Long-term impact of cadmium in protonema cultures of
Physcomitrella patens

J 2020

Long-term impact of cadmium in protonema cultures of Physcomitrella patens

KOVACIK, Jozef, Slawomir DRESLER and Petr BABULA

Basic information

Original name

Long-term impact of cadmium in protonema cultures of Physcomitrella patens

Authors

KOVACIK, Jozef (703 Slovakia, guarantor), Slawomir DRESLER (616 Poland) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

Ecotoxicology and Environmental Safety, San Diego, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2020, 0147-6513

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Slovenia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.291

RIV identification code

RIV/00216224:14110/20:00116022

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.ecoenv.2020.110333

UT WoS

000521519900002

Keywords in English

Antioxidative enzymes; Fluorescence microscopy; Heavy metals; Oxidative stress

Abstract

V originále

Antioxidative responses of axenic protonema cultures of the moss Physcomitrella patens exposed to 10 mu M Cd over 40 d were studied. Cd treatment suppressed growth by ca. 75% with concomitant browning of some filaments and suppression of chlorophyll autofluorescence but had no impact on tissue water content. Despite this negative growth responses which could be related to enhanced ROS formation (as detected using fluorescence staining reagents for total ROS, hydroperoxides and lipid peroxidation), some metabolites revealed strong elevation by Cd which could contribute to attenuation of long-term Cd stress (elevation of ascorbic, malic and citric acids). Molar ratio of malate to Cd was 12.7 and citrate to Cd 2.5, thus potentially contributing to Cd chelation. Interestingly, GSH/GSSG pool and nitric oxide formation remained unaltered by Cd. Accumulation of Cd reached 82 mu g/g DW with bioaccumulation factor of 73. Data indicate that Cd induces elevation of potentially protective metabolites even after prolonged exposure though they do not prevent oxidative stress sufficiently.

Citovat

KOVACIK, Jozef, Slawomir DRESLER and Petr BABULA. Long-term impact of cadmium in protonema cultures of Physcomitrella patens. Ecotoxicology and Environmental Safety. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2020, vol. 193, April 2020, p. 1-6. ISSN 0147-6513. Available from: https://dx.doi.org/10.1016/j.ecoenv.2020.110333.

@article{1670604,
   author = {Kovacik, Jozef and Dresler, Slawomir and Babula, Petr},
   article_location = {San Diego},
   article_number = {April 2020},
   doi = {http://dx.doi.org/10.1016/j.ecoenv.2020.110333},
   keywords = {Antioxidative enzymes; Fluorescence microscopy; Heavy metals; Oxidative stress},
   language = {eng},
   issn = {0147-6513},
   journal = {Ecotoxicology and Environmental Safety},
   title = {Long-term impact of cadmium in protonema cultures of Physcomitrella patens},
   url = {https://www.sciencedirect.com/science/article/pii/S014765132030172X?via%3Dihub},
   volume = {193},
   year = {2020}
}

TY  - JOUR
ID  - 1670604
AU  - Kovacik, Jozef - Dresler, Slawomir - Babula, Petr
PY  - 2020
TI  - Long-term impact of cadmium in protonema cultures of Physcomitrella patens
JF  - Ecotoxicology and Environmental Safety
VL  - 193
IS  - April 2020
SP  - 1-6
EP  - 1-6
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 01476513
KW  - Antioxidative enzymes
KW  - Fluorescence microscopy
KW  - Heavy metals
KW  - Oxidative stress
UR  - https://www.sciencedirect.com/science/article/pii/S014765132030172X?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S014765132030172X?via%3Dihub
N2  - Antioxidative responses of axenic protonema cultures of the moss Physcomitrella patens exposed to 10 mu M Cd over 40 d were studied. Cd treatment suppressed growth by ca. 75% with concomitant browning of some filaments and suppression of chlorophyll autofluorescence but had no impact on tissue water content. Despite this negative growth responses which could be related to enhanced ROS formation (as detected using fluorescence staining reagents for total ROS, hydroperoxides and lipid peroxidation), some metabolites revealed strong elevation by Cd which could contribute to attenuation of long-term Cd stress (elevation of ascorbic, malic and citric acids). Molar ratio of malate to Cd was 12.7 and citrate to Cd 2.5, thus potentially contributing to Cd chelation. Interestingly, GSH/GSSG pool and nitric oxide formation remained unaltered by Cd. Accumulation of Cd reached 82 mu g/g DW with bioaccumulation factor of 73. Data indicate that Cd induces elevation of potentially protective metabolites even after prolonged exposure though they do not prevent oxidative stress sufficiently.
ER  -

KOVACIK, Jozef, Slawomir DRESLER and Petr BABULA. Long-term impact of cadmium in protonema cultures of Physcomitrella patens. \textit{Ecotoxicology and Environmental Safety}. San Diego: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2020, vol.~193, April 2020, p.~1-6. ISSN~0147-6513. Available from: https://dx.doi.org/10.1016/j.ecoenv.2020.110333.

Detailed Information on Publication Record