Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC
Behavior and Properties

J 2020

Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties

JAUKOVIC, Aleksandra, Desislava ABADJIEVA, Drenka TRIVANOVIC, Elena STOYANOVA, Milena KOSTADINOVA et. al.

Basic information

Original name

Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties

Authors

JAUKOVIC, Aleksandra (688 Serbia), Desislava ABADJIEVA (100 Bulgaria), Drenka TRIVANOVIC (688 Serbia), Elena STOYANOVA (100 Bulgaria), Milena KOSTADINOVA (100 Bulgaria), Shina PASHOVA (100 Bulgaria), Snejana KESTENDJIEVA (100 Bulgaria), Tamara KUKOLJ (688 Serbia), Michal JEŠETA (203 Czech Republic, belonging to the institution), Elena KISTANOVA (100 Bulgaria) and Milena MOURDJEVA (100 Bulgaria, guarantor)

Edition

Stem Cell Reviews and Reports, NEW YORK, SPRINGER, 2020, 2629-3269

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.739

RIV identification code

RIV/00216224:14110/20:00116197

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s12015-020-10006-9

UT WoS

000549663600001

Keywords in English

Mesenchymal stem cells; 3D spheroids microenvironment; Morphological changes; Cell survival; Energy metabolism; Epigenetic regulation; Improved functionality

Abstract

V originále

Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.

Citovat

JAUKOVIC, Aleksandra, Desislava ABADJIEVA, Drenka TRIVANOVIC, Elena STOYANOVA, Milena KOSTADINOVA, Shina PASHOVA, Snejana KESTENDJIEVA, Tamara KUKOLJ, Michal JEŠETA, Elena KISTANOVA and Milena MOURDJEVA. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. Stem Cell Reviews and Reports. NEW YORK: SPRINGER, 2020, vol. 16, No 5, p. 853-875. ISSN 2629-3269. Available from: https://dx.doi.org/10.1007/s12015-020-10006-9.

@article{1674042,
   author = {Jaukovic, Aleksandra and Abadjieva, Desislava and Trivanovic, Drenka and Stoyanova, Elena and Kostadinova, Milena and Pashova, Shina and Kestendjieva, Snejana and Kukolj, Tamara and Ješeta, Michal and Kistanova, Elena and Mourdjeva, Milena},
   article_location = {NEW YORK},
   article_number = {5},
   doi = {http://dx.doi.org/10.1007/s12015-020-10006-9},
   keywords = {Mesenchymal stem cells; 3D spheroids microenvironment; Morphological changes; Cell survival; Energy metabolism; Epigenetic regulation; Improved functionality},
   language = {eng},
   issn = {2629-3269},
   journal = {Stem Cell Reviews and Reports},
   title = {Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties},
   url = {https://link.springer.com/article/10.1007%2Fs12015-020-10006-9},
   volume = {16},
   year = {2020}
}

TY  - JOUR
ID  - 1674042
AU  - Jaukovic, Aleksandra - Abadjieva, Desislava - Trivanovic, Drenka - Stoyanova, Elena - Kostadinova, Milena - Pashova, Shina - Kestendjieva, Snejana - Kukolj, Tamara - Ješeta, Michal - Kistanova, Elena - Mourdjeva, Milena
PY  - 2020
TI  - Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties
JF  - Stem Cell Reviews and Reports
VL  - 16
IS  - 5
SP  - 853-875
EP  - 853-875
PB  - SPRINGER
SN  - 26293269
KW  - Mesenchymal stem cells
KW  - 3D spheroids microenvironment
KW  - Morphological changes
KW  - Cell survival
KW  - Energy metabolism
KW  - Epigenetic regulation
KW  - Improved functionality
UR  - https://link.springer.com/article/10.1007%2Fs12015-020-10006-9
L2  - https://link.springer.com/article/10.1007%2Fs12015-020-10006-9
N2  - Mesenchymal stem cells (MSC) have been considered the promising candidates for the regenerative and personalized medicine due to their self-renewal potential, multilineage differentiation and immunomodulatory capacity. Although these properties have encouraged profound MSC studies in recent years, the majority of research has been based on standard 2D culture utilization. The opportunity to resemble in vivo characteristics of cells native niche has been provided by implementation of 3D culturing models such as MSC spheroid formation assesed through cells self-assembling. In this review, we address the current literature on physical and biochemical features of 3D MSC spheroid microenvironment and their impact on MSC properties and behaviors. Starting with the reduction in the cells' dimensions and volume due to the changes in adhesion molecules expression and cytoskeletal proteins rearrangement resembling native conditions, through the microenvironment shifts in oxygen, nutrients and metabolites gradients and demands, we focus on distinctive and beneficial features of MSC in spheroids compared to cells cultured in 2D conditions. By summarizing the data for 3D MSC spheroids regarding cell survival, pluripotency, differentiation, immunomodulatory activities and potential to affect tumor cells growth we highlighted advantages and perspectives of MSC spheroids use in regenerative medicine. Further detailed analyses are needed to deepen our understanding of mechanisms responsible for modified MSC behavior in spheroids and to set future directions for MSC clinical application.
ER  -

JAUKOVIC, Aleksandra, Desislava ABADJIEVA, Drenka TRIVANOVIC, Elena STOYANOVA, Milena KOSTADINOVA, Shina PASHOVA, Snejana KESTENDJIEVA, Tamara KUKOLJ, Michal JEŠETA, Elena KISTANOVA and Milena MOURDJEVA. Specificity of 3D MSC Spheroids Microenvironment: Impact on MSC Behavior and Properties. \textit{Stem Cell Reviews and Reports}. NEW YORK: SPRINGER, 2020, vol.~16, No~5, p.~853-875. ISSN~2629-3269. Available from: https://dx.doi.org/10.1007/s12015-020-10006-9.

Detailed Information on Publication Record