Identification of Aspergillus Conidia in Bronchoalveolar Lavage
Using Offline Combination of Capillary Electrophoresis in
Supercritical Water-Treated Fused Silica Capillary and
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass
Spectrometry

J 2020

Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA, Michal ROTH et. al.

Basic information

Original name

Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Authors

SALPLACHTA, Jiri (203 Czech Republic, guarantor), Marie HORKA (203 Czech Republic), Pavel KARASEK (203 Czech Republic), Filip RŮŽIČKA (203 Czech Republic, belonging to the institution) and Michal ROTH (203 Czech Republic)

Edition

Analytical chemistry, WASHINGTON, AMER CHEMICAL SOC, 2020, 0003-2700

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.986

RIV identification code

RIV/00216224:14110/20:00114808

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1021/acs.analchem.0c00165

UT WoS

000538417400032

Keywords in English

MALDI-TOF MS; SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS; DIFFERENT INTERNAL DIAMETERS; INVASIVE FUNGAL-INFECTIONS; DESORPTION IONIZATION-TIME; METHICILLIN-RESISTANT; DIAGNOSTIC METHODS; NEUTRAL ANALYTES; 2 SEGMENTS; MICROORGANISMS

Abstract

V originále

Diagnosis of fungal infection in lung parenchyma is relatively difficult. Bronchoscopy with bronchoalveolar lavage is very useful in its diagnosing. Therefore, a method for rapid online concentration and analysis of Aspergillus conidia in bronchoalveolar lavage fluid using the combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) with subsequent off-line identification of the separated conidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is described in this study. In the proposed procedure, conidia were first dynamically adhered onto the roughened part of the inner surface of a fused silica capillary prepared by etching with supercritical water. Then the adhered conidia were desorbed, concentrated, and separated using a combination of tITP and MEKC. Finally, the fractions containing the separated conidia were collected from the capillary and analyzed by MALDI-TOF MS. The adhesion efficiency under the optimized experimental conditions was about 80%. This rapid diagnosis will contribute to timely initiation of therapy and increase the patient's chances of survival.

Links

NV16-29916A, research and development project

Name: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

VI20172020069, research and development project

Name: Vývoj moderních instrumentálních metod pro rychlou detekci a identifikaci vybraných B-agens a toxinů (Acronym: VMIMRDIBT)

Investor: Ministry of the Interior of the CR

Citovat

SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA and Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Analytical chemistry. WASHINGTON: AMER CHEMICAL SOC, 2020, vol. 92, No 11, p. 7588-7595. ISSN 0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.0c00165.

@article{1670470,
   author = {Salplachta, Jiri and Horka, Marie and Karasek, Pavel and Růžička, Filip and Roth, Michal},
   article_location = {WASHINGTON},
   article_number = {11},
   doi = {http://dx.doi.org/10.1021/acs.analchem.0c00165},
   keywords = {MALDI-TOF MS; SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS; DIFFERENT INTERNAL DIAMETERS; INVASIVE FUNGAL-INFECTIONS; DESORPTION IONIZATION-TIME; METHICILLIN-RESISTANT; DIAGNOSTIC METHODS; NEUTRAL ANALYTES; 2 SEGMENTS; MICROORGANISMS},
   language = {eng},
   issn = {0003-2700},
   journal = {Analytical chemistry},
   title = {Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry},
   url = {https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#},
   volume = {92},
   year = {2020}
}

TY  - JOUR
ID  - 1670470
AU  - Salplachta, Jiri - Horka, Marie - Karasek, Pavel - Růžička, Filip - Roth, Michal
PY  - 2020
TI  - Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry
JF  - Analytical chemistry
VL  - 92
IS  - 11
SP  - 7588-7595
EP  - 7588-7595
PB  - AMER CHEMICAL SOC
SN  - 00032700
KW  - MALDI-TOF MS
KW  - SUSCEPTIBLE STAPHYLOCOCCUS-AUREUS
KW  - DIFFERENT INTERNAL DIAMETERS
KW  - INVASIVE FUNGAL-INFECTIONS
KW  - DESORPTION IONIZATION-TIME
KW  - METHICILLIN-RESISTANT
KW  - DIAGNOSTIC METHODS
KW  - NEUTRAL ANALYTES
KW  - 2 SEGMENTS
KW  - MICROORGANISMS
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
L2  - https://pubs.acs.org/doi/10.1021/acs.analchem.0c00165#
N2  - Diagnosis of fungal infection in lung parenchyma is relatively difficult. Bronchoscopy with bronchoalveolar lavage is very useful in its diagnosing. Therefore, a method for rapid online concentration and analysis of Aspergillus conidia in bronchoalveolar lavage fluid using the combination of transient isotachophoresis (tITP) and micellar electrokinetic chromatography (MEKC) with subsequent off-line identification of the separated conidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is described in this study. In the proposed procedure, conidia were first dynamically adhered onto the roughened part of the inner surface of a fused silica capillary prepared by etching with supercritical water. Then the adhered conidia were desorbed, concentrated, and separated using a combination of tITP and MEKC. Finally, the fractions containing the separated conidia were collected from the capillary and analyzed by MALDI-TOF MS. The adhesion efficiency under the optimized experimental conditions was about 80%. This rapid diagnosis will contribute to timely initiation of therapy and increase the patient's chances of survival.
ER  -

SALPLACHTA, Jiri, Marie HORKA, Pavel KARASEK, Filip RŮŽIČKA and Michal ROTH. Identification of Aspergillus Conidia in Bronchoalveolar Lavage Using Offline Combination of Capillary Electrophoresis in Supercritical Water-Treated Fused Silica Capillary and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. \textit{Analytical chemistry}. WASHINGTON: AMER CHEMICAL SOC, 2020, vol.~92, No~11, p.~7588-7595. ISSN~0003-2700. Available from: https://dx.doi.org/10.1021/acs.analchem.0c00165.

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