Two combinatorial patterns of telomerase histone marks in
plants with canonical and non-canonical telomere repeats

J 2020

Two combinatorial patterns of telomerase histone marks in plants with canonical and non-canonical telomere repeats

ADAMUSOVÁ, Kateřina, Solmaz KOSHRAVI, Satoru FUJIMOTO, Andreas HOUBEN, Sachihiro MATSUNAGA et. al.

Basic information

Original name

Two combinatorial patterns of telomerase histone marks in plants with canonical and non-canonical telomere repeats

Authors

ADAMUSOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Solmaz KOSHRAVI, Satoru FUJIMOTO, Andreas HOUBEN (276 Germany), Sachihiro MATSUNAGA, Jiří FAJKUS (203 Czech Republic, belonging to the institution) and Miloslava FOJTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

The Plant Journal, Blackwell Science, 2020, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.417

RIV identification code

RIV/00216224:14740/20:00116206

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/tpj.14653

UT WoS

000506712300001

Keywords in English

Arabidopsis thaliana; chromatin; epigenetics; histone modifications; Nicotiana; telomeres

Abstract

V originále

Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are crucial for the maintenance of genome integrity. In most plants, telomeres consist of conserved tandem repeat units comprising the TTTAGGG motif. Recently, non-canonical telomeres were described in several plants and plant taxons, including the carnivorous plant Genlisea hispidula (TTCAGG/TTTCAGG), the genus Cestrum (Solanaceae; TTTTTTAGGG), and plants from the Asparagales order with either a vertebrate-type telomere repeat TTAGGG or Allium genus-specific CTCGGTTATGGG repeat. We analyzed epigenetic modifications of telomeric histones in plants with canonical and non-canonical telomeres, and further in telomeric chromatin captured from leaves of Nicotiana benthamiana transiently transformed by telomere CRISPR-dCas9-eGFP, and of Arabidopsis thaliana stably transformed with TALE_telo C-3×GFP. Two combinatorial patterns of telomeric histone modifications were identified: (i) an Arabidopsis-like pattern (A. thaliana, G. hispidula, Genlisea nigrocaulis, Allium cepa, Narcissus pseudonarcissus, Petunia hybrida, Solanum tuberosum, Solanum lycopersicum) with telomeric histones decorated predominantly by H3K9me2; (ii) a tobacco-like pattern (Nicotiana tabacum, N. benthamiana, C. elegans) with a strong H3K27me3 signal. Our data suggest that epigenetic modifications of plant telomere-associated histones are related neither to the sequence of the telomere motif nor to the lengths of the telomeres. Nor the phylogenetic position of the species plays the role; representatives of the Solanaceae family are included in both groups. As both patterns of histone marks are compatible with fully functional telomeres in respective plants, we conclude that the described specific differences in histone marks are not critical for telomere functions.

Links

EF16_026/0008446, research and development project

Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin

LTC17077, research and development project

Name: Telomerový chromatin v rostlinách ovlivněných epigeneticky účinnými látkami

Investor: Ministry of Education, Youth and Sports of the CR, Telomeric chromatin in plants influenced by epigenetic drugs, INTER-COST

Citovat

ADAMUSOVÁ, Kateřina, Solmaz KOSHRAVI, Satoru FUJIMOTO, Andreas HOUBEN, Sachihiro MATSUNAGA, Jiří FAJKUS and Miloslava FOJTOVÁ. Two combinatorial patterns of telomerase histone marks in plants with canonical and non-canonical telomere repeats. The Plant Journal. Blackwell Science, 2020, vol. 102, No 4, p. 678-687. ISSN 0960-7412. Available from: https://dx.doi.org/10.1111/tpj.14653.

@article{1674164,
   author = {Adamusová, Kateřina and Koshravi, Solmaz and Fujimoto, Satoru and Houben, Andreas and Matsunaga, Sachihiro and Fajkus, Jiří and Fojtová, Miloslava},
   article_number = {4},
   doi = {http://dx.doi.org/10.1111/tpj.14653},
   keywords = {Arabidopsis thaliana; chromatin; epigenetics; histone modifications; Nicotiana; telomeres},
   language = {eng},
   issn = {0960-7412},
   journal = {The Plant Journal},
   title = {Two combinatorial patterns of telomerase histone marks in plants with canonical and non-canonical telomere repeats},
   url = {https://pubmed.ncbi.nlm.nih.gov/31834959/},
   volume = {102},
   year = {2020}
}

TY  - JOUR
ID  - 1674164
AU  - Adamusová, Kateřina - Koshravi, Solmaz - Fujimoto, Satoru - Houben, Andreas - Matsunaga, Sachihiro - Fajkus, Jiří - Fojtová, Miloslava
PY  - 2020
TI  - Two combinatorial patterns of telomerase histone marks in plants with canonical and non-canonical telomere repeats
JF  - The Plant Journal
VL  - 102
IS  - 4
SP  - 678-687
EP  - 678-687
PB  - Blackwell Science
SN  - 09607412
KW  - Arabidopsis thaliana
KW  - chromatin
KW  - epigenetics
KW  - histone modifications
KW  - Nicotiana
KW  - telomeres
UR  - https://pubmed.ncbi.nlm.nih.gov/31834959/
L2  - https://pubmed.ncbi.nlm.nih.gov/31834959/
N2  - Telomeres, nucleoprotein structures at the ends of linear eukaryotic chromosomes, are crucial for the maintenance of genome integrity. In most plants, telomeres consist of conserved tandem repeat units comprising the TTTAGGG motif. Recently, non-canonical telomeres were described in several plants and plant taxons, including the carnivorous plant Genlisea hispidula (TTCAGG/TTTCAGG), the genus Cestrum (Solanaceae; TTTTTTAGGG), and plants from the Asparagales order with either a vertebrate-type telomere repeat TTAGGG or Allium genus-specific CTCGGTTATGGG repeat. We analyzed epigenetic modifications of telomeric histones in plants with canonical and non-canonical telomeres, and further in telomeric chromatin captured from leaves of Nicotiana benthamiana transiently transformed by telomere CRISPR-dCas9-eGFP, and of Arabidopsis thaliana stably transformed with TALE_telo C-3×GFP. Two combinatorial patterns of telomeric histone modifications were identified: (i) an Arabidopsis-like pattern (A. thaliana, G. hispidula, Genlisea nigrocaulis, Allium cepa, Narcissus pseudonarcissus, Petunia hybrida, Solanum tuberosum, Solanum lycopersicum) with telomeric histones decorated predominantly by H3K9me2; (ii) a tobacco-like pattern (Nicotiana tabacum, N. benthamiana, C. elegans) with a strong H3K27me3 signal. Our data suggest that epigenetic modifications of plant telomere-associated histones are related neither to the sequence of the telomere motif nor to the lengths of the telomeres. Nor the phylogenetic position of the species plays the role; representatives of the Solanaceae family are included in both groups. As both patterns of histone marks are compatible with fully functional telomeres in respective plants, we conclude that the described specific differences in histone marks are not critical for telomere functions.
ER  -

ADAMUSOVÁ, Kateřina, Solmaz KOSHRAVI, Satoru FUJIMOTO, Andreas HOUBEN, Sachihiro MATSUNAGA, Jiří FAJKUS and Miloslava FOJTOVÁ. Two combinatorial patterns of telomerase histone marks in plants with canonical and non-canonical telomere repeats. \textit{The Plant Journal}. Blackwell Science, 2020, vol.~102, No~4, p.~678-687. ISSN~0960-7412. Available from: https://dx.doi.org/10.1111/tpj.14653.

Detailed Information on Publication Record