Selenium Incorporation to Amino Acids in Chlorella Cultures
Grown in Phototrophic and Heterotrophic Regimes

J 2020

Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes

MYLENKO, Mykola, Dai Long VU, Jan KUTA, Karolina RANGLOVA, David KUBÁČ et. al.

Basic information

Original name

Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes

Authors

MYLENKO, Mykola (203 Czech Republic), Dai Long VU (156 China), Jan KUTA (203 Czech Republic, guarantor, belonging to the institution), Karolina RANGLOVA (203 Czech Republic), David KUBÁČ (203 Czech Republic), Gergely LAKATOS (203 Czech Republic), Tomas GRIVALSKY (203 Czech Republic), Martin Pablo CAPORGNO (203 Czech Republic), Joao Artur Camara MANOEL (203 Czech Republic), Jiri KOPECKY (203 Czech Republic), Jiří MASOJÍDEK (203 Czech Republic) and Pavel HROUZEK (203 Czech Republic)

Edition

Journal of Agricultural and Food Chemistry, Washington, D.C. American Chemical Society, 2020, 0021-8561

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21101 Food and beverages

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.279

RIV identification code

RIV/00216224:14310/20:00116084

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1021/acs.jafc.9b06196

UT WoS

000514256200016

Keywords in English

Chlorella; microalgae; selenium; selenomethionine; selenocysteine; methylselenocysteine; phototrophic cultivation; heterotrophic cultivation

Abstract

V originále

Microalgae accumulate bioavailable selenium-containing amino acids (Se-AAs), and these are useful as a food supplement. While this accumulation has been studied in phototrophic algal cultures, little data exists for heterotrophic cultures. We have determined the Se-AAs content, selenium/sulfur (Se/S) substitution rates, and overall Se accumulation balance in photo-and heterotrophic Chlorella cultures. Laboratory trials revealed that heterotrophic cultures tolerate Se doses, similar to 8-fold higher compared to phototrophic cultures, resulting in a similar to 2-3-fold higher Se-AAs content. In large-scale experiments, both cultivation regimes provided comparable Se-AAs content. Outdoor phototrophic cultures accumulated up to 400 mu g g(-1) of total Se-AAs and exhibited a high level of Se/S substitution (5-10%) with 30-60% organic/total Se embedded in the biomass. A slightly higher content of Se-AAs and ratio of Se/S substitution was obtained for a heterotrophic culture in pilot-scale fermentors. The data presented here shows that heterotrophic Chlorella cultures provide an alternative for Se-enriched biomass production and provides information on Se-AAs content and speciation in different cultivation regimes.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MYLENKO, Mykola, Dai Long VU, Jan KUTA, Karolina RANGLOVA, David KUBÁČ, Gergely LAKATOS, Tomas GRIVALSKY, Martin Pablo CAPORGNO, Joao Artur Camara MANOEL, Jiri KOPECKY, Jiří MASOJÍDEK and Pavel HROUZEK. Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes. Journal of Agricultural and Food Chemistry. Washington, D.C.: American Chemical Society, 2020, vol. 68, No 6, p. 1654-1665. ISSN 0021-8561. Available from: https://dx.doi.org/10.1021/acs.jafc.9b06196.

@article{1672104,
   author = {Mylenko, Mykola and Vu, Dai Long and Kuta, Jan and Ranglova, Karolina and Kubáč, David and Lakatos, Gergely and Grivalsky, Tomas and Caporgno, Martin Pablo and Manoel, Joao Artur Camara and Kopecky, Jiri and Masojídek, Jiří and Hrouzek, Pavel},
   article_location = {Washington, D.C.},
   article_number = {6},
   doi = {http://dx.doi.org/10.1021/acs.jafc.9b06196},
   keywords = {Chlorella; microalgae; selenium; selenomethionine; selenocysteine; methylselenocysteine; phototrophic cultivation; heterotrophic cultivation},
   language = {eng},
   issn = {0021-8561},
   journal = {Journal of Agricultural and Food Chemistry},
   title = {Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes},
   url = {https://pubs.acs.org/doi/10.1021/acs.jafc.9b06196},
   volume = {68},
   year = {2020}
}

TY  - JOUR
ID  - 1672104
AU  - Mylenko, Mykola - Vu, Dai Long - Kuta, Jan - Ranglova, Karolina - Kubáč, David - Lakatos, Gergely - Grivalsky, Tomas - Caporgno, Martin Pablo - Manoel, Joao Artur Camara - Kopecky, Jiri - Masojídek, Jiří - Hrouzek, Pavel
PY  - 2020
TI  - Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes
JF  - Journal of Agricultural and Food Chemistry
VL  - 68
IS  - 6
SP  - 1654-1665
EP  - 1654-1665
PB  - American Chemical Society
SN  - 00218561
KW  - Chlorella
KW  - microalgae
KW  - selenium
KW  - selenomethionine
KW  - selenocysteine
KW  - methylselenocysteine
KW  - phototrophic cultivation
KW  - heterotrophic cultivation
UR  - https://pubs.acs.org/doi/10.1021/acs.jafc.9b06196
L2  - https://pubs.acs.org/doi/10.1021/acs.jafc.9b06196
N2  - Microalgae accumulate bioavailable selenium-containing amino acids (Se-AAs), and these are useful as a food supplement. While this accumulation has been studied in phototrophic algal cultures, little data exists for heterotrophic cultures. We have determined the Se-AAs content, selenium/sulfur (Se/S) substitution rates, and overall Se accumulation balance in photo-and heterotrophic Chlorella cultures. Laboratory trials revealed that heterotrophic cultures tolerate Se doses, similar to 8-fold higher compared to phototrophic cultures, resulting in a similar to 2-3-fold higher Se-AAs content. In large-scale experiments, both cultivation regimes provided comparable Se-AAs content. Outdoor phototrophic cultures accumulated up to 400 mu g g(-1) of total Se-AAs and exhibited a high level of Se/S substitution (5-10%) with 30-60% organic/total Se embedded in the biomass. A slightly higher content of Se-AAs and ratio of Se/S substitution was obtained for a heterotrophic culture in pilot-scale fermentors. The data presented here shows that heterotrophic Chlorella cultures provide an alternative for Se-enriched biomass production and provides information on Se-AAs content and speciation in different cultivation regimes.
ER  -

MYLENKO, Mykola, Dai Long VU, Jan KUTA, Karolina RANGLOVA, David KUBÁČ, Gergely LAKATOS, Tomas GRIVALSKY, Martin Pablo CAPORGNO, Joao Artur Camara MANOEL, Jiri KOPECKY, Jiří MASOJÍDEK and Pavel HROUZEK. Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes. \textit{Journal of Agricultural and Food Chemistry}. Washington, D.C.: American Chemical Society, 2020, vol.~68, No~6, p.~1654-1665. ISSN~0021-8561. Available from: https://dx.doi.org/10.1021/acs.jafc.9b06196.

Detailed Information on Publication Record