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@article{1673419,
author = {Víšová, Ivana and Smolková, Barbora and Uzhytchak, Mariia and Vrabcová, Markéta and Chafai, Djamel Eddine and Houska, Milan and Pastucha, Matěj and Skládal, Petr and Farka, Zdeněk and Dejneka, Alexandr and VaisocherováandLísalová, Hana},
article_location = {Basel},
article_number = {8},
doi = {http://dx.doi.org/10.3390/biom10081146},
keywords = {zwitterionic material; cell mechanotransduction; cell signaling; functional biointerfaces; antifouling polymer brushes; surface modification},
language = {eng},
issn = {2218-273X},
journal = {Biomolecules},
title = {Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery},
url = {https://www.mdpi.com/2218-273X/10/8/1146},
volume = {10},
year = {2020}
}
TY - JOUR
ID - 1673419
AU - Víšová, Ivana - Smolková, Barbora - Uzhytchak, Mariia - Vrabcová, Markéta - Chafai, Djamel Eddine - Houska, Milan - Pastucha, Matěj - Skládal, Petr - Farka, Zdeněk - Dejneka, Alexandr - Vaisocherová-Lísalová, Hana
PY - 2020
TI - Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery
JF - Biomolecules
VL - 10
IS - 8
SP - 1-14
EP - 1-14
PB - MDPI
SN - 2218273X
KW - zwitterionic material
KW - cell mechanotransduction
KW - cell signaling
KW - functional biointerfaces
KW - antifouling polymer brushes
KW - surface modification
UR - https://www.mdpi.com/2218-273X/10/8/1146
L2 - https://www.mdpi.com/2218-273X/10/8/1146
N2 - Cells are continuously sensing their microenvironment and subsequently respond to different physicochemical cues by the activation or inhibition of different signaling pathways. To study a very complex cellular response, it is necessary to diminish background environmental influences and highlight the particular event. However, surface-driven nonspecific interactions of the abundant biomolecules from the environment influence the targeted cell response significantly. Yes-associated protein (YAP) translocation may serve as a marker of human hepatocellular carcinoma (Huh7) cell responses to the extracellular matrix and surface-mediated stresses. Here, we propose a platform of tunable functionable antifouling poly(carboxybetain) (pCB)-based brushes to achieve a molecularly clean background for studying arginine, glycine, and aspartic acid (RGD)-induced YAP-connected mechanotransduction. Using two different sets of RGD-functionalized zwitterionic antifouling coatings with varying compositions of the antifouling layer, a clear correlation of YAP distribution with RGD functionalization concentrations was observed. On the other hand, commonly used surface passivation by the oligo(ethylene glycol)-based self-assembled monolayer (SAM) shows no potential to induce dependency of the YAP distribution on RGD concentrations. The results indicate that the antifouling background is a crucial component of surface-based cellular response studies, and pCB-based zwitterionic antifouling brush architectures may serve as a potential next-generation easily functionable surface platform for the monitoring and quantification of cellular processes.
ER -
VÍŠOVÁ, Ivana, Barbora SMOLKOVÁ, Mariia UZHYTCHAK, Markéta VRABCOVÁ, Djamel Eddine CHAFAI, Milan HOUSKA, Matěj PASTUCHA, Petr SKLÁDAL, Zdeněk FARKA, Alexandr DEJNEKA and Hana VAISOCHEROVÁ-LÍSALOVÁ. Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery. \textit{Biomolecules}. Basel: MDPI, 2020, vol.~10, No~8, p.~1-14. ISSN~2218-273X. Available from: https://dx.doi.org/10.3390/biom10081146.
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