The Beta Subunit of Nascent Polypeptide Associated Complex
Plays A Role in Flowers and Siliques Development of Arabidopsis
thaliana

J 2020

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK et. al.

Basic information

Original name

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

Authors

FÍLA, Jan (203 Czech Republic), Božena KLODOVÁ (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution) and David HONYS

Edition

International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 5.923

RIV identification code

RIV/00216224:14740/20:00114234

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms21062065

UT WoS

000529890200161

Keywords in English

Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex

Abstract

V originále

The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, alpha and beta, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NAC alpha subunit, and two genes encoding NAC beta. Double homozygous mutant in both genes coding for NAC beta was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NAC beta genes were characterized in more detail-the phenotype of the double homozygous mutant was complemented by a functional NAC beta copy. Then, both NAC beta genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nac beta mutation, the flower buds' transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NAC beta subunits in stress responses, male gametophyte development, and photosynthesis.

Links

EF16_019/0000738, research and development project

Name: Centrum experimentální biologie rostlin

GA18-02448S, research and development project

Name: Úloha translačních iniciačních faktorů při skladování a aktivaci transkriptů v samčí zárodečné linii krytosemenných rostlin

Investor: Czech Science Foundation

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL and David HONYS. The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana. International Journal of Molecular Sciences. Basel: MDPI, 2020, vol. 21, No 6, p. 1-30. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21062065.

@article{1670471,
   author = {Fíla, Jan and Klodová, Božena and Potěšil, David and Juříček, Miloslav and Šesták, Petr and Zdráhal, Zbyněk and Honys, David},
   article_location = {Basel},
   article_number = {6},
   doi = {http://dx.doi.org/10.3390/ijms21062065},
   keywords = {Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana},
   url = {https://www.mdpi.com/1422-0067/21/6/2065/htm},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1670471
AU  - Fíla, Jan - Klodová, Božena - Potěšil, David - Juříček, Miloslav - Šesták, Petr - Zdráhal, Zbyněk - Honys, David
PY  - 2020
TI  - The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 6
SP  - 1-30
EP  - 1-30
PB  - MDPI
SN  - 14220067
KW  - Arabidopsis thaliana
KW  - chaperone
KW  - flower bud proteome
KW  - flower bud transcriptome
KW  - male gametophyte
KW  - nascent polypeptide-associated complex
UR  - https://www.mdpi.com/1422-0067/21/6/2065/htm
L2  - https://www.mdpi.com/1422-0067/21/6/2065/htm
N2  - The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, alpha and beta, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NAC alpha subunit, and two genes encoding NAC beta. Double homozygous mutant in both genes coding for NAC beta was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NAC beta genes were characterized in more detail-the phenotype of the double homozygous mutant was complemented by a functional NAC beta copy. Then, both NAC beta genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nac beta mutation, the flower buds' transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NAC beta subunits in stress responses, male gametophyte development, and photosynthesis.
ER  -

FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL and David HONYS. The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, vol.~21, No~6, p.~1-30. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21062065.

Detailed Information on Publication Record