Expression of Selected Connexin and Aquaporin Genes and
Real-Time Proliferation of Porcine Endometrial Luminal
Epithelial Cells in Primary Culture Model

J 2020

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI et. al.

Basic information

Original name

Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model

Authors

WOJTANOWICZ-MARKIEWICZ, Katarzyna (616 Poland), Magdalena KULUS (616 Poland), Sandra KNAP (616 Poland), Ievgenia KOCHEROVA (616 Poland), Maurycy JANKOWSKI (616 Poland), Katarzyna STEFANSKA (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), Hanna PIOTROWSKA-KEMPISTY (616 Poland), Dorota BUKOWSKA (616 Poland), Maciej ZABEL (616 Poland), Paul MOZDZIAK (840 United States of America), Michal NOWICKI (616 Poland), Bartosz KEMPISTY (616 Poland, guarantor) and Pawel ANTOSIK (616 Poland)

Edition

Biomed Research International, London, Hindawi Publishing Corporation, 2020, 2314-6133

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.411

RIV identification code

RIV/00216224:14110/20:00116242

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1155/2020/7120375

UT WoS

000515519100003

Keywords in English

MESSENGER-RNA; ESTROUS-CYCLE; FEMALE; JUNCTIONS; UTERUS

Abstract

V originále

Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx's behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.

Citovat

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Katarzyna STEFANSKA, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Dorota BUKOWSKA, Maciej ZABEL, Paul MOZDZIAK, Michal NOWICKI, Bartosz KEMPISTY and Pawel ANTOSIK. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. Biomed Research International. London: Hindawi Publishing Corporation, 2020, vol. 2020, FEB 2020, p. 1-15. ISSN 2314-6133. Available from: https://dx.doi.org/10.1155/2020/7120375.

@article{1674771,
   author = {WojtanowiczandMarkiewicz, Katarzyna and Kulus, Magdalena and Knap, Sandra and Kocherova, Ievgenia and Jankowski, Maurycy and Stefanska, Katarzyna and Ješeta, Michal and PiotrowskaandKempisty, Hanna and Bukowska, Dorota and Zabel, Maciej and Mozdziak, Paul and Nowicki, Michal and Kempisty, Bartosz and Antosik, Pawel},
   article_location = {London},
   article_number = {FEB 2020},
   doi = {http://dx.doi.org/10.1155/2020/7120375},
   keywords = {MESSENGER-RNA; ESTROUS-CYCLE; FEMALE; JUNCTIONS; UTERUS},
   language = {eng},
   issn = {2314-6133},
   journal = {Biomed Research International},
   title = {Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model},
   url = {http://downloads.hindawi.com/journals/bmri/2020/7120375.pdf},
   volume = {2020},
   year = {2020}
}

TY  - JOUR
ID  - 1674771
AU  - Wojtanowicz-Markiewicz, Katarzyna - Kulus, Magdalena - Knap, Sandra - Kocherova, Ievgenia - Jankowski, Maurycy - Stefanska, Katarzyna - Ješeta, Michal - Piotrowska-Kempisty, Hanna - Bukowska, Dorota - Zabel, Maciej - Mozdziak, Paul - Nowicki, Michal - Kempisty, Bartosz - Antosik, Pawel
PY  - 2020
TI  - Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model
JF  - Biomed Research International
VL  - 2020
IS  - FEB 2020
SP  - 1-15
EP  - 1-15
PB  - Hindawi Publishing Corporation
SN  - 23146133
KW  - MESSENGER-RNA
KW  - ESTROUS-CYCLE
KW  - FEMALE
KW  - JUNCTIONS
KW  - UTERUS
UR  - http://downloads.hindawi.com/journals/bmri/2020/7120375.pdf
L2  - http://downloads.hindawi.com/journals/bmri/2020/7120375.pdf
N2  - Luminal epithelial cells are the first embryonic-maternal contact site undergoing very specific changes associated with reproductive processes. Cells prepare for embryo development by increasing their volume, with the help of aquaporins that provide a transcellular path of rapid water movement during the secretion and absorption of fluids, as well as connexins enabling the flow of inorganic ions and small molecules. In this work, we have examined how AQPs and Cx's behave in luminal epithelium primary cell culture. Cells obtained from porcine specimen during slaughter were primarily in vitro cultured for 7 days. Their proliferation patterns were then analyzed using RTCA, with the expression of genes of interest evaluated with the use of immunofluorescence and RT-qPCR. The results of these changes of gene of interest expression were analyzed on each of the seven days of the porcine luminal primary cell culture. Our study showed that the significant changes were noted in the case of Cx43, whose level of protein expression and distribution increases after 120 hours of culture, when the cells enter the lag phase, and maintains an upward trend until the end of the culture. We noted an increase in AQP4, AQP7, AQP8, and AQP11 levels throughout the entire culture period, while the largest differences in expression were found in AQP3, AQP4, and AQP10. The obtained results could become a point of reference for further in vivo and clinical research. Experiments conducted with these proteins showed that they influence the endometrial fluid content during the oestrous cycle and participate in the process of angiogenesis, which intensifies during endometrial development.
ER  -

WOJTANOWICZ-MARKIEWICZ, Katarzyna, Magdalena KULUS, Sandra KNAP, Ievgenia KOCHEROVA, Maurycy JANKOWSKI, Katarzyna STEFANSKA, Michal JEŠETA, Hanna PIOTROWSKA-KEMPISTY, Dorota BUKOWSKA, Maciej ZABEL, Paul MOZDZIAK, Michal NOWICKI, Bartosz KEMPISTY and Pawel ANTOSIK. Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model. \textit{Biomed Research International}. London: Hindawi Publishing Corporation, 2020, vol.~2020, FEB 2020, p.~1-15. ISSN~2314-6133. Available from: https://dx.doi.org/10.1155/2020/7120375.

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