Detailed Information on Publication Record
2020
Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR
ROBEŠOVÁ, Blanka, Marie DRNCOVA, Adam FOLTA, Marta KREJČÍ, František FOLBER et. al.Basic information
Original name
Donor-derived DNA variability in fingernails of acute myeloid leukemia patients after allogeneic hematopoietic stem cell transplantation detected by direct PCR
Authors
ROBEŠOVÁ, Blanka (203 Czech Republic), Marie DRNCOVA (203 Czech Republic), Adam FOLTA (203 Czech Republic), Marta KREJČÍ (203 Czech Republic, belonging to the institution), František FOLBER (203 Czech Republic, belonging to the institution), Zuzana ŠUSTKOVÁ (203 Czech Republic, belonging to the institution), Jiří MAYER (203 Czech Republic, belonging to the institution) and Ivana JEŽÍŠKOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Bone Marrow Transplantation, London, Nature Publishing Group, 2020, 0268-3369
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30205 Hematology
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.483
RIV identification code
RIV/00216224:14110/20:00116201
Organization unit
Faculty of Medicine
UT WoS
000531193900001
Keywords in English
acute myeloid leukemia; Donor-derived DNA; fingernails; stem cell transplantation
Tags
International impact, Reviewed
Změněno: 22/9/2020 12:06, Mgr. Tereza Miškechová
Abstract
V originále
Human fingernails are an important material used to identify individuals in forensic medicine as well as a source of DNA for genetic analyses in different fields of study. Nail clippings have many advantages, such as stability and suitability for long-term storage, easy non-invasive sampling and simple transport. On the other hand, the structure of a fingernail (presence of keratinized cells) requires a more complicated DNA extraction method than DNA extraction from blood cells. Therefore, it is advantageous to use a direct PCR method that does not require a DNA isolation step. This method was used on fingernails for the first time by Tie et al., and by Ottens et al. The benefits of the method are simplicity, reducing the possibility of contamination and time- and cost-saving. We used this innovative method for donor-derived DNA analysis on the fingernails of 30 acute myeloid patients (AML) with long-lasting complete donor chimerism in their peripheral blood after the first allogeneic hematopoietic stem cell transplantation (allo-HSCT).