J 2020

Matrix metalloproteinases gene variants and dental caries in Czech children

BOŘILOVÁ LINHARTOVÁ, Petra, Tereza DEISSOVÁ, Martina KUKLETOVÁ and Lydie IZAKOVIČOVÁ HOLLÁ

Basic information

Original name

Matrix metalloproteinases gene variants and dental caries in Czech children

Authors

BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, belonging to the institution), Tereza DEISSOVÁ (203 Czech Republic, belonging to the institution), Martina KUKLETOVÁ (203 Czech Republic, belonging to the institution) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

BMC Oral Health, LONDON, BMC, 2020, 1472-6831

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30208 Dentistry, oral surgery and medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 2.757

RIV identification code

RIV/00216224:14110/20:00116012

Organization unit

Faculty of Medicine

DOI

UT WoS

000535616700001

Keywords in English

Polymorphism; Association study; Caries; Oral disease; Genetic predisposition; ELSPAC

Tags

Tags

International impact, Reviewed

Abstract

V originále

Background Matrix metalloproteinases (MMPs) play an important role in tooth formation and the mineralization of dental tissue. The aim of the study was to analyse Czech children with primary/permanent dentition polymorphisms in those genes encoding MMP2, MMP3, MMP9, MMP13, MMP16, and MMP20, which had been previously associated with dental caries in other populations. Methods In total, 782 Czech children were included in this case-control study. DNA samples were taken from 474 subjects with dental caries (with decayed/missing/filled teeth, DMFT >= 1) and 155 caries free children (DMFT = 0) aged 13-15 years, as well as 101 preschool children with early childhood caries (ECC, dmft >= 1) and 52 caries free children (dmft = 0), were analyzed for nine MMPs single nucleotide polymorphisms (SNPs) using real time polymerase chain reaction TaqMan assays. Results There were no significant differences in the allele and/or genotype frequencies of all the studied MMPs SNPs among children with dental caries in primary/permanent dentition and the healthy controls (P > 0.05). In addition, similar allele or genotype frequencies of the studied MMPs SNPs were found in children with severe dental caries in their permanent teeth (children with DMFT >= 6) and the healthy controls (DMFT = 0, P > 0.05). Conclusions This study demonstrated the lack of association between the selected SNPs in candidate genes of MMPs and the susceptibility to or severity of dental caries in both primary and permanent dentitions in Czech children.

Links

EF16_013/0001761, research and development project
Name: RECETOX RI
MUNI/A/1428/2019, interní kód MU
Name: Onemocnění dutiny ústní – etiopatogeneze, diagnostika a léčba
Investor: Masaryk University, Category A
NV17-30439A, research and development project
Name: Moderní biotechnologické a behaviorální přístupy ve výzkumu zubního kazu a strategie jeho prevence
ROZV/23/LF1/2019, interní kód MU
Name: Studium genetické predispozice hostitele a jeho mikrobiomu ve vztahu k chorobám dutiny ústní na jícnu
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects