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@article{1670471,
author = {Fíla, Jan and Klodová, Božena and Potěšil, David and Juříček, Miloslav and Šesták, Petr and Zdráhal, Zbyněk and Honys, David},
article_location = {Basel},
article_number = {6},
doi = {http://dx.doi.org/10.3390/ijms21062065},
keywords = {Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana},
url = {https://www.mdpi.com/1422-0067/21/6/2065/htm},
volume = {21},
year = {2020}
}
TY - JOUR
ID - 1670471
AU - Fíla, Jan - Klodová, Božena - Potěšil, David - Juříček, Miloslav - Šesták, Petr - Zdráhal, Zbyněk - Honys, David
PY - 2020
TI - The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana
JF - International Journal of Molecular Sciences
VL - 21
IS - 6
SP - 1-30
EP - 1-30
PB - MDPI
SN - 14220067
KW - Arabidopsis thaliana
KW - chaperone
KW - flower bud proteome
KW - flower bud transcriptome
KW - male gametophyte
KW - nascent polypeptide-associated complex
UR - https://www.mdpi.com/1422-0067/21/6/2065/htm
L2 - https://www.mdpi.com/1422-0067/21/6/2065/htm
N2 - The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, alpha and beta, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NAC alpha subunit, and two genes encoding NAC beta. Double homozygous mutant in both genes coding for NAC beta was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NAC beta genes were characterized in more detail-the phenotype of the double homozygous mutant was complemented by a functional NAC beta copy. Then, both NAC beta genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nac beta mutation, the flower buds' transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NAC beta subunits in stress responses, male gametophyte development, and photosynthesis.
ER -
FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL and David HONYS. The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, vol.~21, No~6, p.~1-30. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21062065.
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