Nitrogen nutrition modulates oxidative stress and metabolite
production in Hypericum perforatum

J 2020

Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum

KOVÁČIK, Jozef, Slawomir DRESLER, Viera PETERKOVÁ and Petr BABULA

Basic information

Original name

Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum

Authors

KOVÁČIK, Jozef (703 Slovakia, guarantor), Slawomir DRESLER (616 Poland), Viera PETERKOVÁ (703 Slovakia) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

Protoplasma, Wien, Springer-Verlag, 2020, 0033-183X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Austria

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.356

RIV identification code

RIV/00216224:14110/20:00115971

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s00709-019-01448-1

UT WoS

000519379600009

Keywords in English

Antioxidants; Fluorescence microscopy; Mineral nutrition; Phenols; Secondary metabolites

Abstract

V originále

Impact of various nitrate concentrations (14.12 mM, 3.53 mM, no nitrate) or ammonium presence (14.12 mM) on physiological and metabolic changes in Hypericum perforatum after 14 days of cultivation was monitored. Nitrate deficiency suppressed growth of shoots but stimulated root growth while ammonium suppressed root growth: concomitant changes of ascorbic acid and glutathione supported these growth changes, e.g., unaltered level in roots under nitrate deficiency but depleted in ammonium treatment. Soluble proteins and water content were more suppressed by nitrate deficiency but total ROS, nitric oxide formation, and antioxidative enzyme activities (APX and SOD) indicate higher sensitivity of plants to ammonium. Though both extreme treatments (NO3- deficiency or ammonium) stimulated accumulation of total soluble phenols and affected PAL activity (in comparison with full or 1/4x nitrate dose), major phenols (chlorogenic acid and three flavonoids) were elevated mainly by NO3- deficiency. At the level of specific metabolites, NO3- deficiency had stimulatory impact on pseudohypericin (but not hypericin) content while hyperforin decreased. Expression of earlier putative gene of hypericin biosynthesis (hyp-1) showed rather partial correlation with pseudohypericin amount. Data indicate that depletion of NO3- is useful to obtain Hypericum plants with higher amount of health-positive secondary metabolites.

Citovat

KOVÁČIK, Jozef, Slawomir DRESLER, Viera PETERKOVÁ and Petr BABULA. Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum. Protoplasma. Wien: Springer-Verlag, 2020, vol. 257, No 2, p. 439-447. ISSN 0033-183X. Available from: https://dx.doi.org/10.1007/s00709-019-01448-1.

@article{1670235,
   author = {Kováčik, Jozef and Dresler, Slawomir and Peterková, Viera and Babula, Petr},
   article_location = {Wien},
   article_number = {2},
   doi = {http://dx.doi.org/10.1007/s00709-019-01448-1},
   keywords = {Antioxidants; Fluorescence microscopy; Mineral nutrition; Phenols; Secondary metabolites},
   language = {eng},
   issn = {0033-183X},
   journal = {Protoplasma},
   title = {Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum},
   url = {https://link.springer.com/article/10.1007/s00709-019-01448-1},
   volume = {257},
   year = {2020}
}

TY  - JOUR
ID  - 1670235
AU  - Kováčik, Jozef - Dresler, Slawomir - Peterková, Viera - Babula, Petr
PY  - 2020
TI  - Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum
JF  - Protoplasma
VL  - 257
IS  - 2
SP  - 439-447
EP  - 439-447
PB  - Springer-Verlag
SN  - 0033183X
KW  - Antioxidants
KW  - Fluorescence microscopy
KW  - Mineral nutrition
KW  - Phenols
KW  - Secondary metabolites
UR  - https://link.springer.com/article/10.1007/s00709-019-01448-1
L2  - https://link.springer.com/article/10.1007/s00709-019-01448-1
N2  - Impact of various nitrate concentrations (14.12 mM, 3.53 mM, no nitrate) or ammonium presence (14.12 mM) on physiological and metabolic changes in Hypericum perforatum after 14 days of cultivation was monitored. Nitrate deficiency suppressed growth of shoots but stimulated root growth while ammonium suppressed root growth: concomitant changes of ascorbic acid and glutathione supported these growth changes, e.g., unaltered level in roots under nitrate deficiency but depleted in ammonium treatment. Soluble proteins and water content were more suppressed by nitrate deficiency but total ROS, nitric oxide formation, and antioxidative enzyme activities (APX and SOD) indicate higher sensitivity of plants to ammonium. Though both extreme treatments (NO3- deficiency or ammonium) stimulated accumulation of total soluble phenols and affected PAL activity (in comparison with full or 1/4x nitrate dose), major phenols (chlorogenic acid and three flavonoids) were elevated mainly by NO3- deficiency. At the level of specific metabolites, NO3- deficiency had stimulatory impact on pseudohypericin (but not hypericin) content while hyperforin decreased. Expression of earlier putative gene of hypericin biosynthesis (hyp-1) showed rather partial correlation with pseudohypericin amount. Data indicate that depletion of NO3- is useful to obtain Hypericum plants with higher amount of health-positive secondary metabolites.
ER  -

KOVÁČIK, Jozef, Slawomir DRESLER, Viera PETERKOVÁ and Petr BABULA. Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum. \textit{Protoplasma}. Wien: Springer-Verlag, 2020, vol.~257, No~2, p.~439-447. ISSN~0033-183X. Available from: https://dx.doi.org/10.1007/s00709-019-01448-1.

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