Interleukin Gene Variability and Periodontal Bacteria in
Patients with Generalized Aggressive Form of Periodontitis

J 2020

Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

BOŘILOVÁ LINHARTOVÁ, Petra, Zdeněk DANĚK, Tereza DEISSOVÁ, Filip HROMČÍK, Břetislav LIPOVÝ et. al.

Basic information

Original name

Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis

Authors

BOŘILOVÁ LINHARTOVÁ, Petra (203 Czech Republic, belonging to the institution), Zdeněk DANĚK (203 Czech Republic, guarantor, belonging to the institution), Tereza DEISSOVÁ (203 Czech Republic, belonging to the institution), Filip HROMČÍK (203 Czech Republic, belonging to the institution), Břetislav LIPOVÝ (203 Czech Republic, belonging to the institution), Dávid SZÁRAZ (203 Czech Republic), Július JÁNOŠ (703 Slovakia, belonging to the institution), Antonín FASSMANN (203 Czech Republic), Jirina BARTOVA (203 Czech Republic), Ivo DRIZHAL (203 Czech Republic) and Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic, belonging to the institution)

Edition

International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.923

RIV identification code

RIV/00216224:14110/20:00116132

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/ijms21134728

UT WoS

000550282500001

Keywords in English

aggressive periodontitis; oral bacteria; inflammation; interleukin; polymorphism; genetic predisposition

Abstract

V originále

Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms inIL-1gene cluster,IL-6and its receptor,IL-10,IL-17A, andIL-18were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphismIL-10-1087 A/G (rs1800896) and specificIL-10haplotypes were associated with the development of the disease (p< 0.05,P-corr> 0.05). Four bacterial species occurred more frequently in AgP than in controls (p< 0.01,P-corr< 0.05). Elevated IL-10 levels were found in AgP patients, carriers ofIL-10-1087GG genotype, and PBMCs stimulated by periodontal bacteria (p< 0.05,P-corr> 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression ofIL-10and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development.

Links

MUNI/A/1428/2019, interní kód MU

Name: Onemocnění dutiny ústní – etiopatogeneze, diagnostika a léčba

Investor: Masaryk University, Category A

NU20-08-00205, research and development project

Name: Molekulární etiopatogeneze apikální periodontitidy a odontogenních cyst

Investor: Ministry of Health of the CR, Subprogram 1 - standard

ROZV/28/LF4/2020, interní kód MU

Name: Analýza genetických markerů a orálního mikrobiomu ve vztahu k vybraným onemocněním orofaciálního systému

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

BOŘILOVÁ LINHARTOVÁ, Petra, Zdeněk DANĚK, Tereza DEISSOVÁ, Filip HROMČÍK, Břetislav LIPOVÝ, Dávid SZÁRAZ, Július JÁNOŠ, Antonín FASSMANN, Jirina BARTOVA, Ivo DRIZHAL and Lydie IZAKOVIČOVÁ HOLLÁ. Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis. International Journal of Molecular Sciences. Basel: MDPI, 2020, vol. 21, No 13, p. 1-24. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21134728.

@article{1673161,
   author = {Bořilová Linhartová, Petra and Daněk, Zdeněk and Deissová, Tereza and Hromčík, Filip and Lipový, Břetislav and Száraz, Dávid and Jánoš, Július and Fassmann, Antonín and Bartova, Jirina and Drizhal, Ivo and Izakovičová Hollá, Lydie},
   article_location = {Basel},
   article_number = {13},
   doi = {http://dx.doi.org/10.3390/ijms21134728},
   keywords = {aggressive periodontitis; oral bacteria; inflammation; interleukin; polymorphism; genetic predisposition},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis},
   url = {https://www.mdpi.com/1422-0067/21/13/4728},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1673161
AU  - Bořilová Linhartová, Petra - Daněk, Zdeněk - Deissová, Tereza - Hromčík, Filip - Lipový, Břetislav - Száraz, Dávid - Jánoš, Július - Fassmann, Antonín - Bartova, Jirina - Drizhal, Ivo - Izakovičová Hollá, Lydie
PY  - 2020
TI  - Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis
JF  - International Journal of Molecular Sciences
VL  - 21
IS  - 13
SP  - 1-24
EP  - 1-24
PB  - MDPI
SN  - 14220067
KW  - aggressive periodontitis
KW  - oral bacteria
KW  - inflammation
KW  - interleukin
KW  - polymorphism
KW  - genetic predisposition
UR  - https://www.mdpi.com/1422-0067/21/13/4728
L2  - https://www.mdpi.com/1422-0067/21/13/4728
N2  - Host genetic predispositions to dysregulated immune response can influence the development of the aggressive form of periodontitis (AgP) through susceptibility to oral dysbiosis and subsequent host-microbe interaction. This case-control study aimed to perform a multilocus analysis of functional variants in selected interleukin (IL) genes in patients with the generalized form of AgP in a homogenous population. Twelve polymorphisms inIL-1gene cluster,IL-6and its receptor,IL-10,IL-17A, andIL-18were determined in 91 AgP patients and 210 controls. Analysis of seven selected periodontal bacteria in subgingival sulci/pockets was performed with a commercial DNA-microarray kit in a subgroup of 76 individuals. The pilot in vitro study included stimulation of peripheral blood monocytes (PBMC) from 20 individuals with periodontal bacteria and measurement of IL-10 levels using the Luminex method. Only the unctional polymorphismIL-10-1087 A/G (rs1800896) and specificIL-10haplotypes were associated with the development of the disease (p< 0.05,P-corr> 0.05). Four bacterial species occurred more frequently in AgP than in controls (p< 0.01,P-corr< 0.05). Elevated IL-10 levels were found in AgP patients, carriers ofIL-10-1087GG genotype, and PBMCs stimulated by periodontal bacteria (p< 0.05,P-corr> 0.05). We therefore conclude that a combination of genetic predisposition to the altered expression ofIL-10and the presence of specific periodontal bacteria may contribute to Th1/Th2 balance disruption and AgP development.
ER  -

BOŘILOVÁ LINHARTOVÁ, Petra, Zdeněk DANĚK, Tereza DEISSOVÁ, Filip HROMČÍK, Břetislav LIPOVÝ, Dávid SZÁRAZ, Július JÁNOŠ, Antonín FASSMANN, Jirina BARTOVA, Ivo DRIZHAL and Lydie IZAKOVIČOVÁ HOLLÁ. Interleukin Gene Variability and Periodontal Bacteria in Patients with Generalized Aggressive Form of Periodontitis. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, vol.~21, No~13, p.~1-24. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21134728.

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