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@article{1669311,
author = {Lattová, Erika and Skřičková, Jana and Hausnerová, Jitka and Frola, Lukáš and Křen, Leoš and Ihnatová, Ivana and Zdráhal, Zbyněk and Bryant, J. and Popovic, M.},
article_location = {NEW YORK},
article_number = {6},
doi = {http://dx.doi.org/10.1038/s41379-019-0441-3},
keywords = {GLYCOSYLATION; FUCOSYLATION; GLYCOME; PROTEIN; TISSUE},
language = {eng},
issn = {0893-3952},
journal = {MODERN PATHOLOGY},
title = {N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease},
url = {https://www.nature.com/articles/s41379-019-0441-3.pdf},
volume = {33},
year = {2020}
}
TY - JOUR
ID - 1669311
AU - Lattová, Erika - Skřičková, Jana - Hausnerová, Jitka - Frola, Lukáš - Křen, Leoš - Ihnatová, Ivana - Zdráhal, Zbyněk - Bryant, J. - Popovic, M.
PY - 2020
TI - N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease
JF - MODERN PATHOLOGY
VL - 33
IS - 6
SP - 1146-1156
EP - 1146-1156
PB - NATURE PUBLISHING GROUP
SN - 08933952
KW - GLYCOSYLATION
KW - FUCOSYLATION
KW - GLYCOME
KW - PROTEIN
KW - TISSUE
UR - https://www.nature.com/articles/s41379-019-0441-3.pdf
L2 - https://www.nature.com/articles/s41379-019-0441-3.pdf
N2 - Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age. Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood. In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC. Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease. The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal(2)GlcNAc(2)Man(3)GlcNAc(2)Fuc were general features of tumors. In addition, 42 new N-glycan compositions were detected in cancerous tissues. The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units. Both of these monosaccharide types were linked preferably on the 6-antenna. Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy. In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies. Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.
ER -
LATTOVÁ, Erika, Jana SKŘIČKOVÁ, Jitka HAUSNEROVÁ, Lukáš FROLA, Leoš KŘEN, Ivana IHNATOVÁ, Zbyněk ZDRÁHAL, J. BRYANT and M. POPOVIC. N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease. \textit{MODERN PATHOLOGY}. NEW YORK: NATURE PUBLISHING GROUP, 2020, vol.~33, No~6, p.~1146-1156. ISSN~0893-3952. Available from: https://dx.doi.org/10.1038/s41379-019-0441-3.
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