J 2021

Redox and Epigenetics in Human Pluripotent Stem Cells Differentiation

GIALLONGO, Sebastiano, Daniela ŘEHÁKOVÁ, Marco RAFFAELE, Oriana LO RE, Irena KOUTNÁ et. al.

Basic information

Original name

Redox and Epigenetics in Human Pluripotent Stem Cells Differentiation

Authors

GIALLONGO, Sebastiano (380 Italy, belonging to the institution), Daniela ŘEHÁKOVÁ (203 Czech Republic, belonging to the institution), Marco RAFFAELE (380 Italy), Oriana LO RE (380 Italy), Irena KOUTNÁ (203 Czech Republic, belonging to the institution) and Manlio VINCIGUERRA (380 Italy, guarantor, belonging to the institution)

Edition

ANTIOXIDANTS & REDOX SIGNALING, NEW ROCHELLE, MARY ANN LIEBERT, INC, 2021, 1523-0864

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 7.468

RIV identification code

RIV/00216224:14110/21:00120673

Organization unit

Faculty of Medicine

UT WoS

000555879000001

Keywords in English

induced pluripotent stem cells (iPSCs); oxidative stress; DNA damage

Tags

International impact, Reviewed
Změněno: 24/10/2022 10:17, Mgr. Tereza Miškechová

Abstract

V originále

Significance:Since their discovery, induced pluripotent stem cells (iPSCs) had generated considerable interest in the scientific community for their great potential in regenerative medicine, disease modeling, and cell-based therapeutic approach, due to their unique characteristics of self-renewal and pluripotency. Recent Advances:Technological advances in iPSC genome-wide epigenetic profiling led to the elucidation of the epigenetic control of cellular identity during nuclear reprogramming. Moreover, iPSC physiology and metabolism are tightly regulated by oxidation-reduction events that mainly occur during the respiratory chain. In theory, iPSC-derived differentiated cells would be ideal for stem cell transplantation as autologous cells from donors, as the risks of rejection are minimal. Critical Issues:However, iPSCs experience high oxidative stress that, in turn, confers a high risk of increased genomic instability, which is most often linked to DNA repair deficiencies. Genomic instability has to be assessed before iPSCs can be used in therapeutic designs. Future Directions:This review will particularly focus on the links between redox balance and epigenetic modifications-in particular based on the histone variant macroH2A1-that determine DNA damage response in iPSCs and derived differentiated cells, and that might be exploited to decrease the teratogenic potential on iPSC transplantation.

Links

MUNI/A/1325/2020, interní kód MU
Name: Biomedicínské vědy
Investor: Masaryk University
90128, large research infrastructures
Name: CZECRIN III