Detailed Information on Publication Record
2020
New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation
BRAZERT, M., W. KRANC, M. J. NAWROCKI, P. SUJKA-KORDOWSKA, A. KONWERSKA et. al.Basic information
Original name
New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation
Authors
BRAZERT, M. (616 Poland), W. KRANC (616 Poland), M. J. NAWROCKI (616 Poland), P. SUJKA-KORDOWSKA (616 Poland), A. KONWERSKA (616 Poland), M. JANKOWSKI (616 Poland), I. KOCHEROVA (616 Poland), P. CELICHOWSKI (616 Poland), Michal JEŠETA (203 Czech Republic, belonging to the institution), K. OZEGOWSKA (616 Poland), P. ANTOSIK (616 Poland), D. BUKOWSKA (616 Poland), M. T. SKOWRONSKI (616 Poland), L. PAWELCZYK (616 Poland), M. ZABEL (616 Poland), H. PIOTROWSKA-KEMPISTY (616 Poland), M. NOWICKI (616 Poland), B. KEMPISTY (616 Poland, guarantor) and M. BRUSKA (616 Poland)
Edition
Molecular Medicine Reports, Athens, SPANDIDOS PUBL LTD, 2020, 1791-2997
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10601 Cell biology
Country of publisher
Greece
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 2.952
RIV identification code
RIV/00216224:14110/20:00116243
Organization unit
Faculty of Medicine
UT WoS
000515676300061
Keywords in English
pig; oocyte; RNA; transcription; in vitro maturation
Tags
International impact, Reviewed
Změněno: 19/8/2020 13:24, Mgr. Tereza Miškechová
Abstract
V originále
Oocyte maturation is essential for proper fertilization, embryo implantation and early development. While the physiological conditions of these processes are relatively well-known, its exact molecular mechanisms remain widely undiscovered. Oocyte growth, differentiation and maturation are therefore the subject of scientific debate. Precious literature has indicated that the oocyte itself serves a regulatory role in the mechanisms underlying these processes. Hence, the present study performed expression microarrays to analyze the complete transcriptome of porcine oocytes during their in vitro maturation (IVM). Pig material was used for experimentation, as it possesses similarities to the reproductive processes and general genetic proximities of Sus scrofa to human. Oocytes, isolated from the ovaries of slaughtered animals were assessed via the Brilliant Cresyl Blue test and directed to IVM. A number of oocytes were left to be analyzed as the 'before IVM' group. Oocyte mRNA was isolated and used for microarray analysis, which was subsequently validated via RT-qPCR. The current study particularly focused on genes belonging to 'positive regulation of transcription, DNA-dependent', 'positive regulation of gene expression', 'positive regulation of macromolecule metabolic process' and 'positive regulation of transcription from RNA polymerase II promoter' ontologies. FOS, VEGFA, ESR1, AR, CCND2, EGR2, ENDRA, GJA1, INHBA, IHH, INSR, APP, WWTR1, SMARCA1, NFAT5, SMAD4, MAP3K1, EGR1, RORA, ECE1, NR5A1, KIT, IKZF2, MEF2C, SH3D19, MITF and PSMB4 were all determined to be significantly altered (fold change, >|2|; P<0.05) among these groups, with their downregulation being observed after IVM. Genes with the most altered expressions were analyzed and considered to be potential markers of maturation associated with transcription regulation and macromolecule metabolism process.