Files attached

s41379-019-0441-3.pdf

Find similar documents

Citovat

LATTOVÁ, Erika, Jana SKŘIČKOVÁ, Jitka HAUSNEROVÁ, Lukáš FROLA, Leoš KŘEN, Ivana IHNATOVÁ, Zbyněk ZDRÁHAL, J. BRYANT and M. POPOVIC. N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease. MODERN PATHOLOGY. NEW YORK: NATURE PUBLISHING GROUP, 2020, vol. 33, No 6, p. 1146-1156. ISSN 0893-3952. Available from: https://dx.doi.org/10.1038/s41379-019-0441-3.

@article{1669311,
   author = {Lattová, Erika and Skřičková, Jana and Hausnerová, Jitka and Frola, Lukáš and Křen, Leoš and Ihnatová, Ivana and Zdráhal, Zbyněk and Bryant, J. and Popovic, M.},
   article_location = {NEW YORK},
   article_number = {6},
   doi = {http://dx.doi.org/10.1038/s41379-019-0441-3},
   keywords = {GLYCOSYLATION; FUCOSYLATION; GLYCOME; PROTEIN; TISSUE},
   language = {eng},
   issn = {0893-3952},
   journal = {MODERN PATHOLOGY},
   title = {N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease},
   url = {https://www.nature.com/articles/s41379-019-0441-3.pdf},
   volume = {33},
   year = {2020}
}

TY  - JOUR
ID  - 1669311
AU  - Lattová, Erika - Skřičková, Jana - Hausnerová, Jitka - Frola, Lukáš - Křen, Leoš - Ihnatová, Ivana - Zdráhal, Zbyněk - Bryant, J. - Popovic, M.
PY  - 2020
TI  - N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease
JF  - MODERN PATHOLOGY
VL  - 33
IS  - 6
SP  - 1146-1156
EP  - 1146-1156
PB  - NATURE PUBLISHING GROUP
SN  - 08933952
KW  - GLYCOSYLATION
KW  - FUCOSYLATION
KW  - GLYCOME
KW  - PROTEIN
KW  - TISSUE
UR  - https://www.nature.com/articles/s41379-019-0441-3.pdf
L2  - https://www.nature.com/articles/s41379-019-0441-3.pdf
N2  - Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age. Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood. In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC. Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease. The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal(2)GlcNAc(2)Man(3)GlcNAc(2)Fuc were general features of tumors. In addition, 42 new N-glycan compositions were detected in cancerous tissues. The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units. Both of these monosaccharide types were linked preferably on the 6-antenna. Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy. In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies. Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.
ER  -

LATTOVÁ, Erika, Jana SKŘIČKOVÁ, Jitka HAUSNEROVÁ, Lukáš FROLA, Leoš KŘEN, Ivana IHNATOVÁ, Zbyněk ZDRÁHAL, J. BRYANT and M. POPOVIC. N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease. \textit{MODERN PATHOLOGY}. NEW YORK: NATURE PUBLISHING GROUP, 2020, vol.~33, No~6, p.~1146-1156. ISSN~0893-3952. Available from: https://dx.doi.org/10.1038/s41379-019-0441-3.

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