Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Katarzyna STEFANSKA, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Dorota BUKOWSKA, Maciej ZABEL, Paul MOZDZIAK, Michal NOWICKI, Bartosz KEMPISTY and Pawel ANTOSIK. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. Biomed Research International. London: Hindawi Publishing Corporation, 2020, vol. 2020, FEB 2020, p. 1-15. ISSN 2314-6133. Available from: https://dx.doi.org/10.1155/2020/7120375.

Basic information

• Original name: Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model
• Authors: WOJTANOWICZ-MARKIEWICZ, Katarzyna (616 Poland), Magdalena KULUS (616 Poland), Sandra KNAP (616 Poland), Ievgenia KOCHEROVA (616 Poland), Maurycy JANKOWSKI (616 Poland), Katarzyna STEFANSKA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Hanna PIOTROWSKA-KEMPISTY (616 Poland), Dorota BUKOWSKA (616 Poland), Maciej ZABEL (616 Poland), Paul MOZDZIAK (840 United States of America), Michal NOWICKI (616 Poland), Bartosz KEMPISTY (616 Poland, guarantor) and Pawel ANTOSIK (616 Poland).
• Edition: Biomed Research International, London, Hindawi Publishing Corporation, 2020, 2314-6133.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10606 Microbiology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.411
• RIV identification code: RIV/00216224:14110/20:00116242
• Organization unit: Faculty of Medicine
• Doi: http://dx.doi.org/10.1155/2020/7120375
• UT WoS: 000515519100003
• Keywords in English: MESSENGER-RNA; ESTROUS-CYCLE; FEMALE; JUNCTIONS; UTERUS
• Tags: 14110411, GPK, GPKgyn, rivok
• Tags: International impact, Reviewed

Abstract

Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx's behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.