HOFMANOVA, Jirina, Josef SLAVIK, Petra OVESNÁ, Zuzana TYLICHOVA, Ladislav DUŠEK, Nicol STRAKOVA, Alena VACULOVA HYRSLOVA, Miroslav CIGANEK, Zdeněk KALA, Miroslav JÍRA, Igor PENKA, Jitka KYCLOVÁ, Zdenek KOLAR, Alois KOZUBÍK, Miroslav MACHALA and Jan VONDRACEK. Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples. Plos one. San Francisco: Public Library of Science, vol. 15, No 1, p. 1-21. ISSN 1932-6203. doi:10.1371/journal.pone.0228010. 2020.
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Basic information
Original name Phospholipid profiling enables to discriminate tumor- and non-tumor-derived human colon epithelial cells: Phospholipidome similarities and differences in colon cancer cell lines and in patient-derived cell samples
Authors HOFMANOVA, Jirina (203 Czech Republic), Josef SLAVIK (203 Czech Republic), Petra OVESNÁ (203 Czech Republic, belonging to the institution), Zuzana TYLICHOVA (203 Czech Republic), Ladislav DUŠEK (203 Czech Republic, belonging to the institution), Nicol STRAKOVA (203 Czech Republic), Alena VACULOVA HYRSLOVA (203 Czech Republic), Miroslav CIGANEK (203 Czech Republic), Zdeněk KALA (203 Czech Republic), Miroslav JÍRA (203 Czech Republic), Igor PENKA (203 Czech Republic), Jitka KYCLOVÁ (203 Czech Republic), Zdenek KOLAR (203 Czech Republic), Alois KOZUBÍK (203 Czech Republic, belonging to the institution), Miroslav MACHALA (203 Czech Republic) and Jan VONDRACEK (203 Czech Republic, guarantor).
Edition Plos one, San Francisco, Public Library of Science, 2020, 1932-6203.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 30204 Oncology
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 3.240
RIV identification code RIV/00216224:14110/20:00116239
Organization unit Faculty of Medicine
Doi http://dx.doi.org/10.1371/journal.pone.0228010
UT WoS 000534612400041
Keywords in English HUMAN COLORECTAL-CANCER; FATTY-ACID; ADENOMA; PHOSPHATIDYLCHOLINE; PROTEIN; DIFFERENTIATION; ADENOCARCINOMA; PROLIFERATION; ABNORMALITIES; INVOLVEMENT
Tags 14119612, podil, rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 13/5/2021 07:59.
Abstract
Identification of changes of phospholipid (PL) composition occurring during colorectal cancer (CRC) development may help us to better understand their roles in CRC cells. Here, we used LC-MS/MS-based PL profiling of cell lines derived from normal colon mucosa, or isolated at distinct stages of CRC development, in order to study alterations of PL species potentially linked with cell transformation. We found that a detailed evaluation of phosphatidylinositol (PI) and phosphatidylserine (PS) classes allowed us to cluster the studied epithelial cell lines according to their origin: i) cells originally derived from normal colon tissue (NCM460, FHC); ii) cell lines derived from colon adenoma or less advanced differentiating adenocarcinoma cells (AA/C1, HT-29); or, iii) cells obtained by in vitro transformation of adenoma cells and advanced colon adenocarcinoma cells (HCT-116, AA/C1/SB10, SW480, SW620). Although we tentatively identified several PS and PI species contributing to cell line clustering, full PI and PS profiles appeared to be a key to the successful cell line discrimination. In parallel, we compared PL composition of primary epithelial (EpCAM-positive) cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients, with PL profiles of cell lines derived from normal colon mucosa (NCM460) and from colon adenocarcinoma (HCT-116, SW480) cells, respectively. In general, higher total levels of all PL classes were observed in tumor cells. The overall PL profiles of the cell lines, when compared with the respective patient-derived cells, exhibited similarities. Nevertheless, there were also some notable differences in levels of individual PL species. This indicated that epithelial cell lines, derived either from normal colon tissue or from CRC cells, could be employed as models for functional lipidomic analyses of colon cells, albeit with some caution. The biological significance of the observed PL deregulation, or their potential links with specific CRC stages, deserve further investigation.
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