Detailed Information on Publication Record
2020
Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains
MIKALOVÁ, Lenka, Klára JANEČKOVÁ, Markéta NOVÁKOVÁ, Michal STROUHAL, Darina ČEJKOVÁ et. al.Basic information
Original name
Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains
Authors
MIKALOVÁ, Lenka (203 Czech Republic, belonging to the institution), Klára JANEČKOVÁ (203 Czech Republic, belonging to the institution), Markéta NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Darina ČEJKOVÁ (203 Czech Republic), K. N. HARPER (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)
Edition
Plos One, San Francisco, Public Library Science, 2020, 1932-6203
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10603 Genetics and heredity
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.240
RIV identification code
RIV/00216224:14110/20:00114235
Organization unit
Faculty of Medicine
UT WoS
000535938700042
Keywords in English
ANTIGENIC VARIATION; SYPHILIS; DIVERSITY; REGIONS; NICHOLS; YAWS; CELL
Tags
Tags
International impact, Reviewed
Změněno: 23/2/2021 14:39, Mgr. Tereza Miškechová
Abstract
V originále
Treponema pallidum subsp. endemicum (TEN) is the causative agent of endemic syphilis (bejel). Until now, only a single TEN strain, Bosnia A, has been completely sequenced. The only other laboratory TEN strain available, Iraq B, was isolated in Iraq in 1951 by researchers from the US Centers for Disease Control and Prevention. In this study, the complete genome of the Iraq B strain was amplified as overlapping PCR products and sequenced using the pooled segment genome sequencing method and Illumina sequencing. Total average genome sequencing coverage reached 3469x, with a total genome size of 1,137,653 bp. Compared to the genome sequence of Bosnia A, a set of 37 single nucleotide differences, 4 indels, 2 differences in the number of tandem repetitions, and 18 differences in the length of homopolymeric regions were found in the Iraq B genome. Moreover, the tprF and tprG genes that were previously found deleted in the genome of the TEN Bosnia A strain (spanning 2.3 kb in length) were present in a subpopulation of TEN Iraq B and Bosnia A microbes, and their sequence was highly similar to those found in T. p. subsp. pertenue strains, which cause the disease yaws. The genome sequence of TEN Iraq B revealed close genetic relatedness between both available bejel-causing laboratory strains (i.e., Iraq B and Bosnia A) and also genetic variability within the bejel treponemes comparable to that found within yaws- or syphilis-causing strains. In addition, genetic relatedness to TPE strains was demonstrated by the sequence of the tprF and tprG genes found in sub-populations of both TEN Iraq B and Bosnia A. The loss of the tprF and tprG genes in most TEN microbes suggest that TEN genomes have been evolving via the loss of genomic regions, a phenomenon previously found among the treponemes causing both syphilis and rabbit syphilis.
Links
GA17-25455S, research and development project |
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GC18-23521J, research and development project |
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GJ17-25589Y, research and development project |
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