J 2020

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

FÍLA, Jan, Božena KLODOVÁ, David POTĚŠIL, Miloslav JUŘÍČEK, Petr ŠESTÁK et. al.

Basic information

Original name

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

Authors

FÍLA, Jan (203 Czech Republic), Božena KLODOVÁ (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Miloslav JUŘÍČEK, Petr ŠESTÁK, Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution) and David HONYS

Edition

International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 5.923

RIV identification code

RIV/00216224:14740/20:00114234

Organization unit

Central European Institute of Technology

DOI

UT WoS

000529890200161

Keywords in English

Arabidopsis thaliana; chaperone; flower bud proteome; flower bud transcriptome; male gametophyte; nascent polypeptide-associated complex

Tags

Změněno: 10/10/2024 14:02, Ing. Martina Blahová

Abstract

V originále

The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, alpha and beta, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NAC alpha subunit, and two genes encoding NAC beta. Double homozygous mutant in both genes coding for NAC beta was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NAC beta genes were characterized in more detail-the phenotype of the double homozygous mutant was complemented by a functional NAC beta copy. Then, both NAC beta genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nac beta mutation, the flower buds' transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NAC beta subunits in stress responses, male gametophyte development, and photosynthesis.

Links

EF16_019/0000738, research and development project
Name: Centrum experimentální biologie rostlin
GA18-02448S, research and development project
Name: Úloha translačních iniciačních faktorů při skladování a aktivaci transkriptů v samčí zárodečné linii krytosemenných rostlin
Investor: Czech Science Foundation
LM2018127, research and development project
Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)
Investor: Ministry of Education, Youth and Sports of the CR
LM2018140, research and development project
Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)
Investor: Ministry of Education, Youth and Sports of the CR
90127, large research infrastructures
Name: CIISB II