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@inproceedings{1672248,
author = {Plöschner, Martin and Tyc, Tomáš and Čižmár, Tomáš},
address = {BELLINGHAM},
booktitle = {MICRO+NANO MATERIALS, DEVICES, AND SYSTEMS},
doi = {http://dx.doi.org/10.1117/12.2202355},
editor = {Eggleton, B. J.; Palomba, S.},
keywords = {multimode optical fibre; digital holography; fluorescence microscopy; micro-endoscopy; wavefront shaping},
howpublished = {elektronická verze "online"},
language = {eng},
location = {BELLINGHAM},
isbn = {978-1-62841-890-3},
pages = {1-6},
publisher = {SPIE-INT SOC OPTICAL ENGINEERING},
title = {Multimode fibre: a pathway towards deep tissue fluorescence microscopy},
url = {https://doi.org/10.1117/12.2202355},
year = {2015}
}
TY - JOUR
ID - 1672248
AU - Plöschner, Martin - Tyc, Tomáš - Čižmár, Tomáš
PY - 2015
TI - Multimode fibre: a pathway towards deep tissue fluorescence microscopy
PB - SPIE-INT SOC OPTICAL ENGINEERING
CY - BELLINGHAM
SN - 9781628418903
KW - multimode optical fibre
KW - digital holography
KW - fluorescence microscopy
KW - micro-endoscopy
KW - wavefront shaping
UR - https://doi.org/10.1117/12.2202355
L2 - https://doi.org/10.1117/12.2202355
N2 - Fluorescence microscopy has emerged as a pivotal platform for imaging in the life sciences. In recent years, the overwhelming success of its different modalities has been accompanied by various efforts to carry out imaging deeper inside living tissues. A key challenge of these efforts is to overcome scattering and absorption of light in such environments. Multiple strategies (e.g. multi-photon, wavefront correction techniques) extended the penetration depth to the current state-of-the-art of about 1000 mu m at the resolution of approximately 1 mu m. The only viable strategy for imaging deeper than this is by employing a fibre bundle based endoscope. However, such devices lack resolution and have a significant footprint (1mm in diameter), which prohibits their use in studies involving tissues deep in live animals. We have recently demonstrated a radically new approach that delivers the light in/out of place of interest through an extremely thin (tens of microns in diameter) cylindrical glass tube called a multimode optical fibre (MMF). Not only is this type of delivery much less invasive compared to fibre bundle technology, it also enables higher resolution and has the ability to image at any plane behind the fibre without any auxiliary optics. The two most important limitations of this exciting technology are (i) the lack of bending flexibility and (ii) high demands on computational power, making the performance of such systems slow. We will discuss how to overcome these limitations.
ER -
PLÖSCHNER, Martin, Tomáš TYC and Tomáš ČIŽMÁR. Multimode fibre: a pathway towards deep tissue fluorescence microscopy. Online. In Eggleton, B. J.; Palomba, S. \textit{MICRO+NANO MATERIALS, DEVICES, AND SYSTEMS}. BELLINGHAM: SPIE-INT SOC OPTICAL ENGINEERING, 2015, p.~1-6. ISBN~978-1-62841-890-3. Available from: https://dx.doi.org/10.1117/12.2202355.
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