Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative
Stress Followed by Processes of Autophagy and Programmed Cell
Death

J 2020

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

BALAZOVA, Ludmila, Matej BALAZ and Petr BABULA

Basic information

Original name

Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death

Authors

BALAZOVA, Ludmila (703 Slovakia, guarantor), Matej BALAZ (703 Slovakia) and Petr BABULA (203 Czech Republic, belonging to the institution)

Edition

Nanomaterials, Basel, MDPI, 2020, 2079-4991

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

21001 Nano-materials

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.076

RIV identification code

RIV/00216224:14110/20:00116112

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/nano10061066

UT WoS

000551049800001

Keywords in English

BY-2 cells; ZnO nanoparticles; oxidative stress; autophagy; programmed cell death; phytotoxicity

Abstract

V originále

Nanomaterials, including zinc oxide nanoparticles (ZnO NPs), have a great application potential in many fields, such as medicine, the textile industry, electronics, and cosmetics. Their impact on the environment must be carefully investigated and specified due to their wide range of application. However, the amount of data on possible negative effects of ZnO NPs on plants at the cellular level are still insufficient. Thus, we focused on the effect of ZnO NPs on tobacco BY-2 cells, i.e., a widely accepted plant cell model. Adverse effects of ZnO NPs on both growth and biochemical parameters were observed. In addition, reactive oxygen and nitrogen species visualizations confirmed that ZnO NPs may induce oxidative stress. All these changes were associated with the lipid peroxidation and changes in the plasma membrane integrity, which together with endoplasmatic reticulum and mitochondrial dysfunction led to autophagy and programmed cell death. The present study demonstrates that the phytotoxic effect of ZnO NPs on the BY-2 cells is very complex and needs further investigation.

Citovat

BALAZOVA, Ludmila, Matej BALAZ and Petr BABULA. Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death. Nanomaterials. Basel: MDPI, 2020, vol. 10, No 6, p. 1-25. ISSN 2079-4991. Available from: https://dx.doi.org/10.3390/nano10061066.

@article{1672839,
   author = {Balazova, Ludmila and Balaz, Matej and Babula, Petr},
   article_location = {Basel},
   article_number = {6},
   doi = {http://dx.doi.org/10.3390/nano10061066},
   keywords = {BY-2 cells; ZnO nanoparticles; oxidative stress; autophagy; programmed cell death; phytotoxicity},
   language = {eng},
   issn = {2079-4991},
   journal = {Nanomaterials},
   title = {Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death},
   url = {https://www.mdpi.com/2079-4991/10/6/1066},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1672839
AU  - Balazova, Ludmila - Balaz, Matej - Babula, Petr
PY  - 2020
TI  - Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death
JF  - Nanomaterials
VL  - 10
IS  - 6
SP  - 1-25
EP  - 1-25
PB  - MDPI
SN  - 20794991
KW  - BY-2 cells
KW  - ZnO nanoparticles
KW  - oxidative stress
KW  - autophagy
KW  - programmed cell death
KW  - phytotoxicity
UR  - https://www.mdpi.com/2079-4991/10/6/1066
L2  - https://www.mdpi.com/2079-4991/10/6/1066
N2  - Nanomaterials, including zinc oxide nanoparticles (ZnO NPs), have a great application potential in many fields, such as medicine, the textile industry, electronics, and cosmetics. Their impact on the environment must be carefully investigated and specified due to their wide range of application. However, the amount of data on possible negative effects of ZnO NPs on plants at the cellular level are still insufficient. Thus, we focused on the effect of ZnO NPs on tobacco BY-2 cells, i.e., a widely accepted plant cell model. Adverse effects of ZnO NPs on both growth and biochemical parameters were observed. In addition, reactive oxygen and nitrogen species visualizations confirmed that ZnO NPs may induce oxidative stress. All these changes were associated with the lipid peroxidation and changes in the plasma membrane integrity, which together with endoplasmatic reticulum and mitochondrial dysfunction led to autophagy and programmed cell death. The present study demonstrates that the phytotoxic effect of ZnO NPs on the BY-2 cells is very complex and needs further investigation.
ER  -

BALAZOVA, Ludmila, Matej BALAZ and Petr BABULA. Zinc Oxide Nanoparticles Damage Tobacco BY-2 Cells by Oxidative Stress Followed by Processes of Autophagy and Programmed Cell Death. \textit{Nanomaterials}. Basel: MDPI, 2020, vol.~10, No~6, p.~1-25. ISSN~2079-4991. Available from: https://dx.doi.org/10.3390/nano10061066.

Detailed Information on Publication Record