Phospholipid profiling enables to discriminate tumor- and
non-tumor-derived human colon epithelial cells: Phospholipidome
similarities and differences in colon cancer cell lines and in
patient-derived cell samples

J 2020

Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples

HOFMANOVA, Jirina; Josef SLAVIK; Petra OVESNÁ; Zuzana TYLICHOVA; Ladislav DUŠEK et. al.

Basic information

Original name

Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples

Authors

Edition

Plos one, San Francisco, Public Library of Science, 2020, 1932-6203

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30204 Oncology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 3.240

RIV identification code

RIV/00216224:14110/20:00116239

Organization unit

Faculty of Medicine

DOI

https://doi.org/10.1371/journal.pone.0228010

UT WoS

000534612400041

EID Scopus

2-s2.0-85078711169

Keywords in English

HUMAN COLORECTAL-CANCER; FATTY-ACID; ADENOMA; PHOSPHATIDYLCHOLINE; PROTEIN; DIFFERENTIATION; ADENOCARCINOMA; PROLIFERATION; ABNORMALITIES; INVOLVEMENT

Abstract

In the original language

Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.

Cite

HOFMANOVA, Jirina; Josef SLAVIK; Petra OVESNÁ; Zuzana TYLICHOVA; Ladislav DUŠEK; Nicol STRAKOVA; Alena VACULOVA HYRSLOVA; Miroslav CIGANEK; Zdeněk KALA; Miroslav JÍRA; Igor PENKA; Jitka KYCLOVÁ; Zdenek KOLAR; Alois KOZUBÍK; Miroslav MACHALA and Jan VONDRACEK. Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples. Plos one. San Francisco: Public Library of Science, 2020, vol. 15, No 1, p. 1-21. ISSN 1932-6203. Available from: https://doi.org/10.1371/journal.pone.0228010.

@article{1674766,
   author = {Hofmanova, Jirina and Slavik, Josef and Ovesná, Petra and Tylichova, Zuzana and Dušek, Ladislav and Strakova, Nicol and Vaculova Hyrslova, Alena and Ciganek, Miroslav and Kala, Zdeněk and Jíra, Miroslav and Penka, Igor and Kyclová, Jitka and Kolar, Zdenek and Kozubík, Alois and Machala, Miroslav and Vondracek, Jan},
   article_location = {San Francisco},
   article_number = {1},
   doi = {https://doi.org/10.1371/journal.pone.0228010},
   keywords = {HUMAN COLORECTAL-CANCER; FATTY-ACID; ADENOMA; PHOSPHATIDYLCHOLINE; PROTEIN; DIFFERENTIATION; ADENOCARCINOMA; PROLIFERATION; ABNORMALITIES; INVOLVEMENT},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples},
   url = {https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010},
   volume = {15},
   year = {2020}
}

TY  - JOUR
ID  - 1674766
AU  - Hofmanova, Jirina - Slavik, Josef - Ovesná, Petra - Tylichova, Zuzana - Dušek, Ladislav - Strakova, Nicol - Vaculova Hyrslova, Alena - Ciganek, Miroslav - Kala, Zdeněk - Jíra, Miroslav - Penka, Igor - Kyclová, Jitka - Kolar, Zdenek - Kozubík, Alois - Machala, Miroslav - Vondracek, Jan
PY  - 2020
TI  - Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples
JF  - Plos one
VL  - 15
IS  - 1
SP  - 1-21
EP  - 1-21
PB  - Public Library of Science
SN  - 19326203
KW  - HUMAN COLORECTAL-CANCER
KW  - FATTY-ACID
KW  - ADENOMA
KW  - PHOSPHATIDYLCHOLINE
KW  - PROTEIN
KW  - DIFFERENTIATION
KW  - ADENOCARCINOMA
KW  - PROLIFERATION
KW  - ABNORMALITIES
KW  - INVOLVEMENT
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010
L2  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0228010
N2  - Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.
ER  -

HOFMANOVA, Jirina; Josef SLAVIK; Petra OVESNÁ; Zuzana TYLICHOVA; Ladislav DUŠEK; Nicol STRAKOVA; Alena VACULOVA HYRSLOVA; Miroslav CIGANEK; Zdeněk KALA; Miroslav JÍRA; Igor PENKA; Jitka KYCLOVÁ; Zdenek KOLAR; Alois KOZUBÍK; Miroslav MACHALA and Jan VONDRACEK. Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples. \textit{Plos one}. San Francisco: Public Library of Science, 2020, vol.~15, No~1, p.~1-21. ISSN~1932-6203. Available from: https://doi.org/10.1371/journal.pone.0228010.

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