| SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA a Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Analytical chemistry. WASHINGTON: AMER CHEMICAL SOC, 2020, roč. 92, č. 11, s. 7588-7595. ISSN 0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.0c00165. |
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@article{1670470,
author = {Salplachta, Jiri and Horka, Marie and Karasek, Pavel and Růžička, Filip and Roth, Michal},
article_location = {WASHINGTON},
article_number = {11},
doi = {http://dx.doi.org/10.1021/acs.analchem.0c00165},
keywords = {MALDI-TOF MS; SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS; DIFFERENT INTERNAL DIAMETERS; INVASIVE FUNGAL-INFECTIONS; DESORPTION IONIZATION-TIME; METHICILLIN-RESISTANT; DIAGNOSTIC METHODS; NEUTRAL ANALYTES; 2 SEGMENTS; MICROORGANISMS},
language = {eng},
issn = {0003-2700},
journal = {Analytical chemistry},
title = {Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry},
url = {https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#},
volume = {92},
year = {2020}
}
TY - JOUR
ID - 1670470
AU - Salplachta, Jiri - Horka, Marie - Karasek, Pavel - Růžička, Filip - Roth, Michal
PY - 2020
TI - Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
JF - Analytical chemistry
VL - 92
IS - 11
SP - 7588-7595
EP - 7588-7595
PB - AMER CHEMICAL SOC
SN - 00032700
KW - MALDI-TOF MS
KW - SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS
KW - DIFFERENT INTERNAL DIAMETERS
KW - INVASIVE FUNGAL-INFECTIONS
KW - DESORPTION IONIZATION-TIME
KW - METHICILLIN-RESISTANT
KW - DIAGNOSTIC METHODS
KW - NEUTRAL ANALYTES
KW - 2 SEGMENTS
KW - MICROORGANISMS
UR - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
L2 - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
N2 - Diagnosis of fungal infection in lung parenchyma is relatively difficult. Bronchoscopy with bronchoalveolar lavage is very useful in its diagnosing. Therefore, a method for rapid online concentration and analysis of Aspergillus conidia in bronchoalveolar lavage fluid using the combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) with subsequent off-line identification of the separated conidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is described in this study. In the proposed procedure, conidia were first dynamically adhered onto the roughened part of the inner surface of a fused silica capillary prepared by etching with supercritical water. Then the adhered conidia were desorbed, concentrated, and separated using a combination of tITP and MEKC. Finally, the fractions containing the separated conidia were collected from the capillary and analyzed by MALDI-TOF MS. The adhesion efficiency under the optimized experimental conditions was about 80%. This rapid diagnosis will contribute to timely initiation of therapy and increase the patient's chances of survival.
ER -
SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA a Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. \textit{Analytical chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2020, roč.~92, č.~11, s.~7588-7595. ISSN~0003-2700. Dostupné z: https://dx.doi.org/10.1021/acs.analchem.0c00165.
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