Phospholipid profiling enables to discriminate tumor- and
non-tumor-derived human colon epithelial cells: Phospholipidome
similarities and differences in colon cancer cell lines and in
patient-derived cell samples

J 2020

Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples

HOFMANOVA, Jirina, Josef SLAVIK, Petra OVESNÁ, Zuzana TYLICHOVA, Ladislav DUŠEK et. al.

Basic information

Original name

Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples

Authors

HOFMANOVA, Jirina (203 Czech Republic), Josef SLAVIK (203 Czech Republic), Petra OVESNÁ (203 Czech Republic, belonging to the institution), Zuzana TYLICHOVA (203 Czech Republic), Ladislav DUŠEK (203 Czech Republic, belonging to the institution), Nicol STRAKOVA (203 Czech Republic), Alena VACULOVA HYRSLOVA (203 Czech Republic), Miroslav CIGANEK (203 Czech Republic), Zdeněk KALA (203 Czech Republic), Miroslav JÍRA (203 Czech Republic), Igor PENKA (203 Czech Republic), Jitka KYCLOVÁ (203 Czech Republic), Zdenek KOLAR (203 Czech Republic), Alois KOZUBÍK (203 Czech Republic, belonging to the institution), Miroslav MACHALA (203 Czech Republic) and Jan VONDRACEK (203 Czech Republic, guarantor)

Edition

Plos one, San Francisco, Public Library of Science, 2020, 1932-6203

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.240

RIV identification code

RIV/00216224:14110/20:00116239

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pone.0228010

UT WoS

000534612400041

Keywords in English

HUMAN COLORECTAL-CANCER; FATTY-ACID; ADENOMA; PHOSPHATIDYLCHOLINE; PROTEIN; DIFFERENTIATION; ADENOCARCINOMA; PROLIFERATION; ABNORMALITIES; INVOLVEMENT

Abstract

V originále

Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.

Citovat

HOFMANOVA, Jirina, Josef SLAVIK, Petra OVESNÁ, Zuzana TYLICHOVA, Ladislav DUŠEK, Nicol STRAKOVA, Alena VACULOVA HYRSLOVA, Miroslav CIGANEK, Zdeněk KALA, Miroslav JÍRA, Igor PENKA, Jitka KYCLOVÁ, Zdenek KOLAR, Alois KOZUBÍK, Miroslav MACHALA and Jan VONDRACEK. Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples. Plos one. San Francisco: Public Library of Science, 2020, vol. 15, No 1, p. 1-21. ISSN 1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0228010.

@article{1674766,
   author = {Hofmanova, Jirina and Slavik, Josef and Ovesná, Petra and Tylichova, Zuzana and Dušek, Ladislav and Strakova, Nicol and Vaculova Hyrslova, Alena and Ciganek, Miroslav and Kala, Zdeněk and Jíra, Miroslav and Penka, Igor and Kyclová, Jitka and Kolar, Zdenek and Kozubík, Alois and Machala, Miroslav and Vondracek, Jan},
   article_location = {San Francisco},
   article_number = {1},
   doi = {http://dx.doi.org/10.1371/journal.pone.0228010},
   keywords = {HUMAN COLORECTAL-CANCER; FATTY-ACID; ADENOMA; PHOSPHATIDYLCHOLINE; PROTEIN; DIFFERENTIATION; ADENOCARCINOMA; PROLIFERATION; ABNORMALITIES; INVOLVEMENT},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples},
   url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010},
   volume = {15},
   year = {2020}
}

TY  - JOUR
ID  - 1674766
AU  - Hofmanova, Jirina - Slavik, Josef - Ovesná, Petra - Tylichova, Zuzana - Dušek, Ladislav - Strakova, Nicol - Vaculova Hyrslova, Alena - Ciganek, Miroslav - Kala, Zdeněk - Jíra, Miroslav - Penka, Igor - Kyclová, Jitka - Kolar, Zdenek - Kozubík, Alois - Machala, Miroslav - Vondracek, Jan
PY  - 2020
TI  - Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples
JF  - Plos one
VL  - 15
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - Public Library of Science
SN  - 19326203
KW  - HUMAN COLORECTAL-CANCER
KW  - FATTY-ACID
KW  - ADENOMA
KW  - PHOSPHATIDYLCHOLINE
KW  - PROTEIN
KW  - DIFFERENTIATION
KW  - ADENOCARCINOMA
KW  - PROLIFERATION
KW  - ABNORMALITIES
KW  - INVOLVEMENT
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010
L2  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010
N2  - Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.
ER  -

HOFMANOVA, Jirina, Josef SLAVIK, Petra OVESNÁ, Zuzana TYLICHOVA, Ladislav DUŠEK, Nicol STRAKOVA, Alena VACULOVA HYRSLOVA, Miroslav CIGANEK, Zdeněk KALA, Miroslav JÍRA, Igor PENKA, Jitka KYCLOVÁ, Zdenek KOLAR, Alois KOZUBÍK, Miroslav MACHALA and Jan VONDRACEK. Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples. \textit{Plos one}. San Francisco: Public Library of Science, 2020, vol.~15, No~1, p.~1-21. ISSN~1932-6203. Available from: https://dx.doi.org/10.1371/journal.pone.0228010.

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