J 2020

Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains

MIKALOVÁ, Lenka, Klára JANEČKOVÁ, Markéta NOVÁKOVÁ, Michal STROUHAL, Darina ČEJKOVÁ et. al.

Basic information

Original name

Whole genome sequence of the Treponema pallidum subsp. endemicum strain Iraq B: A subpopulation of bejel treponemes contains full-length tprF and tprG genes similar to those present in T. p. subsp. pertenue strains

Authors

MIKALOVÁ, Lenka (203 Czech Republic, belonging to the institution), Klára JANEČKOVÁ (203 Czech Republic, belonging to the institution), Markéta NOVÁKOVÁ (203 Czech Republic, belonging to the institution), Michal STROUHAL (203 Czech Republic, belonging to the institution), Darina ČEJKOVÁ (203 Czech Republic), K. N. HARPER (840 United States of America) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plos One, San Francisco, Public Library Science, 2020, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10603 Genetics and heredity

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.240

RIV identification code

RIV/00216224:14110/20:00114235

Organization unit

Faculty of Medicine

UT WoS

000535938700042

Keywords in English

ANTIGENIC VARIATION; SYPHILIS; DIVERSITY; REGIONS; NICHOLS; YAWS; CELL

Tags

Tags

International impact, Reviewed
Změněno: 23/2/2021 14:39, Mgr. Tereza Miškechová

Abstract

V originále

Treponema pallidum subsp. endemicum (TEN) is the causative agent of endemic syphilis (bejel). Until now, only a single TEN strain, Bosnia A, has been completely sequenced. The only other laboratory TEN strain available, Iraq B, was isolated in Iraq in 1951 by researchers from the US Centers for Disease Control and Prevention. In this study, the complete genome of the Iraq B strain was amplified as overlapping PCR products and sequenced using the pooled segment genome sequencing method and Illumina sequencing. Total average genome sequencing coverage reached 3469x, with a total genome size of 1,137,653 bp. Compared to the genome sequence of Bosnia A, a set of 37 single nucleotide differences, 4 indels, 2 differences in the number of tandem repetitions, and 18 differences in the length of homopolymeric regions were found in the Iraq B genome. Moreover, the tprF and tprG genes that were previously found deleted in the genome of the TEN Bosnia A strain (spanning 2.3 kb in length) were present in a subpopulation of TEN Iraq B and Bosnia A microbes, and their sequence was highly similar to those found in T. p. subsp. pertenue strains, which cause the disease yaws. The genome sequence of TEN Iraq B revealed close genetic relatedness between both available bejel-causing laboratory strains (i.e., Iraq B and Bosnia A) and also genetic variability within the bejel treponemes comparable to that found within yaws- or syphilis-causing strains. In addition, genetic relatedness to TPE strains was demonstrated by the sequence of the tprF and tprG genes found in sub-populations of both TEN Iraq B and Bosnia A. The loss of the tprF and tprG genes in most TEN microbes suggest that TEN genomes have been evolving via the loss of genomic regions, a phenomenon previously found among the treponemes causing both syphilis and rabbit syphilis.

Links

GA17-25455S, research and development project
Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk
Investor: Czech Science Foundation
GC18-23521J, research and development project
Name: Treponematózy u zástupců řádu zajícovci: genetická diverzita treponem a příbuznost s lidským patogenem T. pallidum
Investor: Czech Science Foundation
GJ17-25589Y, research and development project
Name: Kmeny způsobující syfilis: jsou geneticky odlišné kmeny odlišné i v průběhu experimentální infekce?
Investor: Czech Science Foundation