Loss of histone macroH2A1 in hepatocellular carcinoma cells
promotes paracrine-mediated chemoresistance and
CD4(+)CD25(+)FoxP3(+) regulatory T cells activation

J 2020

Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4(+)CD25(+)FoxP3(+) regulatory T cells activation

LO RE, Oriana; Tommaso MAZZA; Sebastiano GIALLONGO; Paola SANNA; Francesca RAPPA et. al.

Basic information

Original name

Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4(+)CD25(+)FoxP3(+) regulatory T cells activation

Authors

Edition

THERANOSTICS, LAKE HAVEN, IVYSPRING INT PUBL, 2020, 1838-7640

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

10601 Cell biology

Country of publisher

Australia

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 11.556

RIV identification code

RIV/00216224:14110/20:00116196

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.7150/thno.35045

UT WoS

000498874200028

EID Scopus

2-s2.0-85077480426

Keywords in English

hepatocellular carcinoma; histone macroH2A1; adaptive immune system; chemoresistance

Abstract

In the original language

Rationale: Loss of histone macroH2A1 induces appearance of cancer stem cells (CSCs)-like cells in hepatocellular carcinoma (HCC). How CSCs interact with the tumor microenvironment and the adaptive immune system is unclear. Methods: We screened aggressive human HCC for macroH2A1 and CD44 CSC marker expression. We also knocked down (KD) macroH2A1 in HCC cells, and performed integrated transcriptomic and secretomic analyses. Results: Human HCC showed low macroH2A1 and high CD44 expression compared to control tissues. MacroH2A1 KD CSC-like cells transferred paracrinally their chemoresistant properties to parental HCC cells. MacroH2A1 KD conditioned media transcriptionally reprogrammed parental HCC cells activated regulatory CD4(+)/CD25(+)/FoxP3(+) T cells (Tregs). Conclusions: Loss of macroH2A1 in HCC cells drives cancer stem-cell propagation and evasion from immune surveillance.

Links

LM2015091, research and development project

Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/0951/2019, interní kód MU

Name: Buněčná a molekulární biologie pro Biomedicínské vědy

Investor: Masaryk University, Category A

Cite

LO RE, Oriana; Tommaso MAZZA; Sebastiano GIALLONGO; Paola SANNA; Francesca RAPPA; Tu Vinh Luong LUONG; Giovanni VOLTI; Adéla DVOŘÁKOVÁ; Tania ROSKAMS; Matthias VAN HAELE; Emmanuel TSOCHATZIS and Manlio VINCIGUERRA. Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4(+)CD25(+)FoxP3(+) regulatory T cells activation. THERANOSTICS. LAKE HAVEN: IVYSPRING INT PUBL, 2020, vol. 10, No 2, p. 910-924. ISSN 1838-7640. Available from: https://doi.org/10.7150/thno.35045.

@article{1674038,
   author = {Lo Re, Oriana and Mazza, Tommaso and Giallongo, Sebastiano and Sanna, Paola and Rappa, Francesca and Luong, Tu Vinh Luong and Volti, Giovanni and Dvořáková, Adéla and Roskams, Tania and Van Haele, Matthias and Tsochatzis, Emmanuel and Vinciguerra, Manlio},
   article_location = {LAKE HAVEN},
   article_number = {2},
   doi = {https://doi.org/10.7150/thno.35045},
   keywords = {hepatocellular carcinoma; histone macroH2A1; adaptive immune system; chemoresistance},
   language = {eng},
   issn = {1838-7640},
   journal = {THERANOSTICS},
   title = {Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4(+)CD25(+)FoxP3(+) regulatory T cells activation},
   url = {https://www.thno.org/v10p0910.htm},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1674038
AU  - Lo Re, Oriana - Mazza, Tommaso - Giallongo, Sebastiano - Sanna, Paola - Rappa, Francesca - Luong, Tu Vinh Luong - Volti, Giovanni - Dvořáková, Adéla - Roskams, Tania - Van Haele, Matthias - Tsochatzis, Emmanuel - Vinciguerra, Manlio
PY  - 2020
TI  - Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4(+)CD25(+)FoxP3(+) regulatory T cells activation
JF  - THERANOSTICS
VL  - 10
IS  - 2
SP  - 910-924
EP  - 910-924
PB  - IVYSPRING INT PUBL
SN  - 18387640
KW  - hepatocellular carcinoma
KW  - histone macroH2A1
KW  - adaptive immune system
KW  - chemoresistance
UR  - https://www.thno.org/v10p0910.htm
L2  - https://www.thno.org/v10p0910.htm
N2  - Rationale: Loss of histone macroH2A1 induces appearance of cancer stem cells (CSCs)-like cells in hepatocellular carcinoma (HCC). How CSCs interact with the tumor microenvironment and the adaptive immune system is unclear. Methods: We screened aggressive human HCC for macroH2A1 and CD44 CSC marker expression. We also knocked down (KD) macroH2A1 in HCC cells, and performed integrated transcriptomic and secretomic analyses. Results: Human HCC showed low macroH2A1 and high CD44 expression compared to control tissues. MacroH2A1 KD CSC-like cells transferred paracrinally their chemoresistant properties to parental HCC cells. MacroH2A1 KD conditioned media transcriptionally reprogrammed parental HCC cells activated regulatory CD4(+)/CD25(+)/FoxP3(+) T cells (Tregs). Conclusions: Loss of macroH2A1 in HCC cells drives cancer stem-cell propagation and evasion from immune surveillance.
ER  -

LO RE, Oriana; Tommaso MAZZA; Sebastiano GIALLONGO; Paola SANNA; Francesca RAPPA; Tu Vinh Luong LUONG; Giovanni VOLTI; Adéla DVOŘÁKOVÁ; Tania ROSKAMS; Matthias VAN HAELE; Emmanuel TSOCHATZIS and Manlio VINCIGUERRA. Loss of histone macroH2A1 in hepatocellular carcinoma cells promotes paracrine-mediated chemoresistance and CD4(+)CD25(+)FoxP3(+) regulatory T cells activation. \textit{THERANOSTICS}. LAKE HAVEN: IVYSPRING INT PUBL, 2020, vol.~10, No~2, p.~910-924. ISSN~1838-7640. Available from: https://doi.org/10.7150/thno.35045.

Přehled o publikaci