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@article{1674004,
author = {Prochazkova, Jirina and Slavik, Josef and Bouchal, Jan and Levkova, Monika and Huskova, Zlata and Ehrmann, Jiri and Ovesná, Petra and Kolar, Zdenek and Skalicky, Pavel and Strakova, Nicol and Zapletal, Ondrej and Kozubik, Alois and Hofmanova, Jirina and Vondracek, Jan and Machala, Miroslav},
article_location = {AMSTERDAM},
article_number = {9},
doi = {http://dx.doi.org/10.1016/j.bbalip.2020.158742},
keywords = {Sphingolipid metabolism; Colon adenocarcinoma; Lactosylceramide; B4GALTs; EPCAM-positive cells},
language = {eng},
issn = {1388-1981},
journal = {BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS},
title = {Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors},
url = {https://www.sciencedirect.com/science/article/pii/S1388198120301347?via%3Dihub},
volume = {1865},
year = {2020}
}
TY - JOUR
ID - 1674004
AU - Prochazkova, Jirina - Slavik, Josef - Bouchal, Jan - Levkova, Monika - Huskova, Zlata - Ehrmann, Jiri - Ovesná, Petra - Kolar, Zdenek - Skalicky, Pavel - Strakova, Nicol - Zapletal, Ondrej - Kozubik, Alois - Hofmanova, Jirina - Vondracek, Jan - Machala, Miroslav
PY - 2020
TI - Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors
JF - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
VL - 1865
IS - 9
SP - 1-11
EP - 1-11
PB - ELSEVIER
SN - 13881981
KW - Sphingolipid metabolism
KW - Colon adenocarcinoma
KW - Lactosylceramide
KW - B4GALTs
KW - EPCAM-positive cells
UR - https://www.sciencedirect.com/science/article/pii/S1388198120301347?via%3Dihub
L2 - https://www.sciencedirect.com/science/article/pii/S1388198120301347?via%3Dihub
N2 - Metabolic reprogramming leading to alterations in lipid metabolism and lipid-mediated signaling may contribute to colorectal cancer (CRC) development and progression. We hypothesized that a detailed description of changes in sphingolipidome of specific cellular subpopulation residing in colon cancer tissue may help to discriminate between normal and transformed colon epithelial cells. Using HPLC-mass spectrometry, we analyzed the EpCAM-positive cells isolated from tumor and adjacent non-tumor tissues of colon cancer patients, aiming to identify potential lipid biomarkers specific for CRC. We then employed RT-qPCR-based methodology in order to analyze expression of SL metabolism-related genes in the isolated EpCAM-positive tumor cells and/or in unseparated colon tumor tissues. We observed significant changes in sphingolipid (SL) species in the EpCAM-positive tumor cells, with the accumulation of lactosylceramide (LacCer) being the most prominent. B4GALT5 and B4GALT6 genes were identified as two potential gene candidates contributing to LacCer accumulation. We further identified additional genes of SL and fatty acid metabolism (e.g. SPHK1, GBA2, NEU3, GLA, FASN, PLA2G10), which were significantly altered in colon tumor tissue. The present results indicate that the EpCAM-positive cells are a major contributor to LacCer accumulation in CRC tissue, and may help to identify novel CRC-specific lipid/gene biomarkers.
ER -
PROCHAZKOVA, Jirina, Josef SLAVIK, Jan BOUCHAL, Monika LEVKOVA, Zlata HUSKOVA, Jiri EHRMANN, Petra OVESNÁ, Zdenek KOLAR, Pavel SKALICKY, Nicol STRAKOVA, Ondrej ZAPLETAL, Alois KOZUBIK, Jirina HOFMANOVA, Jan VONDRACEK and Miroslav MACHALA. Specific alterations of sphingolipid metabolism identified in EpCAM-positive cells isolated from human colon tumors. \textit{BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS}. AMSTERDAM: ELSEVIER, 2020, vol.~1865, No~9, p.~1-11. ISSN~1388-1981. Available from: https://dx.doi.org/10.1016/j.bbalip.2020.158742.
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