Detailed Information on Publication Record
2020
Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
ROUDNICKÝ, Pavel, David POTĚŠIL, Zbyněk ZDRÁHAL, Milan GELNAR, Martin KAŠNÝ et. al.Basic information
Original name
Laser capture microdissection in combination with mass spectrometry: Approach to characterization of tissue-specific proteomes of Eudiplozoon nipponicum (Monogenea, Polyopisthocotylea)
Authors
ROUDNICKÝ, Pavel (203 Czech Republic, guarantor, belonging to the institution), David POTĚŠIL (203 Czech Republic, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Milan GELNAR (203 Czech Republic, belonging to the institution) and Martin KAŠNÝ (203 Czech Republic, belonging to the institution)
Edition
PLOS ONE, San Francisco, Public Library of Science, 2020, 1932-6203
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10613 Zoology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 3.240
RIV identification code
RIV/00216224:14310/20:00114261
Organization unit
Faculty of Science
UT WoS
000542759600012
Keywords in English
helminths; parasite; peptidase; inhibitor; laser microdissection; mass spectrometry
Tags
International impact, Reviewed
Změněno: 15/10/2024 09:24, Ing. Martina Blahová
Abstract
V originále
Eudiplozoon nipponicum (Goto, 1891) is a hematophagous monogenean ectoparasite which inhabits the gills of the common carp (Cyprinus carpio). Heavy infestation can lead to anemia and in conjunction with secondary bacterial infections cause poor health and eventual death of the host. This study is based on an innovative approach to protein localization which has never been used in parasitology before. Using laser capture microdissection, we dissected particular areas of the parasite body without contaminating the samples by surrounding tissue and in combination with analysis by mass spectrometry obtained tissue-specific proteomes of tegument, intestine, and parenchyma of our model organism, E. nipponicum. We successfully verified the presence of certain functional proteins (e.g. cathepsin L) in tissues where their presence was expected (intestine) and confirmed that there were no traces of these proteins in other tissues (tegument and parenchyma). Additionally, we identified a total of 2,059 proteins, including 72 peptidases and 33 peptidase inhibitors. As expected, the greatest variety was found in the intestine and the lowest variety in the parenchyma. Our results are significant on two levels. Firstly, we demonstrated that one can localize all proteins in one analysis and without using laboratory animals (antibodies for immunolocalization of single proteins). Secondly, this study offers the first complex proteomic data on not only the E. nipponicum but within the whole class of Monogenea, which was from this point of view until recently neglected.
Links
| GAP506/12/1258, research and development project |
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| GBP505/12/G112, research and development project |
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| LM2018127, research and development project |
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| LM2018140, research and development project |
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| LQ1601, research and development project |
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| MUNI/A/0918/2018, interní kód MU |
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| 90127, large research infrastructures |
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| 90132, large research infrastructures |
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