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@article{1674060,
author = {Kulus, M. and Kranc, W. and SujkaandKordowska, P. and Celichowski, P. and Konwerska, A. and Jankowski, M. and Ješeta, Michal and Skowronski, M. T. and PiotrowskaandKempisty, H. and Bukowska, D. and Zabel, M. and Bruska, M. and Mozdziak, P. and Kempisty, B. and Antosik, P.},
article_location = {New York},
article_number = {6},
doi = {http://dx.doi.org/10.1007/s00418-020-01860-2},
keywords = {Pig; Ovarian follicle; Granulosa cells; Primary culture; Microarray},
language = {eng},
issn = {0948-6143},
journal = {Histochemistry and Cell Biology},
title = {Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture},
url = {https://link.springer.com/content/pdf/10.1007/s00418-020-01860-2.pdf},
volume = {153},
year = {2020}
}
TY - JOUR
ID - 1674060
AU - Kulus, M. - Kranc, W. - Sujka-Kordowska, P. - Celichowski, P. - Konwerska, A. - Jankowski, M. - Ješeta, Michal - Skowronski, M. T. - Piotrowska-Kempisty, H. - Bukowska, D. - Zabel, M. - Bruska, M. - Mozdziak, P. - Kempisty, B. - Antosik, P.
PY - 2020
TI - Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture
JF - Histochemistry and Cell Biology
VL - 153
IS - 6
SP - 397-412
EP - 397-412
PB - Springer
SN - 09486143
KW - Pig
KW - Ovarian follicle
KW - Granulosa cells
KW - Primary culture
KW - Microarray
UR - https://link.springer.com/content/pdf/10.1007/s00418-020-01860-2.pdf
L2 - https://link.springer.com/content/pdf/10.1007/s00418-020-01860-2.pdf
N2 - The primary function of ovarian granulosa cells (GCs) is the support of oocytes during maturation and development. Molecular analyses of granulosa cell-associated processes, leading to improvement of understanding of the cell cycle events during the formation of ovarian follicles (folliculogenesis), may be key to improve the in vitro fertilization procedures. Primary in vitro culture of porcine GCs was employed to examine the changes in the transcriptomic profile of genes belonging to "cell cycle", "cell division", "cell cycle process", "cell cycle phase transition", "cell cycle G1/S phase transition", "cell cycle G2/M phase transition" and "cell cycle checkpoint" ontology groups. During the analysis, microarrays were employed to study the transcriptome of GCs, analyzing the total RNA of cells from specific periods of in vitro cultures. This research was based on material obtained from 40 landrace gilts of similar weight, age and the same living conditions. RNA was isolated at specific timeframes: before the culture was established (0 h) and after 48 h, 96 h and 144 h in vitro. Out of 133 differentially expressed genes, we chose the 10 most up-regulated (SFRP2,PDPN,PDE3A,FGFR2,PLK2,THBS1,ETS1,LIF,ANXA1,TGFB1) and the 10 most downregulated (IGF1,NCAPD2,CABLES1,H1FOO,NEK2,PPAT,TXNIP,NUP210,RGS2andCCNE2). Some of these genes known to play key roles in the regulation of correct cell cycle passage (up-regulated SFRP2, PDE3A, PLK2, LIF and down-regulated CCNE2, TXNIP, NEK2). The data obtained provide a potential reference for studies on the process of mammalian folliculogenesis, as well as suggests possible new genetic markers for cell cycle progress in in vitro cultured porcine granulosa cells.
ER -
KULUS, M., W. KRANC, P. SUJKA-KORDOWSKA, P. CELICHOWSKI, A. KONWERSKA, M. JANKOWSKI, Michal JEŠETA, M. T. SKOWRONSKI, H. PIOTROWSKA-KEMPISTY, D. BUKOWSKA, M. ZABEL, M. BRUSKA, P. MOZDZIAK, B. KEMPISTY and P. ANTOSIK. Transcriptomic analysis of expression of genes regulating cell cycle progression in porcine ovarian granulosa cells during short-term in vitro primary culture. \textit{Histochemistry and Cell Biology}. New York: Springer, 2020, vol.~153, No~6, p.~397-412. ISSN~0948-6143. Available from: https://dx.doi.org/10.1007/s00418-020-01860-2.
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